LOCUS       sequence-201135-        9357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human beta 2 integrin tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Nordenfelt P, Elliott HL, Springer TA
  TITLE     Coordinated integrin activation by actin-dependent force during 
            T-cell migration.
  JOURNAL   Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119.
  PUBMED    27721490
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-10"; volume: "7:13119. doi"; pages: " 
            10.1038/ncomms13119"
COMMENT     Sequence Label: pcDNA3.1/Hygro(-)-B2-cT
FEATURES             Location/Qualifiers
     source          1..9357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2998..3705
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3796..3798,3799..3801,3802..4515)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3841..3862)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4102..4121)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4449..4470
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4545..4562)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4551..4775
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4821..5249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4908..4927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5118..5139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5258..5278)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5263..5592
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5443..5578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5505..5524
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5641..6666
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5641..6666
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6796..6917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6833..6852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6887..6906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6966..6982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6966..6982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6979..7001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6990..7006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7014..7020,7021..7038,7039..7044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7059..7080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7197..7214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7368..7956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7448..7467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8127..8918,8919..8987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8750..8769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8988..9092)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9167..9186)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgcca ccatgctggg cctgcgcccc ccactgctcg ccctggtggg gctgctctcc
      721 ctcgggtgcg tcctctctca ggagtgcacg aagttcaagg tcagcagctg ccgggaatgc
      781 atcgagtcgg ggcccggctg cacctggtgc cagaagctga acttcacagg gccgggggat
      841 cctgactcca ttcgctgcga cacccggcca cagctgctca tgaggggctg tgcggctgac
      901 gacatcatgg accccacaag cctcgctgaa acccaggaag accacaatgg gggccagaag
      961 cagctgtccc cacaaaaagt gacgctttac ctgcgaccag gccaggcagc agcgttcaac
     1021 gtgaccttcc ggcgggccaa gggctacccc atcgacctgt actatctgat ggacctctcc
     1081 tactccatgc ttgatgacct caggaatgtc aagaagctag gtggcgacct gctccgggcc
     1141 ctcaacgaga tcaccgagtc cggccgcatt ggcttcgggt ccttcgtgga caagaccgtg
     1201 ctgccgttcg tgaacacgca ccctgataag ctgcgaaacc catgccccaa caaggagaaa
     1261 gagtgccagc ccccgtttgc cttcaggcac gtgctgaagc tgaccaacaa ctccaaccag
     1321 tttcagaccg aggtcgggaa gcagctgatt tccggaaacc tggatgcacc cgagggtggg
     1381 ctggacgcca tgatgcaggt cgccgcctgc ccggaggaaa tcggctggcg caacgtcacg
     1441 cggctgctgg tgtttgccac tgatgacggc ttccatttcg cgggcgacgg aaagctgggc
     1501 gccatcctga cccccaacga cggccgctgt cacctggagg acaacttgta caagaggagc
     1561 aacgaattcg actacccatc ggtgggccag ctggcgcaca agctggctga aaacaacatc
     1621 cagcccatct tcgcggtgac cagtaggatg gtgaagacct acgagaaact caccgagatc
     1681 atccccaagt cagccgtggg ggagctgtct gaggactcca gcaatgtggt ccatctcatt
     1741 aagaatgctt acaataaact ctcctccagg gtcttcctgg atcacaacgc cctccccgac
     1801 accctgaaag tcacctacga ctccttctgc agcaatggag tgacgcacag gaaccagccc
     1861 agaggtgact gtgatggcgt gcagatcaat gtcccgatca ccttccaggt gaaggtcacg
     1921 gccacagagt gcatccagga gcagtcgttt gtcatccggg cgctgggctt cacggacata
     1981 gtgaccgtgc aggttcttcc ccagtgtgag tgccggtgcc gggaccagag cagagaccgc
     2041 agcctctgcc atggcaaggg cttcttggag tgcggcatct gcaggtgtga cactggctac
     2101 attgggaaaa actgtgagtg ccagacacag ggccggagca gccaggagct ggaaggaagc
     2161 tgccggaagg acaacaactc catcatctgc tcagggctgg gggactgtgt ctgcgggcag
     2221 tgcctgtgcc acaccagcga cgtccccggc aagctgatat acgggcagta ctgcgagtgt
     2281 gacaccatca actgtgagcg ctacaacggc caggtctgcg gcggcccggg gagggggctc
     2341 tgcttctgcg ggaagtgccg ctgccacccg ggctttgagg gctcagcgtg ccagtgcgag
     2401 aggaccactg agggctgcct gaacccgcgg cgtgttgagt gtagtggtcg tggccggtgc
     2461 cgctgcaacg tatgcgagtg ccattcaggc taccagctgc ctctgtgcca ggagtgcccc
     2521 ggctgcccct caccctgtgg caagtacatc tcctgcgccg agtgcctgaa gttcgaaaag
     2581 ggcccctttg ggaagaactg cagcgcggcg tgtccgggcc tgcagctgtc gaacaacccc
     2641 gtgaagggca ggacctgcaa ggagagggac tcagagggct gctgggtggc ctacacgctg
     2701 gagcagcagg acgggatgga ccgctacctc atctatgtgg atgagagccg agagtgtgtg
     2761 gcaggcccca acatcgccgc catcgtcggg ggcaccgtgg caggcatcgt gctgatcggc
     2821 attctcctgc tggtcatctg gaaggctctg atccacctga gcgacctccg ggagtacagg
     2881 cgctttgaga aggagaagct caagtcccag tggaacaatg ataatcccct tttcaagagc
     2941 gccaccacga cggtcatgaa ccccaagttt gctgagagtg gtggacctgt ggcaactatg
     3001 gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg
     3061 aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag
     3121 ccctacgacg gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc
     3181 tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc
     3241 gacgacatcc ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc
     3301 atgaccttcg aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac
     3361 tccttcatct acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg
     3421 cagaagaaga ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg
     3481 ctgaagggcg acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac
     3541 ttcaagacca tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac
     3601 caccgcatcg agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc
     3661 gccgtggccc gcaactccac cgacggcatg gacgagctgt acaaggggcc aggtggtgca
     3721 gggccaggtg gtgcagggcc cggtggtgca ggtccaggtg gtgcaggtcc aggtggtgca
     3781 ggtccaggtg gtgctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3841 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3901 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     3961 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     4021 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4081 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4141 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4201 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     4261 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     4321 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4381 ctgcccgaca accactacct gagctaccag tccaaactga gcaaagaccc caacgagaag
     4441 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4501 gagctgtaca agtagtaaaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc
     4561 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4621 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4681 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4741 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     4801 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4861 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4921 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4981 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5041 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5101 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5161 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5221 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     5281 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5341 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5401 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5461 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5521 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5581 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     5641 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     5701 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     5761 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     5821 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     5881 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     5941 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     6001 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6061 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6121 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     6181 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     6241 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     6301 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6361 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6421 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     6481 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     6541 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     6601 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     6661 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9121 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9181 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9241 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9301 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
