LOCUS       sequence-200830-        1482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-D1R; Opto-D1R; F9; 720).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-D1R
FEATURES             Location/Qualifiers
     source          1..1482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtca tcaggtttcg acacctgcgg
      301 tccaaggtgc tcaactacat cctgctcaac ctggccgtgg ccgacctctt catggtgttc
      361 gggggcttca ccaccaccct ctacacctct ctgcacgggt acttcgtctt tgggcccacg
      421 ggctgcaacc tggagggctt ctttgccacc ttgggcggtg aaattgcact gtggtccttg
      481 gtggtcctgg ccatcgagcg gtacgtggtg gtgtccagcc ctttccagta tgagaggaag
      541 atgactccga aggcagccat catgggcgtc gccttcacct gggtcatggc tctggcctgt
      601 gccgcgcccc ccctcgtcgg ctggtccagg tacatcccgg agggcatgca gtgctcgtgc
      661 gggattgact actacacgcc ccacgaggaa accaacaatg agtcgttcgt catctacatg
      721 ttcgtggtcc acttcatcat ccccctgatt gtcatattct tctgctacgg aagtatctac
      781 aggattgccc agaagcaaat ccggcgcatc tcagctttgg agagggcagc agtccatgcc
      841 aagaactgcc agaccaccac aggtaatgga aaccctgtcg aatgctctca atcagaaagt
      901 tcctttaaga tgtcctttaa gagggagact aaagtcctgc gcatggtgat catcatggtc
      961 atcgctttcc taatctgctg gctgccctac gctggggtgg cgttctacat cttcacccat
     1021 cagggctctg actttggccc catcttcatg accatcccgg ctttctttgc caagacttct
     1081 gccgtctaca accccgtcat ctacatcatg atgaacaagc agttccgcaa ggcattctcg
     1141 accctcttag gatgctatag actctgccct acaacgaata atgccataga gactgtaagc
     1201 atcaacaaca acggggctgt gatgttttcc agccaccatg agccccgagg ctccatctcc
     1261 aaggactgta atctggttta cctgatccct catgctgtgg gctcctctga ggacctgaag
     1321 agggaggagg ccggtggcat acctaagcca ctggagaagc tgtccccggc cttatcggtc
     1381 atattggact atgacaccga tgtctctcta gaaaagatcc aacccgttac ccacagcgga
     1441 cagcattcaa ccacagaaac cagccaagtg gcgcctgcct aa
//
