LOCUS       sequence-201151-       20465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of ekivR, luc, esyn1 using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 20465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS37.13
FEATURES             Location/Qualifiers
     source          1..20465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          341..456
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             1856..1912
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3660..3716
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(15796..15815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15797..15815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15825..15842)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15825..15841)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15834..15856)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15983..16438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16070..16089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16280..16301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        16464..16568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16569..16637,16638..17429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16787..16806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17600..18188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18089..18108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18342..18359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    18476..18497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18512..18517,18518..18535,18536..18542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18550..18566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18555..18577
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18574..18590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18574..18590
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     18609..18629
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        18611..18629
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     18658..18674
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     18659..18675
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          19239..19354
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(19372..19989,19990..19995,19996..20031,20032..20034,
                     20035..20070,20071..20073,20074..20109,20110..20118)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcc ccatcttcag
      301 tatattcatc ttcccatcca agaaccttta tttcccctaa gtaagtactt tgctacatcc
      361 atactccatc cttcccatcc cttattcctt tgaacctttc agttcgagct ttcccacttc
      421 atcgcagctt gactaacagc taccccgctt gagcagacat caccgtttaa acaccatgga
      481 aaagcctgtg gcctcacaag tccaccccag ctggctaaca gctcttctac ctgaatccag
      541 aatacccccc aagctgtttg cctggtcctc agccaacatc cgcccagcaa gctcctccaa
      601 gctcggaggc tctgccacca acatccacca tgcagagtgt tcacatccaa ccccaaactc
      661 cacagcaagc tctgagcagc gcatcttcat cgttggtccc ggcaacatag gtaggctcta
      721 cgcaagccac atggtccgcc gcccaaatcc cctccccatc actctcgtcg tccccagaaa
      781 ggaactccta tcgcaatggg caggctgtga aggcgtgggc cttatagacc cgggtcgcgg
      841 caaggtcctc aaaagcaagc gcttcaccgt cgagtggtgg acggagacta gaccacagta
      901 tggtcctgtg agagaggtgg cagacggaaa gaagctgcgc aacgtgttca tatccacaaa
      961 ggcatccgct gggctggccg aggcagatcg tcttcgtcgg tatttgggca agtgtagttc
