LOCUS       sequence-201344-       11698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Pfeiffer BD, Jenett A, Hammonds AS, Ngo TT, Misra S, Murphy C, 
            Scully A, Carlson JW, Wan KH, Laverty TR, Mungall C, Svirskas R, 
            Kadonaga JT, Doe CQ, Eisen MB, Celniker SE, Rubin GM.
  TITLE     Tools for Neuroanatomy and Neurogenetics in Drosophila
  JOURNAL   Tools for neuroanatomy and neurogenetics in Drosophila
  PUBMED    18621688
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tools for 
            neuroanatomy and neurogenetics in Drosophila"
COMMENT     Sequence Label: pBPGUw
FEATURES             Location/Qualifiers
     source          1..11698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..2646
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(3142..3158)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3142..3158)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4169..4783,4854..4985,5189..5504,5566..6220,
                     6295..6568,6970..7041))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7493..7511
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7736..7805
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7892..8480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7972..7991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8651..9442,9443..9511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9274..9293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9512..9616)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9684..9702
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    9730..9854
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9879..9884,9885..9902,9903..9909)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9963..10622
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10029..10048)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10964..11269
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11148..11167
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11310..11434)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
       61 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
      121 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
      181 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt
      241 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
      301 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
      361 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
      421 caaagacagt tgactgtatc gattgactcg gcagctcatc atgataactc cacaattccg
      481 ttggatttta tgcccaggga tgctcttcat ggatttgatt ggtctgaaga ggatgacatg
      541 tcggatggct tgcccttcct gaaaacggac cccaacaata atgggttctt tggcgacggt
      601 tctctcttat gtattcttcg atctattggc tttaaaccgg aaaattacac gaactctaac
      661 gttaacaggc tcccgaccat gattacggat agatacacgt tggcttctag atccacaaca
      721 tcccgtttac ttcaaagtta tctcaataat tttcacccct actgccctat cgtgcactca
      781 ccgacgctaa tgatgttgta taataaccag attgaaatcg cgtcgaagga tcaatggcaa
      841 atccttttta actgcatatt agccattgga gcctggtgta tagaggggga atctactgat
      901 atagatgttt tttactatca aaatgctaaa tctcatttga cgagcaaggt cttcgagtca
      961 ggttccataa ttttggtgac agccctacat cttctgtcgc gatatacaca gtggaggcag
     1021 aaaacaaata ctagctataa ttttcacagc ttttccataa gaatggccat atcattgggc
     1081 ttgaataggg acctcccctc gtccttcagt gatagcagca ttctggaaca aagacgccga
     1141 atttggtggt ctgtctactc ttgggagatc caattgtccc tgctttatgg tcgatccatc
     1201 cagctttctc agaatacaat ctccttccct tcttctgtcg acgatgtgca gcgtaccaca
     1261 acaggtccca ccatatatca tggcatcatt gaaacagcaa ggctcttaca agttttcaca
     1321 aaaatctatg aactagacaa aacagtaact gcagaaaaaa gtcctatatg tgcaaaaaaa
     1381 tgcttgatga tttgtaatga gattgaggag gtttcgagac aggcaccaaa gtttttacaa
     1441 atggatattt ccaccaccgc tctaaccaat ttgttgaagg aacacccttg gctatccttt
     1501 acaagattcg aactgaagtg gaaacagttg tctcttatca tttatgtatt aagagatttt
     1561 ttcactaatt ttacccagaa aaagtcacaa ctagaacagg atcaaaatga tcatcaaagt
     1621 tatgaagtta aacgatgctc catcatgtta agcgatgcag cacaaagaac tgttatgtct
     1681 gtaagtagct atatggacaa tcataatgtc accccatatt ttgcctggaa ttgttcttat
     1741 tacttgttca atgcagtcct agtacccata aagactctac tctcaaactc aaaatcgaat
     1801 gctgagaata acgagaccgc acaattatta caacaaatta acactgttct gatgctatta
     1861 aaaaaactgg ccacttttaa aatccagact tgtgaaaaat acattcaagt actggaagag
     1921 gtatgtgcgc cgtttctgtt atcacagtgt gcaatcccat taccgcatat cagttataac
     1981 aatagtaatg gtagcgccat taaaaatatt gtcggttctg caactatcgc ccaataccct
     2041 actcttccgg aggaaaatgt caacaatatc agtgttaaat atgtttctcc tggctcagta
     2101 gggccttcac ctgtgccatt gaaatcagga gcaagtttca gtgatctagt caagctgtta
     2161 tctaaccgtc caccctctcg taactctcca gtgacaatac caagaagcac accttcgcat
