LOCUS       sequence-200754-        4499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' element for Gateway cloning, 5 single nucleotide mutations 
            compared to the reference sequence, but none of them change the 
            amino acidtruncation aa416-1033(end) 1854 bp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, 
            Norden C, Wilsch-Brauninger M, Huttner WB
  TITLE     Non-canonical features of the Golgi apparatus in bipolar epithelial 
            neural stem cells.
  JOURNAL   Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.
  PUBMED    26879757
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:21206. doi"; pages: " 
            10.1038/srep21206"
COMMENT     Sequence Label: p3E DN Kif5a
FEATURES             Location/Qualifiers
     source          1..4499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..747)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2608..2703)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2740..2759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2741..2759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2764..2780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2882..2901)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2893..3702
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2960..2979)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3849..4437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4338..4357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggctt cctctatcgt ggtgcgcatc tcagaggaag
      781 agcggcagaa atacgaggaa gagatccgca aactctaccg ccagctggac gacaaggatg
      841 atgagatcaa tctccagtgt cagctggtgg agaagctgaa ggagcagatt ctggatcagg
      901 atgagctgct ggcctccacc cgcggcgaca gtgataaggt acagacggag ctgggtcgtc
      961 tgcagacgga gagcgagagc gccaaggcgg aggtgcggga ggtgctgcag gcgctggagg
     1021 agctggccgt caactacgac cagaagagcc aggaggtgga ggagaagagt ctgcacaaca
     1081 aacagctggc cgagcagctc agccacaaga tggccaacct gatggagctg gaggcggagc
     1141 tagcgcagat gcaggaagtg agctcacagc agaggaagcg catcgctgat gtgttgaacg
     1201 gcctgatgaa ggacctcagc gagttcagca gcatcgtcgg aaacggagaa ctcaagctac
     1261 cggtggagat cagcggtgcg attgaggagg agttcaccgt ggcacgtctc tacatcagca
     1321 agattaaatc tgaggtgaag tcgatggtga agcgctgccg gcagctggag aacatgcagc
     1381 tggagtgcca tcgcaagatg gaggagacca gccgggagct gtcgtcatgc caactgctca
     1441 tctcacagca tgaggcaaag atccgttctc tcacagaata catgcagaac gtggagcaga
     1501 agaagagaca gctggaggac aactatgatg ctctcagtga agaactctac acactgcaga
     1561 actctgagag tcatgtggca gagctggacg gaggagagaa ggcggacaga gaggctgatg
     1621 ggaaaaaggc cgctgttcgt ccagctctgc atgcggagtc tcgtcagacg cagctcagcc
     1681 gcctgcggga tgaaattaac gagaagcaga gactcatcga cgagctcaca gataagaacc
     1741 aggctctgga gttggagctg gctcacgtgc gctccagttt cagcaatctg aagctgcagg
     1801 acgacagcaa gagcgcctgt ctggagcaga tgtcgtttca acaggagaga cacgagcagt
     1861 ccaaacagga tctcaagggc cttgaggaga ctgttgctcg ggaactccag accctccaca
     1921 acctgcgcaa gctgttcgtt caagacctca cgactcgggt taaaaaaagt tcagaaattg
     1981 gacctgatga tagtgggggg tctaacaccc agaagcagaa gatttccttt cttgagaaca
     2041 acctggatca gcttacaaag gttcataaac agctggtacg tgataatgca gatctccgct
     2101 gtgagcttcc gaagctggag aaacgtcttc gttctacggc tgagcgggtt cgggcgctgg
     2161 agacagcact gaaggacgcc aaacagggcg cgatgaatga ccggcgccgg taccagcagg
     2221 aggtggagcg catccgggac gccatgaggc tgcgttaccc actgcgccgg gccaacgccg
     2281 ctcagatcgc taagccagtg agacccgggc atcacgtcac cgccacatcg cccaccagct
     2341 tctccgtcac acgcgcaagc aaaccggcca cgtcttacag caatgcccga ttcctgcagg
     2401 acgagaagat ggagaacagc cagaagacca acactgttgg atacagcact gtgagatcca
     2461 tagacatact gagctcctgt ccgctcgacg tggagaacgg aaacagcaca gatattaatg
     2521 ataacaggag tgatgttcac tgcggcagca tggtggacga tgcctctcga gtgtacatca
     2581 tgcagcagga gactgcagcc agttaagcaa ctttattata caaagttggc attataaaaa
     2641 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2701 tttggagctc catggtagcg ttaacgcggc cgcgatatcc cctatagtga gtcgtattac
     2761 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2821 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     2881 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     2941 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     3001 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     3061 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3121 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3181 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3241 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3301 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3361 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3421 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3481 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3541 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3601 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3661 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     3721 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     3781 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3961 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4201 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     4261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