     1021 tgtcgtcttt gcgcagaacg gagtatgcaa gctgtggcct cctcatgggc cgttatacgt
     1081 cgccggtcga taccctcctg gtgatgctcc taccttctca tcctgcgtcg tgaatcatgg
     1141 cgttgcatca gcggggcctt ttctgagcat tcacgcagct ccggcagatg catccatcgg
     1201 acctgtcctc tggccttccc atccgatgcc gcacaagaaa cccctgaacg acttcttcac
     1261 aagatacatt gctactacac ccttcctaaa cacaaagcaa gtttcctctg gtgagctctg
     1321 gcttattcag ctggagaaac ttgttaccaa tgccgccatc aaccccctca cagctttgct
     1381 acgctgcaaa acgggacagc tgttcacaac ccatgacccc acagaccctc ttacgcgcgt
     1441 gctggacagg ttgctatggc aggccagcgc cgtgattcag ggtcttatca accacgacgc
     1501 gagtgtcgac atcgtcgctt cctacgccga gcacctccac cagaccgaac caagcagcga
     1561 ctcaagcctt gtcgcagtac ggaccaagtt aacagagcgg ttctcccagc ctaccttgag
     1621 agcgaagcta ttcgcctttg gtcgcaagat atacgagcat cgcagctcga tgcttcagga
     1681 tgttgaggcc ggcaggaaaa cggagataag agatttcaac gggtgggttg tggatatggc
     1741 gagttttctg gggacggggc tgaatgtgag cgtgcatcag agtttgattg tgctgattga
     1801 acgcggtgag gttttgaaca aggatgagtt ggctaggagg acattaggct ccggcgccac
     1861 caacttctcc ctcctgaagc aggccggtga cgtcgaggag aaccccggcc ccatggagga
     1921 cgccaagaac atcaagaagg gccccgcccc cttctacccc ctcgaggacg gcaccgccgg
     1981 cgagcagctc cacaaggcca tgaagcgcta cgccctcgtc cccggcacca tcgccttcgt
     2041 aggtttcctc cagctctcgc ctccagcacc cgaggcacat ctcgggcatc ttcacaacaa
     2101 cagacactga catctcattc tcacagaccg acgcccacat cgaggtcaac atcacctacg
     2161 ccgagtactt cgagatgtcc gtccgcctcg ccgaggccat gaagcgctac ggcctcaaca
     2221 ccaaccaccg catcgtcgtc tgctccgaga actccctcca gttcttcatg cccgtcctcg
     2281 gcgccctctt catcggcgtc gccgtcgccc ccgccaacga catctacaac gagcgcgagc
     2341 tcctcaactc catgaacatc tcccagccca ccgtcgtctt cgtctccaag aagggcctcc
     2401 agaagatcct caacgtccag aagaagctcc ccatcatcca gaagatcatc atcatggact
     2461 ccaagaccga ctaccagggc ttccagtcca tgtacacctt cgtcacctcc cacctccccc
     2521 ccggcttcaa cgagtacgac ttcgtccccg agtccttcga ccgcgacaag accatcgccc
     2581 tcatcatgaa ctcctccggc tccaccggcc tccccaaggg cgtcgccctc ccccaccgca
     2641 ccgcctgcgt ccgcttctcc cacgcccgcg accccatctt cggcaaccag atcatccccg
     2701 acaccgccat cctctccgtc gtccccttcc accacggctt cggcatgttc accaccctcg
     2761 gctacctcat ctgcggcttc cgcgtcgtcc tcatgtaccg cttcgaggag gagctcttcc
     2821 tccgctccct ccaggactac aagatccagt ccgccctcct cgtccccacc ctcttctcct
     2881 tcttcgccaa gtccaccctc atcgacaagt acgacctctc caacctccac gagatcgcct
     2941 ccggcggcgc ccccctctcc aaggaggtcg gcgaggccgt cgccaagcgc ttccacctcc
     3001 ccggcatccg ccagggctac ggcctcaccg agaccacctc cgccatcctc atcacccccg
     3061 agggcgacga caagcccggc gccgtcggca aggtcgtccc cttcttcgag gccaaggtcg
     3121 tcgacctcga caccggcaag accctcggcg tcaaccagcg cggcgagctc tgcgtccgcg
     3181 gccccatgat catgtccggc tacgtcaaca accccgaggc caccaacgcc ctcatcgaca
     3241 aggacggctg gctccactcc ggcgacatcg cctactggga cgaggacgag cacttcttca
     3301 tcgtcgaccg cctcaagtcc ctcatcaagt acaagggcta ccaggtcgcc cccgccgagc
     3361 tcgagtccat cctcctccag caccccaaca tcttcgacgc cggcgtcgcc ggcctccccg
     3421 acgacgacgc cggcgagctc cccgccgccg tcgtcgtcct cgagcacggc aagaccatga
     3481 ccgagaagga gatcgtcgac tacgtcgcct cccaggtcac caccgccaag aagctccgcg
     3541 gcggcgtcgt cttcgtcgac gaggtcccca agggcctcac cggcaagctc gacgcccgca
     3601 agatccgcga gatcctcatc aaggccaaga agggcggcaa gtccaagctc ggctccggcg
     3661 ccaccaactt ctccctcctg aagcaggccg gtgacgtcga ggagaacccc ggccccatgt