     2221 cgctcagtca cgccttttct agggcaacag caacagctgc aatcattagt gccactgacc
     2281 ccgtctgctt tgtttggtgg cgccaatttt aatcaaagtg ggaatattgc tgatagctca
     2341 ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac tcaaacaaat
     2401 tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt catgaataat
     2461 gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc acctggttgg
     2521 acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa taccactaca
     2581 atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa cccaaaaaaa
     2641 gagtaaaatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg
     2701 caccatctca atttctttca tttatacatc gttttgcctt cttttatgta actatactcc
     2761 tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa atgactagaa
     2821 ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac gagggcttat
     2881 tcagaagctt atcgataccg tcgactaaag ccaaatagaa aattattcag ttcctggctt
     2941 aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat gtaaataact
     3001 aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat attagctact
     3061 tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt cagggagtga
     3121 gtttgcttaa aaactcgttt agatccacta gttctagagc ggccgctggc cacgggtgcg
     3181 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     3241 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     3301 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     3361 gaagtcagcg ccctgcacca ttatgttccg gaggcgcgcc ctagttccag tgaaatccaa
     3421 gcattttcta aattaaatgt attcttatta ttatagttgt tatttttgat atatataaac
     3481 aacactatta tgcccaccat ttttttgaga tgcatctaca caaggaacaa acactggatg
     3541 tcactttcag ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa aattacctta
     3601 aaaagtcata atattaaatt agaataaata tagctgtgag ggaaatatat acaaatatat
     3661 tggagcaaat aaattgtaca tacaaatatt tattactaat ttctattgag acgaaatgaa
     3721 ccactcggaa ccatttgagc gaaccgaatc gcgcggaact aacgacagtc gctccaaggt
     3781 cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta
     3841 cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat
     3901 ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc
     3961 aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa
     4021 ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca
     4081 ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa
     4141 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     4201 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagacccacg tagtccagcg
     4261 gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg
     4321 tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca
     4381 gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg
     4441 gcaccgagcc cgagttcaag aagaagccgc caaagagcag gaatggtatg ataaccggcg
     4501 gacccacaga cagcgccatc gaggtcgagg agctggcgca ggatattaga tatccgaagg
     4561 acgttgacac attggccacc agagtgacca gcgccaggca gttgaagaag tgcagcactc
     4621 cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa
     4681 aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt gtcacagcga taaagtcgac
     4741 ttcgggcctc cctcataaaa actggcagct ctgaggtgaa cacctaaatc gaatcgattc
     4801 attagaaagt tagtaaatta ttgaaatgca aatgtattct aaacatgact tacatttatc
     4861 gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga ggaagatggc tccgttgata
     4921 ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga tgaggccaat caagatggca
     4981 accatctgca aattaaaatg ttactcgcat ctcattaata ttcgcgagtt aaatgaaatt
     5041 tatttatctt ctgcaaaact ataaactata catctcattg aaaaaaacta agaagggtgt
     5101 ggaatcaggc aattctatct aaaatctagc gaatttgttt ccaagaattg taagcgttat
     5161 atcatttgtt tccactggaa ccactcaccg ttgtctgaat aagtcgcact tttacgagga
     5221 gtggttcctt gagcaccgac agccaggatc gccacaggac cgcccggaac tgcatgaacc
     5281 aggtggcctt gtaggtgtac ccattctccg gctgctccag tggcttctcc agatttttgg
     5341 tggccaacaa ctgctccata tcccgggcta ctttgctaat ggcaaaattg tcgcatatct
     5401 tggcgatccg atcacgggac tcgatctccc gtccgggcac aacggccaac acctgtacgt
     5461 aaaagtccgc cggattgtag ttggtaggac actgggcacc cacgctggat aggagttgag
     5521 atgttatgta atactagata cccttaataa acacatcgaa ctcactagga aaagaagtcg
     5581 acggcttcgc tgggagtgcc caagaaagct accctgccct cggccatcag aaggatcttg
     5641 tcaaagagct caaacagctc ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc
     5701 ttctgcgaca gcttcttcag cacctggacg acgctgtggg cggtaaagga gtccagtccg
     5761 gaggtgggct catcgcagat cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc
     5821 agacgcttcc tttctccgcc ggacagacct ttcaccctgc cgggcacacc gatgatcgtg
     5881 tgctgacatt tgctgagcga aagctcctgg atcacctgat ccacgcgggc cactcgctgc
