LOCUS       sequence-201306-        7170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  humanized photoactivated adenylyl cyclase with cMyc Tag driven by 
            neuron-specific promoter, plus red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7170)
  TITLE     Thomas Oertner Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV hSyn1 bPAC cMyc T2A tDimer
FEATURES             Location/Qualifiers
     source          1..7170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   422..551
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(613..1201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(693..712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1372..2163,2164..2232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1995..2014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2233..2337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2405..2423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2461..2483)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2583..2602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2619..3074)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2756..2777)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2968..2987
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3149..3289
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3160..3289
                     /label=AAV2 ITR
     polyA_signal    3329..3805
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     3587..3606
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     3872..3888
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3873..3889
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3892..4480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3998..4009
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4407..4427
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4508..5182)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     CDS             complement(5225..5902)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     regulatory      5895..5904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5899..5908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5924..5977)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5990..6019)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(join(7096..7170,1..373))
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gcgcagatgg tcgcgcccgt gcccccctat ctcgcgcctc gcgtggtgcg gtccggctgg
       61 gccggcggcg gcgcggacgc gaccaaggtg gccgggaagg ggagtttgcg ggggaccggc
      121 gagtgacgtc agcgcgcctt cagtgctgag gcggcggtgg cgcgcgccgc caggcggggg
      181 cgaaggcact gtccgcggtg ctgaagctgg cagtgcgcac gcgcctcgcc gcatcctgtt
      241 tcccctcccc ctctctgata ggggatgcgc aatttgggga atgggggttg ggtgcttgtc
      301 cagtgggtcg gggtcggtcg tcaggtaggc acccccaccc cgcctcatcc tggtcctaaa
      361 acccacttgc actcatacgc agggccctct gcagctcgag tctagcgacg cgtgcggccg
      421 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
      481 gccgggcgac caaaggtcgc ccgacgcccg ggcggcctca gtgagcgagc gagcgcgcag
      541 ctgcctgcag gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2401 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     2461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     2521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     2581 tgcggcatca gagcagattg tactgagagt gcaccataaa attgtaaacg ttaatatttt
     2641 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat agaccgaaat
     2701 cggcaaaatc ccttataaat caaaagaata gcccgagata gagttgagtg ttgttccagt
     2761 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     2821 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag
     2881 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     2941 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctaaggc
     3001 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3061 gctacagggc gcgtactatg gttgctttga cgtatgcggt gtgaaatacc gcacagatgc
     3121 gtaaggagaa aataccgcat caggcgcccc tgcaggcagc tgcgcgctcg ctcgctcact
     3181 gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
     3241 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg cggccgctcg
     3301 gtccgcacgt ggttacctac aaaatcagaa ggacagggaa gggagcagtg gttcacgcct
     3361 gtaatcccag caatttggga ggccaaggtg ggtagatcac ctgagattag gagttggaga
     3421 ccagcctggc caatatggtg aaaccccgtc tctaccaaaa aaacaaaaat tagctgagcc
     3481 tggtcatgca tgcctggaat cccaacaact cgggaggctg aggcaggaga atcgcttgaa
     3541 cccaggaggc ggagattgca gtgagccaag attgtgccac tgcactccag cttggttccc
     3601 aatagacccc gcaggcccta caggttgtct tcccaacttg ccccttgctc cataccaccc
     3661 ccctccaccc cataatatta tagaaggaca cctagtcaga caaaatgatg caacttaatt
     3721 ttattaggac aaggctggtg ggcactggag tggcaacttc cagggccagg agaggcactg
     3781 gggaggggtc acagggatgc cacccgtaga tcatctctag actcgaggtc gaggccagag
     3841 aggccatcga atggcgcgcc gcgatcgcgt ctcgaggtcg acggtatcga tgcggggagg
     3901 cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca
     3961 gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc
     4021 agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg
     4081 atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc
     4141 agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag
     4201 tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa
     4261 acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca
     4321 gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat
     4381 acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac
     4441 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttttgctcga
     4501 agtcgaccta caggaacagg tggtggcggc cctcggagcg ctcgtactgt tccacgatgg
     4561 tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag tagccgggca
     4621 gctgcacggg cttcttggcc atgtagatgg tcttgaactc caccaggtag tggccgccgt
     4681 ccttcagctt cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc
     4741 gctcggtgga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggggggga
     4801 agttggtgcc gcggaacttc accttgtaga tcagcgtgcc gtcctgcagg gaggagtcct
     4861 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     4921 cggggaagga cagcttcttg tagtcgggga tgtcggcggg gtgcttcacg tacgccttgg
     4981 agccgtactg gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg
     5041 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     5101 tctcgaactc gtggccgttc acggagccct ccatgcgcac cttgaagcgc atgaactctt
     5161 tgatgacgtc ctcggaggag gcggtgccgg agctgccgct gccggtgctg ccggtgccat
     5221 gccccaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt
     5281 cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagctgca
     5341 cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca
     5401 gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg
     5461 tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg
     5521 tgccgcggaa cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca
     5581 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     5641 aggacagctt cttgtagtcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt
     5701 actggaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
     5761 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     5821 actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga
     5881 cgtcctcgga ggaggccacc atggcggccg cggatcccgg tgcagggccg ggattctcct
     5941 ccacgtcacc gcatgttaga agacttcctc tgccctcggc cgcgggtacc aggtcctcct
     6001 ccgagatcag cttttgctcg tacgtccgcg gcttgtcgtt ttccagggtc tgccggatca
     6061 gctgggtgat ctgcagggtg tcgtagtact tctgggcctc attcacggtg tagacctcga
     6121 tggcttcctg cttgcccttg acctggtggg ctcccaggtt gatgaaggtc cactgggcct
     6181 ggcagcactt cttcacgccg gctgtaaagg ccagggcgta gggcagctgt cttgtcaggg
     6241 cttccagcct ggcggccacg ttcacggcgt cccccagcag ggtgtggtcc atcttcaggc
     6301 tgctgcccat gttgccctcg atcacgtggc cgtagctcag gccgatgccg gtgtacagca
     6361 ggtgcagggg gttggtggcc tccacgtggt gccgcagctg cttcagctcg gagatgatgt
     6421 ccaggctggt ccggatggcg gcgtcgccct gctctttggt gaagctggcc atcacgcagt
     6481 cgccgatgaa cttggtcact tcgccgccgt aggcgctgat gatccgggtg cagatgctga
     6541 agtaccggtt gaccaggatg accacctcgt tcacgggcag cttctcggtc agggtgctga
     6601 aggccaggat gtcgctgaag aagatgatct tctcgaccag ctggggctcc acggtcaggg
     6661 ggttgatgcc ctggttgatc aggtagatca ctctggcggg catgtacttt tccagcaccc
     6721 ggtggctctg ggtgatggtc tgcagcaggc tcttgatggg ctggatcatc agctcgctgt
     6781 tctcgttcag gttgatggtt ttcatggccc agttggggaa catccggtcg gtgatgtcgt
     6841 actcggtttt caggcacagg atgttggtgt gccggtcgtc caccaggatt ttcttgtaca
     6901 gcttgtccac cttctcgttc tcgccttcca ggatctggaa gaacaggccc tgcaggtaca
     6961 gcagcacgcc ggtgatgttg tcccgctggt tgttcttgat ggacaccttg ccgatccgct
     7021 ggatctcttc caggctcagg tggccgctga tcttgctgat gtacaccagc cgcttcatca
     7081 tggtggcgaa ttcgactgcg ctctcaggca cgacacgact cctccgctgc ccaccgcaga
     7141 ctgaggcagc gctgagtcgc cggcgccgca
//