     3721 cactccacac cccaagtgac ggtcaacaag acccagcgtt agcctccaaa accctctgtg
     3781 agcaaatctc gcgagccctc ggcctcggcc aggacaagat tgagaatatc ttcccaggga
     3841 cacccttcca gcgggacgtc atagactgcg ccgcagacga caagcagcgc gccgtgggcc
     3901 acgctgtctt tgaaattccc aaagatatag acgcggcccg cctggcggct gcgtggaagg
     3961 agaccgtgct tcacacccct gctctgcgga cctgcacgtt tacgtccaaa tctggggatg
     4021 tgcttcaggt ggtcctgaga gatagctttg tcttttcgtg gatgtctggg ccgtctgtgg
     4081 atcttaagga ggcggttgtg caagacgagg ctgctgctgc tttggctggc ccgcgctgta
     4141 accgcttcgt ccttcttgag gaccctgata caaaggaacg tcagctcatc tggacgttca
     4201 gccatgctct cgttgatagt accttccagg aacgtatcct tcgacgagtc ctcaaagctt
     4261 acaaggatgc caatgacgaa catcctcgcc agttcgagac accagattct tcccaggcca
     4321 cgcccgagga ggatttacag cccaatccat ccaagatgct caagatccct caagccgcag
     4381 atatggaccg tgctgttgag ttttggaagg atcatctaag tggcctgaat gcttctgcct
     4441 tcccgcattt gtcctctcat ctgtctatgc cacatccaga tgccaaggca gagcatagaa
     4501 tctcctactc gtcttcagca cagcaaaaaa tgtccagtgc tactatttgc cgaactgccc
     4561 tggcaattct gttatctcgt tatacgcact ctcctgaagc actctttggc attgtaactg
     4621 agcaaactcc tctactggag gagcaactca tgcttgatgg cccaacacgt acagtcgtac
     4681 caatccgcgt atcctgtgct tctgaacagt cagtgtcaga tatcatgagc acgattgact
     4741 cctacgatca gaccatgcgg cagtttgccc acgctggtct tcgcaacatc gccagtgctg
     4801 gagacgatga atctgccgcc tgcgggttcc agaccgttct cttggtctca gatggagacg
     4861 cccagccagc atctacttgg gaaattctca agaaaactga agagcctgag ggtttcatcc
     4921 cctgtacgaa ccgtgctctg cttctaagtt gccagatgac cagctctgga gcacacctaa
     4981 ccgccagata cgaccagagt atcatcgacg ctgagcaaat ggcccgactc ctgcgacaac
     5041 taggccatct gatccaaaat cttcaaacct ccacagatct acccgtggaa aaggtggaca
     5101 tgatgacgca agaagactgg ttagagattg agaggtggaa ctcagactcc atcgacgcac
     5161 aagacacgct tatccacagc gagatgttga agtggacttc tcagtcgcct aacaaagctg
     5221 ccgtcgccgc gtgggatgga gagtggacgt atgctgagct ggacaatgta tcttcgcgcc
     5281 tggctcagca tatcaactct attgacctgg gcaaggagca cgcgatagta cccatctact
     5341 ttgagaaatc gaaatgggtc gttgcttcga tgctggctgt gctcaaggct ggccatgcgt
     5401 ttactctcat tgaccccagt gatccaccag ctcgaacagc acaggtcgtg cagcaaacct
     5461 ctgcgacagt tgctcttact tccaagctcc atcgcgagac tgttcaatca actgtcgggc
     5521 gctgcattgt tgtcgacgaa gagtttgtca aatccctccc ccagtccagc gaactctcag
     5581 cgtctgtaaa ggcacatgac ctggcatatg tcatcttcac ctcgggcagc acaggcattc
     5641 caaagggaat catgattgag caccgctcat tttcatcctg cgccatcaaa ttcggtcccg
     5701 cgcttggaat aacctccgac acacgcgctc ttcagtttgg atctcatgcc tttggcgcgt
     5761 gtatcctcga gatcatgacg acgctcatcc acggaggctg tgtctgtatc ccctctgacg
     5821 atgaccgcat gaacaacgtc cttgagttca tcaaccggac taacgtcaac tgggtcatgg
     5881 caacgccttc gtacatgggg acattccagc cagaggttgt tcctggtttg aagaccttgg
     5941 tattggtagg tgagcagatg tctgcttctg tgaatgaggt ctgggctcct cgtgttcaac
     6001 tcctgaacgg ttacggacag agcgagagtt cttctatctg ctgcgtagcc aagatcagcc
     6061 ctggttcatc tgagccaaac aatattggcc acgcagtagg cgcacattct tggatcgttg
     6121 accctgagga tcccaatcgc ttggctccca tcggtgccgt tggcgagctg gtcatcgaga
     6181 gtgcaggtat cgcacgcgat tacatcgttg cgccaactca ggataagtct cctttcatta
     6241 agactgctcc tacgtggtat ccggccaagc agctcccgga cggcttcaag atctacagaa
     6301 caggcgatct tgcatgctac gcatctgatg gcagtatagt gtgccttggc cgtatggact
     6361 cccaggtcaa gatcagagga cagcgtgttg agctgggtgc agtggagact catcttcgac
