LOCUS       sequence-201155-        2840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LI promoter module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Wu D, Schandry N, Lahaye T
  TITLE     A modular toolbox for Golden-Gate-based plasmid assembly streamlines
            generation of Ralstonia solanacearum species complex knockout 
            strains and multi-cassette complementation constructs.
  JOURNAL   Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632.
  PUBMED    29077245
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632."
COMMENT     Sequence Label: LI A-B pGala7
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(493..509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(493..509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(506..528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    517..533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(541..547,548..565,566..571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(724..741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(895..1483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(975..994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1598..1603,1604..1620,1621..1626)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1815..2348
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     2435..2453
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2491..2513)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2613..2632
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(2826..2840,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2840,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttcgagctcg gtacctcgcg aatgcatcta gatatcggat
       61 cccgggcccg tcgactgcag aggtacgggt ctcagcggaa tgtgacgtgt gcttgaggtg
      121 ctgccgtggc ggcagggcgc gcccggacgg cgtgcgcttc gtttgcccgt gtggcggctt
      181 cgtcaggccg gctgtacccg cccgggttgc cgtcattcgc gttgcagcgc gggcgatggg
      241 atgatcgcgc gtcctggccg gctgcgcgcg cgcccatgga atcccgcgac gcttgttcag
      301 cacaccctgg cccctgcacg cgcggcatcg cagcgcgttg ccggacagcg gcgcgagcgt
      361 tgtttgtcgc gcgctcaggc aggccggttt gcgcagggct gagccgttcg tttgccggca
      421 gcttctttcg tggtgccggc tggcgtcctg tctgcgagac ctctgcctgc atgcaagctt
      481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      541 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      601 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      661 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1141 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1321 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1501 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1561 gcgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg
     1621 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1681 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
     1741 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     1801 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
     1861 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
     1921 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcggc gacgtagcca
     1981 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
     2041 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
     2101 tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggag
     2161 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
     2221 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
     2281 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
     2341 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
     2401 tgtcacaacg ccgctcatga cattaaccta taaaaatagg cgtatcacga ggcccttttg
     2461 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagaaggt
     2521 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     2581 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2641 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2761 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2821 gttttcccag tcacgacgtt
//
