LOCUS       sequence-201138-        5166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Casp1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Shi G, Abbott KN, Wu W, Salter RD, Keyel PA
  TITLE     Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion.
  JOURNAL   Front Immunol. 2017 May 8;8:522. doi: 10.3389/fimmu.2017.00522. 
            eCollection 2017.
  PUBMED    28533778
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2017-05-8"; volume: "8:522. doi"; pages: " 
            10.3389/fimmu.2017.00522. eCollection 2017"
COMMENT     Sequence Label: Casp1
FEATURES             Location/Qualifiers
     source          1..5166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      558..567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1894..2015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1931..1950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1985..2004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2022..2477)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2159..2180)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2371..2390
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2504..2608
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2610..2967
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2610..2801
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2633..2653)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2818..2953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3002..3796
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3002..3796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3056..3075)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3666..3685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3984..4003)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4028..4075
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4404..4992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4893..4912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagcc accatggctg acaagatcct gagggcaaag aggaagcaat
      601 ttatcaactc agtgagtata gggacaataa atggattgtt ggatgaactt ttagagaaga
      661 gagtgctgaa tcaggaagaa atggataaaa taaaacttgc aaacattact gctatggaca
      721 aggcacggga cctatgtgat catgtctcta aaaaagggcc ccaggcaagc caaatcttta
      781 tcacttacat ttgtaatgaa gactgctacc tggcaggaat tctggagctt caatcagctc
      841 catcagctga aacatttgtt gctacagaag attctaaagg aggacatcct tcatcctcag
      901 aaacaaagga agaacagaac aaagaagatg gcacatttcc aggactgact gggaccctca
      961 agttttgccc tttagaaaaa gcccagaagt tatggaaaga aaatccttca gagatttatc
     1021 caataatgaa tacaaccact cgtacacgtc ttgccctcat tatctgcaac acagagtttc
     1081 aacatctttc tccgagggtt ggagctcaag ttgacctcag agaaatgaag ttgctgctgg
     1141 aggatctggg gtataccgtg aaagtgaaag aaaatctcac agctctggag atggtgaaag
     1201 aggtgaaaga atttgctgcc tgcccagagc acaagacttc tgacagtact ttccttgtat
     1261 tcatgtctca tggtatccag gagggaatat gtgggaccac atactctaat gaagtttcag
     1321 atattttaaa ggttgacaca atctttcaga tgatgaacac tttgaagtgc ccaagcttga
     1381 aagacaagcc caaggtgatc attattcagg catgccgtgg agagaaacaa ggagtggtgt
     1441 tgttaaaaga ttcagtaaga gactctgaag aggatttctt aacggatgca atttttgaag
     1501 atgatggcat taagaaggcc catatagaga aagattttat tgctttctgc tcttcaacac
     1561 cagataatgt gtcttggaga catcctgtca ggggctcact tttcattgag tcactcatca
     1621 aacacatgaa agaatatgcc tggtcttgtg acttggagga cattttcaga aaggttcgat
     1681 tttcatttga acaaccagaa tttaggctac agatgcccac tgctgatagg gtgaccctga
     1741 caaaacgttt ctacctcttc ccgggacatt aagcggccgc gactctagat cataatcagc
     1801 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1861 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1981 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     2041 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2101 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2161 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2221 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2281 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2341 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2401 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2461 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2521 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2581 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     2641 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2701 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     2761 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2821 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2881 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2941 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     3001 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3061 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3121 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3181 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3241 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3301 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3361 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3421 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3481 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     3541 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3601 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3661 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3721 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3781 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     3841 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3901 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     3961 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4021 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4081 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     4141 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     4201 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     4261 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4321 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5041 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5101 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5161 gttccg
//