     6421 agcagatgcc tgatgatatg accatcgttg ttgaagctgt caagttctct gattcctcca
     6481 gtactacagt tttgacagcc ttcttgattg gtgcagggga gaagaacagt catatcttgg
     6541 accagcgtgc tacaagggag atcaatgcca agatggagca agttcttcct cgccattcca
     6601 tccccgcgtt ctacatctct atgaacaacc tcccccaaac agccactggt aaggtcgatc
     6661 gacgaaaact tcgtatcatg ggtagcaaga tactgagcca aaagacgcat tccaccccat
     6721 ctcagcagag tcaggccgcc atctcatctg gaaccgacac agagaccaag ctggagagca
     6781 tctggatcac aagcttggac ctcgagcccg gctctgcaaa catgtcagct acattcttcg
     6841 agatgggcgg aaactccatc atcgcaatca agatggtcaa catggcgcgc tccaatggca
     6901 tagagcttaa ggtctcggac atttaccaga accccacgct cgctggtctc aaggctattg
     6961 tcattggtac ttcgctgcca tacagcctta ttcccaaggt tacccgccaa ggccctgtta
     7021 gcgagcagtc ttatgcgcaa aacagaatgt ggttcctgga tcagttgtct gagggtgctt
     7081 catggtatct gattcctttc gctgtgcgca tgcgaggtcc ggtggatgtt gatgcgttga
     7141 cgcgtgcttt gctcgctctt gaacagcgtc atgagacact acgaactaca tttgagaacc
     7201 aagatggtgt tggagtccag atcatccatg atagactctc caaagagcta caagtcatcg
     7261 atgccttgga tggtgacgag ggtggtctca agacactcta caaagtagag accaccacat
     7321 tcgacatcac atccgaagca ggctggagct caaccctcat ccgcctcggc aaagacgatc
     7381 acattctgtc tatcgtcatg caccacatca tctccgacgg ctggtccatc gacgttctcc
     7441 gccgcgaact catccaactc tacgccgccg ctctgcaggg caaggatcct tcctccgcac
     7501 taacccccct acccatccag tacagcgact tcgccgtgtg gcagaagcag gaggcccaag
     7561 cagctgagca cgagaggcag ctccagtact ggaagaagca actcgcagat agttcacctg
     7621 ccaagatccc taccgacttc ccccgtccag atctcctgtc cggtgacgca ggcgttgtgc
     7681 ccgttgccat cgacggcgag ctgtatcaga aactaagggg cttctgcaac aagcacaaca
     7741 gcactgcctt ctccatcctg ctcgctgctt tccgcgcggc gcattaccgt ctcacagccg
     7801 ttgacgacgc cgtgatcggc atccccattg caaaccgtaa ccgctgggag ctggagaaca
     7861 tgattggttt ctttgtcaac acgcagtgta tgcgcatcgc cgttgacgag acggatacat
     7921 ttgagagtct ggtgcgccag gtcagatcta ccactacagc tgcgtttgcg cacgaggatg
     7981 tccccttcga gcgtgtcgtt tcagcgcttc agcctggcca tagagatctc tcgcgaacac
     8041 cgctggcaca gataatgttt gctgttcact cgcagaagga ccttggacgt ttcgagctgg
     8101 agggtatcca gtctgagcct atcgccagca aggcctacac cagattcgat gtcgagttcc
     8161 atctgttcca acaggcagac ggactgaagg gcagttgcaa ctttgccaca gatctcttca
     8221 agcccgaaac tatccagaat gttgtcagcg tgtttttcca gattctacgc catggccttg
     8281 accagcctga gacgtgtatc tcggttcttc cactgactga tggagtcgag gagcttcgca
     8341 ggttggatct gctggaaatc aagaggacta actaccctcg cgattcgagc gtggtagatg
     8401 tcttccgcga acaagctgcc gccaacccag aggttatcgc tgttaccgac tcatcttctc
     8461 gtctgactta tgcagagctg gacaataagt ctgagctgct ctcacgatgg cttcgacgac
     8521 gtaacttgac gccagagacg ctggtcagtg ttcttgctcc ccggtcttgc gagactatcg
     8581 ttgcctatgt tggtatcctc aaggcgaacc tggcgtatct tcctcttgac gtgaggtccc
     8641 cggtgactcg tatgaaggac atcttgtcga gcgtgtctgg aaacaccata gttcttatgg
     8701 gctctggggt agaggatcct ggctttgact taccgcaact agagctcgta cgcatcaccg
     8761 acactttcga tgagaccatc gaggacgtgc aagactctcc ccaaccgtct gccacaagcc
     8821 tcgcctacgt cgtcttcaca tccggttcaa ctggtaaacc caagggcgtc atgatcgagc
     8881 accgggccat tgtgcgtctc gtcaagagtg acaactttcc tggcttcccc tcccccgctc
     8941 gcatgtcaaa tgtcttcaac cctgccttcg acggagccat ctgggagatc aactggatgc
     9001 tcctgaacgg cggaacagtc gtctgcatcg actacctgac caccctggac ggcaaagagc
     9061 tcgctgctgt gttcgccaaa gagcgcgtca acgccgcctt cttcgcacct gcgatgctca
