LOCUS       sequence-201224-        6275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter with ARHGAP29 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Qiao Y, Chen J, Lim YB, Finch-Edmondson ML, Seshachalam VP, Qin L, 
            Jiang T, Low BC, Singh H, Lim CT, Sudol M
  TITLE     YAP Regulates Actin Dynamics through ARHGAP29 and Promotes 
            Metastasis.
  JOURNAL   Cell Rep. 2017 May 23;19(8):1495-1502. doi: 
            10.1016/j.celrep.2017.04.075.
  PUBMED    28538170
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.04"; journalName: "Cell Rep"; date: 
            "2017-05-23- 23"; volume: "19"; issue: "8"; pages: "1495-1502"
COMMENT     Sequence Label: pGL3-BASIC-ARHGAP29 PROMOTER
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    71..119
                     /note="synthetic polyadenylation signal"
     misc_feature    133..224
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     173..192
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             1470..1481
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      1770..1779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1776..3428
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1842..1860)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3469..3590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3480..3499)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3534..3553
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3838..3855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4009..4597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4089..4108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4768..5559,5560..5628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5391..5410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5629..5733)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5760..6215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5847..5866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6057..6078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
       61 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      121 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
      181 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgat
      241 tgaatcgtag ttgcactgtt cagcctcccc ttggcctggg gactcctttg tctcctctta
      301 ttagtttgct tcagttcagg catcattggc ttccatggtg agagtccttg gcgcattttt
      361 ctttttcagc acgattagaa ggaattttgg tttttgaggt ttcctatttg tggcaggacc
      421 ttgttactat tattttgggg atttgagggg aaaatctgtg taagttgagt ccaaaaaatc
      481 cagtatggtg ccaaagtaac caaggagctg agttttctta tttatctttt ctaagtagca
      541 cctgaggagc caaatttgtt gtattttctt agcattgttt ttcctgtaag ggttcaaaga
      601 tatgtctatg taagatgtcc ctcttctctt tctttgtcaa tgagtttttc tcttgccccg
      661 ctaccctact tttggttctg tcatgctcac ttacttttct gtttgttaag tgggtagtct
      721 ctccttttat ttttcttcct tattttctct ttttgccttc acttatttct gccttccttg
      781 gttttcttcc cttttcctgt gtctgcacct cattttcttt ccagttttcc tcagcacatt
      841 ctcctgtgtc tttttctgtt tctcccgtgt gtggtcattt ttctcgcaga ccccagcgct
      901 acccacagtc tccctccctg tgttttttcc tctgtttctc tatgagttct ttctgaatgt
      961 tccagcactg aggtaagtgc catcgcttgt tccagcgagt ggtgtgatat agggactatg
     1021 tgctttctag ccctgcttgc tataattatt ccatctttct cagacttttt tttttcttct
     1081 cattatgaag tgagtttgaa agtgcagcta agaaagaaag gaccgggcac agtggctcat
     1141 gcttgtaatc ccagtacttt gggaggctga ggcgggtgga tcacctgagg tcgggaggtc
     1201 gggaattcga gaccagcttg gccaacatag cgaaaccctg cctctactaa aaatactaaa
     1261 attagcttgg cgtggtaatg ggtgcctata atcgcagcta ctcaggtggc tgaggtggga
     1321 gaatcaattg agcccgggag gcggaggttg cagtgagccg agatcacgcc attagactcg
     1381 tctgggcgac agagcgagac tccgtctcca aaaaaaaaaa aaaaaaaaaa aaanntacag
     1441 ataggcctaa gtggaaacta ttaccctcca tagagggcag aggggcagag tagatggctg
     1501 gttttgaggt agttcattct tcattcagac gtgtggcgcc cttccccagg ccctaacccc
     1561 tggaagaggg aggaaaaagg cgatccacag aagtgtctca taagaaatgt agctgctcat
     1621 ctggccatta gccgcatcta gtcctcaggt gacttttctc tgagtcgctc tgcctgtccc
     1681 ctgcaagcca aaagggcagc cgacgcagcc cgtgatgctg ggacagcgtc cggccgctga
     1741 aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaga
     1801 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
     1861 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
     1921 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
     1981 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
     2041 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
     2101 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
     2161 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
     2221 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
     2281 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
     2341 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
     2401 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
     2461 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
     2521 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     2581 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
     2641 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     2701 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
     2761 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
     2821 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     2881 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
     2941 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     3001 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
     3061 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
     3121 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg
     3181 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     3241 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     3301 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     3361 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
     3421 ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     3481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     3601 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     3661 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc
     3721 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     3781 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
     3841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4441 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5761 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5821 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5881 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5941 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6001 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6061 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6121 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6181 cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa
     6241 ctgttgggaa gggcgatcgg tgcgggcctc ttcgc
//
