LOCUS       sequence-200832-        1554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-M1R; Opto-M1R; F11; 722).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-M1R
FEATURES             Location/Qualifiers
     source          1..1554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcttca aggtcaacac ggagctcaag
      301 acagtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg actcggcccc tgagctaccg tgccaagcgc
      541 acaccccgcc gggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggacg catctaccgg
      781 gagacagaga accgagcacg ggagctggca gcccttcagg gctccgaaac gccaggcaaa
      841 gggggtggca gcagcagcag ctcagagagg tcccagccag gggctgaggg ctcaccagag
      901 actcctccag gccgctgctg tcgctgctgc cgggccccca ggctgctgca ggcctacagc
      961 tggaaggaag aagaggaaga ggacgaaggc tccatggagt ccctcacatc ctcagaggga
     1021 gaggagcctg gctccgaagt ggtgatcaag atgccaatgg tggaccccga ggcacaggcc
     1081 cccaccaagc agcccccacg gagctcccca aatacagtca agaggccgac taagaaaggg
     1141 cgtgatcgag ctggcaaggg ccagaagccc cgtggaaagg agcagctggc caagcggaaa
     1201 accttctcgc tggtcaagga gaagaaggcg gctcgcatgg tgatcatcat ggtcatcgct
     1261 ttcctaatct gctggctgcc ctacgctggg gtggcgttct acatcttcac ccatcagggc
     1321 tctgactttg gccccatctt catgaccatc ccggctttct ttgccaagac ttctgccgtc
     1381 tacaaccccg tcatctacat catgatgaac aagcagttcc gcgacacctt tcgcctgctg
     1441 ctgctttgcc gctgggacaa gaggcgctgg cgcaagatcc ccaagcgccc tggctccgtg
     1501 caccgcactc cctcccgcca atgcacagaa accagccaag tggcgcctgc ctaa
//