     9121 agctttacct cgttgatgcg cgcgaggctt tgaagaatct tgacttcctt attgttggag
     9181 gtgagaggtt cgataccaag gaagccgtgg aggccatgcc gcttgtgaga ggcaagattg
     9241 ccaatatcta tggtccgact gaggctggaa taatcagcac gtgctataac atccccaagg
     9301 atgaggctta caccaatggt gttcccattg gtggaagtat ctacaactct ggtgcctacg
     9361 tcatggatcc taaccagcaa cttgtcggcc ttggcgtcat gggagagctt gttgttaccg
     9421 gagacggtgt tggtcgaggc tacactaacc ccgagctcaa caagaaccgc ttcatcgaca
     9481 tcaccatcga gggcaagact ttcaaggctt accgtactgg tgaccggatg cgtgcacgag
     9541 taggcgacgg tctccttgag ttcttcggcc gcatggacaa ccagttcaag atccgcggca
     9601 accgtatcga agcaggggaa gttgagtctg ccatgctcag cctcaagaat gtccttaacg
     9661 ccgccattgt cgtccgcggg ggcggagaag atgaagggcc actcgagatg gtcggattca
     9721 tcgtcgcgga cgacaagaat gataccacgg aggaggaaga gacaggcaac caagttgagg
     9781 gctggcagga ccatttcgag agtggtatgt actcggatat cagcaccgcc gtggaccaat
     9841 ctgccattgg aaacgacttt aagggctgga cttctatgta cgatggtaag gatatcgaca
     9901 agggcgagat gcaggagtgg ttggacgacg ctattcacac cctgcataac ggccagatcc
     9961 ctcgcgatgt cctcgagatc ggtaccggta gtggtatgat cctgttcaac ctcaacccgg
    10021 gcctcaacag ctacgttggt cttgatccat ccaagtcagc agtcgagttc gtcaacagag
    10081 ccgtcgagtc ctctcccaag ttcgcaggaa aagccaaggt ccacgtcggc atggccacag
    10141 acgtcaacaa actcggcgaa gtacaccccg atctcgtggt cttcaactcc gttgttcaat
    10201 acttccccac acccgagtat ctcgccgagg tcatcgatgg cctcattgcc atccccagcg
    10261 tcaagcgcat cttccttggc gatatcagat catatgctac caacggacac ttcctcgccg
    10321 cacgcgccat ccacacgctc ggcaccaata acaacgccac caaggatagg gtgcgccaga
    10381 agatccagga gctggaggat cgagaggaag agtttctcgt tgagcctgcc ttcttcacca
    10441 ctctgaagga gcgacgtcca gatgttgtca agcatgttga gatcatcccc aagaacatga
    10501 aggccaccaa cgaactcagc gcctatcgct acacggctgt tgtgcatctg cgggatgaaa
    10561 cggacgaacc tgtgtatcat attgagaagg atagctgggt tgactttgag gcgaagcaga
    10621 tggacaagac ggctcttctt gaccacctgc gcctctccaa ggatgctatg agtgtggcgg
    10681 ttagcaacat cacctacgcc cacactgcct ttgaacgtcg tatcgttgag tctctcgatg
    10741 aggatagcaa ggatgacacc aagggtacac tcgatggtgc agcctggctc tcagcagttc
    10801 gctccgaagc cgaaaaccgc gcctcactca ccgtccccga catcctggag atcgccaaag
    10861 aggctggttt ccgagttgaa gtcagcgctg ctcgccagtg gtcccaaagt ggtgctttag
    10921 acgcagtctt ccaccacttt ccaccctcca gcactgaccg cactctaatc cagttcccca
    10981 cggacaacga gcttcgatca tcactcaccc tcgccaaccg ccctctccag aagctgcagc
    11041 gccgtcgtgc cgctctgcaa gtccgcgaga agctccagac gctggtcccg tcttacatgg
    11101 ttcctccgaa tatcgtggtg ctggacacga tgcctctcaa tactaacggc aagatcgaca
    11161 gaaaggagct tacgcgtaga gcacgaacac tgccgaagca gcagactgcg gcgcctgtgc
    11221 cggacttccc tatctctgat atcgagatca cgctgtgcga ggaggcaact gaggtctttg
    11281 gaatgaaggt tgaaatcagc gatcacttct tccagctcgg cggtcactct ctcctcgcta
    11341 cgaaactcat ttctcgcatc cagcaccgtc tccatgtgcg ggttactgtg aaggacgtat
    11401 tcgacagccc tgtctttgcc gatctggcag tcatcatccg tcaaggactt gctatgcaga
    11461 accctgttgc tgaaggacag gacaagcaag gctggtcctc gagagttgcc cctcgtacag
    11521 aagtcgagaa gatgctgtgc gaggagttcg cagctggtct tggtgtcccg gttggtatca
    11581 ctgacaactt cttcgatctc ggtggtcact cgctcatggc tactaagcta gctgtgcgaa
    11641 ttggccgtcg ccttgatacc gccatcacag tcaaggacat cttcgactac cctgtgcttt
    11701 tccaattggc gaagaagttg gagtcttcgc attccaagag ctacgaggag tctggcgacg
