LOCUS       sequence-200989-        8546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing synonymous tandem PP7 coat protein 
            fused to synonymous tandem GFP. Used to label reporter mRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger 
            J, Stadler MB, Giorgetti L, Chao JA
  TITLE     The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in
            Single Cells.
  JOURNAL   Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 
            10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
  PUBMED    29056324
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.09.030"; journalName: "Mol Cell"; date: 
            "2017-11-2- 2"; volume: "68"; issue: "3"; pages: "615-625.e9"
COMMENT     Sequence Label: phage UbiC NLS HA stdPCP stdGFP
FEATURES             Location/Qualifiers
     source          1..8546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    675..800
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1297..1530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1715..1759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2052..2169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2212..2610
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2628..2648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3447..3456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3453..3455,3456..3458,3459..4169)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3453..3455,3456..3458,3459..4169)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3498..3519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3759..3778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4106..4127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4203..4916
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4203..4913
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    4930..5518
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4983..5003)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5401..5412)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5593..5826
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5945..5963)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6031..6135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6136..6204,6205..6996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6354..6373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7167..7755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7656..7675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7909..7926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8166..8187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8202..8207,8208..8225,8226..8232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8240..8256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8245..8267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8264..8280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8264..8280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8330..8349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(8541..8546,1..628)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
       61 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
      121 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
      181 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
      241 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
      301 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
      361 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
      421 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
      481 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      541 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      601 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      661 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      721 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      781 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      841 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      901 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
      961 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1021 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1081 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1141 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1201 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1261 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1321 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1381 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1441 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1501 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1561 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1621 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1681 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1741 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1801 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1861 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1921 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1981 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     2041 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2101 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2161 caaaattttc gggtttatta cagggacagc agagatccag tttggactag tggcctccgc
     2221 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     2281 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     2341 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     2401 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     2461 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     2521 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     2581 tgtttgtgga tcgctgtgat cgtcacttgg cggccgccac catgggccca aaaaagaaaa
     2641 gaaaagttgg ctaccccgat tacaaggatg acgacgataa ggcctgcagt ttggcttcca
     2701 aaactatcgt tctgagcgta ggtgaagcga ccagaacgtt aactgaaatt caatctacag
     2761 cagacaggca aatatttgaa gagaaagtcg gtccattagt agggaggtta agattgacag
     2821 ctagcttaag acaaaatgga gctaaaactg catacagggt caatttaaaa ttggatcaag
     2881 cagatgtcgt tgatagcggt ctacctaagg ttagatacac gcaagtgtgg tcacacgatg
     2941 tcactattgt cgcgaattct accgaagcta gtagaaaatc attgtatgat ttaacaaaat
     3001 ccttggtggc tacctctcag gtggaagatc ttgttgtgaa tttagttcca ttaggtaggg
     3061 cggatccgct agcctccaaa accatcgttc tttcggtcgg cgaggctact cgcactctga
     3121 ctgagatcca gtccaccgca gaccgtcaga tcttcgaaga gaaggtcggg cctctggtgg
     3181 gtcggctgcg cctcacggct tcgctccgtc aaaacggagc caagaccgcg tatcgcgtca
     3241 acctaaaact ggatcaggcg gacgtcgttg attccggact tccgaaagtg cgctacactc
     3301 aggtatggtc gcacgacgtg acaatcgttg cgaatagcac cgaggcctcg cgcaaatcgt
     3361 tgtacgattt gaccaagtcc ctcgtcgcga cctcgcaggt cgaagatctt gtcgtcaacc
     3421 ttgtgccgct gggccgtcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     3481 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3541 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3601 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3661 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3721 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3781 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3841 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3901 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3961 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4021 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4081 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4141 tcactctcgg catggacgag ctgtacaagg gcacaggtgc atgcggttca ggttcatcag
     4201 gctctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg
     4261 atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta
     4321 aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag
     4381 tcactacttt aacttatggt gttcaatgtt tttctagata cccagatcat atgaaacaac
     4441 atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca
     4501 aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta
     4561 atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat
     4621 tggaatacaa ctataactct cacaatgttt acatcatggc tgacaaacaa aagaatggta
     4681 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa ttagctgacc
     4741 attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact
     4801 tatccactca atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt
     4861 tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac aaataaatcg
     4921 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4981 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5041 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5101 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5161 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5221 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5281 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5341 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5401 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5461 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5521 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5581 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5641 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5701 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5761 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5821 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5881 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     5941 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6001 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6361 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     6421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7861 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7921 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7981 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8041 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8101 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8161 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8221 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8281 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8341 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     8401 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     8461 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     8521 tgtactacaa acttagtagt tggaag
//
