LOCUS       sequence-200823-        1659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR176; Opto-GPR176; F2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR176
FEATURES             Location/Qualifiers
     source          1..1659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcactt gccgcacaac cgtgttcaaa
      301 tctgtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg ctctatccac tggagaggaa aatatctgat
      541 gccaaggcca tcatgggcgt cgccttcacc tgggtcatgg ctctggcctg tgccgcgccc
      601 cccctcgtcg gctggtccag gtacatcccg gagggcatgc agtgctcgtg cgggattgac
      661 tactacacgc cccacgagga aaccaacaat gagtcgttcg tcatctacat gttcgtggtc
      721 cacttcatca tccccctgat tgtcatattc ttctgctacg gactgatccg acgggccctg
      781 agtgccagcc agaagaagaa ggtcatcata gcagcgctcc ggaccccaca gaacaccatc
      841 tctattccct atgcctccca gcgggaggcc gagctgcacc gcatggtgat catcatggtc
      901 atcgctttcc taatctgctg gctgccctac gctggggtgg cgttctacat cttcacccat
      961 cagggctctg actttggccc catcttcatg accatcccgg ctttctttgc caagacttct
     1021 gccgtctaca accccgtcat ctacatcatg atgaacaagc agttccgcaa gtgcttgata
     1081 gggaccctgg tgcaactaca ccaccgatac agtcgccgta atgtggtcag tacagggagt
     1141 ggcatggctg aggccagcct ggaacccagc atacgctcgg gtagccagct cctggagatg
     1201 ttccacattg ggcagcagca gatctttaag cccacagagg atgaggaaga gagtgaggcc
     1261 aagtacattg gttcagctga cttccaggcc aaggagatat ttagcacctg cctggaggga
     1321 gagcaggggc cacagtttgc gccctctgcc ccacccctga gcacagtgga ctctgtatcc
     1381 caggtggcac cggcagcccc tgtggaacct gaaacattcc ctgataagta ttccctgcag
     1441 tttggctttg ggccttttga gttgcctcct cagtggctct cagaaacccg aaacagcaag
     1501 aagcggctgc ttcccccctt gggcaacacc ccagaggagc tgatccagac aaaggtgccc
     1561 aaggtaggca gggtggagcg gaagatgagc agaaacaata aagtgagcat ttttccaaag
     1621 gtggattcca cagaaaccag ccaagtggcg cctgcctaa
//