    11761 atatccagat ggccgattac actgcattcc agctcctcga tctggaagac ccccaagact
    11821 ttgttcagtc ccagattcgg cctcaactgg actcctgcta cggcaccatc caggatgtct
    11881 acccgtctac gcagatgcaa aaggccttcc tcttcgatcc cacgaccggc gagccccgag
    11941 gtcttgtgcc tttctatatc gacttcccca gcaatgcaga tgccgagacc ctcaccaagg
    12001 ctatcggagc tctagttgac aagctcgata tgttcagaac tgtcttcctg gaggccgcag
    12061 gcgatctgta ccaagttgtc gttgagcacc tcaacttgcc tattgagacc atcgagactg
    12121 agaagaacgt caacactgca accggtgact acctggatgt tcatggaaag gaccctgtcc
    12181 gtctaggcca cccgtgcatc caattcgcca tcctgaagac tgcctcctct gtacgtgttc
    12241 tcctgcgaat gtcccacgct ctgtatgatg gtttgagttt tgagtacatc gtccgtggtc
    12301 tccacgttct ctacagcggt agaaacctcc ccccaccaac acagtttgcg cgatacatgc
    12361 agtatgctgc acacagtcgt gaagaaggtt atcccttctg gcgcgaggtt ctgcagaacg
    12421 cgcccatgac agttctacac gacaccaata acggtatgtc tgagcaagag atgccagcct
    12481 ccaaggcggt acacctgtca gaggtcgtca acgttccagc gcaagctatt cgaaacagta
    12541 ccaacaccca agcgaccgtc ttcaacaccg cctgtgccct tgtcctagcc aaggaatccg
    12601 gctcacagga tgtcgtcttc ggccgtattg tctctggtcg acaaggtcta ccagtcgtct
    12661 ggcaggacat catcggcccc tgcacaaacg ccgtgcccgt ccacgcacgc gtcgacgatg
    12721 gaaaccccca acgcatcatc cgcgacctac gcgaccaata cctccgcact ctgcccttcg
    12781 aatcgcttgg cttcgaggaa atcaagcgta actgcacgga ctggcccgaa gaattgacca
    12841 acttctctgt ctgcgtgacg taccacaact tcgagtacca ccccgagagc gaagttgaca
    12901 accaaaaggt tgagatggga gttttggcaa agtatgttga gttgagtgaa aacgagccgc
    12961 tgtacgatct tgctattgcg ggagaggttg aggcggatgg agttaacctg aaggtcactg
    13021 ttgttgcaaa ggcaaggctt tacaatgagg cgaggattag acatgtgctt gaggaagttt
    13081 gcaaaacttt caatggtttg aacgaggctt tgtaagttta aacagtagat gccgaccggg
    13141 atccacttaa cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg
    13201 gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt
    13261 catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa
    13321 taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc attaatgcat
    13381 tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa tagcttagca
    13441 gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc aagagaccta
    13501 cgagactgag gaatccgctc ttggctccac gcgggcgcgc ctcggtcgct cactgttcct
    13561 ttacggatat gtgtactccg gcaagccgcg gggtcgcggt cgtttgtacg gcagcttcta
    13621 cttgcttgca cagggagctc tctggggctt gacgtctttt ggagttgcga gggagttgat
    13681 ttcctacttc taagtttgga ctgaatccgt ggtgtgattg aggtgattgg cgatgtttgg
    13741 ctataccagc tatatgtaat aatctctact gtatactact attcaacgca ttttactatg
    13801 cgtgctgcta gggtcggcaa tgacaatggc aatctgactg acgtggtcta tttctccatg
    13861 tgcagcaggg aatacgagct ccaatggacc tcgggagtgg cacagtcaat ggcaaggaaa
    13921 ctccgccttt gcaggtgtgg ctgaacccca cgggtcggag gcggagcaat ccacccccga
    13981 tgtggctggt gcgtggaggg gctcgcgatg attttactga gcttgctttt cttgtcgaca
    14041 ttgaacattg tccttggtct tccttcagat ttaagggtca gtcactgcta catttctcag
    14101 tagtatccgc gcacgtctct ggatttacga atcagggtcc accagtcgaa acttcgaact
    14161 actctcatta tacaatcctc tttccattcc cgcattaacc cctccatcaa caccatgtcc
    14221 tccaagtcgc aattgaccta cactgcccgt gccagcaagc atcccaatgc tctggcgaag
    14281 aggctgttcg agattgccga ggccaagaag accaatgtga ctgtctcggc tgacgttacc
    14341 accactaagg agctactaga tcttgctgac cgtaggccga cccgctactc tgcctgatta
    14401 tgctgcatgc aaacttatta acggtgatac cggactgcag gtctcggtcc ctacattgcc
