LOCUS       sequence-200825-        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR183; Opto-GPR183; F4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR183
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcattg ttcaaaacag gaaaaaaatc
      301 aactctctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg gtgcaccctc tacgctacaa caagataaaa
      541 aggattgaac atgccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaca gatctgctgc
      781 aaactcttca gaactgccaa acaaaaccca ctcactgaga aatctggtgt aaacaaaaag
      841 gctctccgca tggtgatcat catggtcatc gctttcctaa tctgctggct gccctacgct
      901 ggggtggcgt tctacatctt cacccatcag ggctctgact ttggccccat cttcatgacc
      961 atcccggctt tctttgccaa gacttctgcc gtctacaacc ccgtcatcta catcatgatg
     1021 aacaagcagt tccgcagaaa ggttatgagg atgctgaaac ggcaagtcag tgtatcgatt
     1081 tctagtgctg tgaagtcagc ccctgaagaa aattcacgtg aaatgacaga aacgcagatg
     1141 atgatacatt ccaagtcctc aaatggaaag acagaaacca gccaagtggc gcctgcctaa
//