    14461 gtgatcaaaa cccacatcga tatcctctct gatttcagca acgagaccat tgagggactt
    14521 aaggctctcg cgcagaagca caactttctc atcttcgagg accgcaagtt cattgacatc
    14581 ggcaacacgg tccagaagca ataccacggc ggtaccctcc gtatctcgga atgggcccac
    14641 atcatcaact gcagcattct ccctggtgag ggtatcgtcg aggctctcgc tcagacggcg
    14701 tctgcaccgg acttcgccta cggccccgaa cgcggtctgt tgatcttggc agagatgacc
    14761 tctaagggct ccttggctac cggccagtac actacttcct cggtcgatta tgcccggaaa
    14821 tacaagaact tcgttatggg attcgtgtcg acgcgcgcgt tgggtgaggt gcagtcggaa
    14881 gtcagctctc cttcggatga ggaggacttt gtggtcttca cgactggtgt gaacatttct
    14941 tccaagggag ataagcttgg tcagcagtac cagacgcccg gatcggctat cggccggggt
    15001 gctgacttca ttatcgcggg tcgcggtatc tacgccgcgc cggatcgatc cggtgcaggc
    15061 tgcgcaacag tatcagaagg aggggtggga agcctacctg gcccgtgtcg gcggaaacta
    15121 atactataaa aggaggatcg aagttctgat ggttatgaat gatatagaaa tgcaacttgc
    15181 cgcaacggat acggaagcgg aaacggacca atgtcgagca cgggtagtca gactgcggca
    15241 tcggatgtcc aaacggtatt gatcctgcag gctactatgg tgtggcacaa ggatcaatgc
    15301 ggtacgacga tttgatgcag ataagcaggc tgcgaagtag taactcttgc gtagagaaaa
    15361 tggcgacggg tgggctgata agggcggtga taagcttaat tgtcatcgca gataagcact
    15421 gctgtcttgc atccaagtca gcgtcagcag aaatacggga cttccgaaag tatatggcaa
    15481 aattaaagaa cttgactctc cagcaatgtt ttgccctgac cgtcgctaaa acgttactac
    15541 ccctataccc gtctgtttgt cccagcccga ggcattaggt ctgactgaca gcacggcgcc
    15601 atgcgggctt gggacgccat gtccgtcgcg tgataagggt tgatccatgc agctactatc
    15661 cttccatcgt tccattccca tccttgtcct atctccatcc ttgaaacttt actagtttag
    15721 ttggatgctc gagcttgctc tcggctactc cgtcggcgcg ccttgcggcc gccaccgcgg
    15781 tggagctcca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
    15841 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    15901 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    15961 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    16021 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    16081 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    16141 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    16201 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    16261 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    16321 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    16381 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    16441 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    16501 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    16561 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    16621 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    16681 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    16741 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    16801 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    16861 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    16921 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    16981 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    17041 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    17101 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    17161 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    17221 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    17281 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    17341 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    17401 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    17461 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    17521 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    17581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    17641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    17701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    17761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    17821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    17881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    17941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    18001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    18061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    18121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    18181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    18241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    18301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    18361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    18421 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    18481 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    18541 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    18601 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
    18661 aggtcgacgg tatcgataag cttcggagaa tatggagctt catcgaatca ccggcagtaa
    18721 gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc gaaatagcat gccattaacc
    18781 taggtacaga agtccaattg cttccgatct ggtaaaagat tcacgagata gtaccttctc
    18841 cgaagtaggt agagcgagta cccggcgcgt aagctcccta attggcccat ccggcatctg
    18901 tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg
    18961 ctcaggagct ggccagcggc gcagaccggg aacacaagct ggcagtcgac ccatccggtg
    19021 ctctgcactc gacctgctga ggtccctcag tccctggtag gcagctttgc cccgtctgtc
    19081 cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca gtccaacatt tgttgccata
    19141 ttttcctgct ctccccacca gctgctcttt tcttttctct ttcttttccc atcttcagta
    19201 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
    19261 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
    19321 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccgaattcac catgtctaga
    19381 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
    19441 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
    19501 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
    19561 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
    19621 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
    19681 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
    19741 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
    19801 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
    19861 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
    19921 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
    19981 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
    20041 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
    20101 gacatgctcc ccgggtaact aagtaaggat ccactagtac agcagaagaa tctctctccg
    20161 ctgttgcttc agtgtctgcc atgcattaac ttcatcctac tgtcctaccc gcagtaccca
    20221 ttcacatttg cggcagatac caggttgttt tctattccct cggttcttca gttcttcaga
    20281 tattacatta ctcgagaaat tggcggcgat gcaggagttt ggttggtttt aattgtttca
    20341 gtctccttga cctgatttgt atgacatgca atgtttcgac ggaagactat ctcggtgaat
    20401 atacgtcatg aatcatgcgg ccgcgcgtat cacgaggccc tttcgtcttc actcgagttt
    20461 accac
//