     5941 cgataggtca gatgtcgtgg catccgcacc atggcttgga aaatcaggtg ttccctggcc
     6001 gttagggagc cgataaagag gtcatcctgc tggacatagg cgcacctggc ctgcatctcc
     6061 ttggcgtcca caggttggcc attgagcagt cgcatcccgg atggcgatac ttggatgccc
     6121 tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct ttccggcacc ggaactgccc
     6181 atcacggcca aaagttcgcc cggataggcc acgccgcaaa ctgagtttca aattggtaat
     6241 tggacccttt attaagattt cacacagatc agccgactgc gaatagaaac tcaccgttct
     6301 tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc
     6361 ggttgaccag ctgccgccat ccggagcccg gctgattgac cgccccaaag atgtccatat
     6421 tgtgccaggc ataggtgagg ttctcggcta gttggccgct ccctgaaccg gagtcctccg
     6481 gcggactggg tggccggagc gtgccgtagt ttttggcctg cccgaagccc tggttaatgc
     6541 agctctgcga agccgctccg ctgtcaccct gcaatgatag gggatctcaa atatcaacta
     6601 caagcgttat gctcatctaa ccccgaacaa aaagtacccc gaagtatcct acgaagtagg
     6661 tttatacttt tatttatttt ttgtgcatct aggatcagct taaaatatct ggttgttata
     6721 ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt agatgtcttg atcatgatat
     6781 gatctcaaaa attgtcttat atagcgagaa cagctaccag aataatctgt ttcgtgtcac
     6841 tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc gcagttctca cgccgcagac
     6901 aatttgatgt tgcaatcgca gttcctatag atcaagtgaa cttaagatgt atgcacatgt
     6961 actactcaca ttgttcagat gctcggcaga tgggtgtttg ctgcctccgc gaattaatag
     7021 ctcctgatcc tcttggccca ttgccgggat ttttcacact ttcccctgct tacccaccca
     7081 aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg agaggagttt
     7141 tggcacagca ctttgtgttt aattgatggc gtaaaccgct tggagcttcg tcacgaaacc
     7201 gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt aacgctacaa acggtggcga
     7261 aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca gagaaggagg
     7321 caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg aaagaacctg
     7381 gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag ttcaatgata
     7441 tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa atgggtttta
     7501 ttaacttaca tacatactag gcgcgcccat atgttcggct tgtcgacatg cccgccgtga
     7561 ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc
     7621 ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc
     7681 gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg gaccagatgg
     7741 gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg caccgcggct
     7801 tcgagaccgt gacctacatc gtcgacggta acatgtgagc aaaaggccag caaaaggcca
     7861 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7921 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7981 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8041 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8101 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8161 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8221 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8281 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8341 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8401 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8461 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8521 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8581 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8641 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8701 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8761 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8821 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8881 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8941 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9001 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9061 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9121 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9181 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9241 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9301 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9361 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9421 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9481 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9541 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9601 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9661 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
     9721 tcgtttatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     9781 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     9841 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     9901 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     9961 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
    10021 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
    10081 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
    10141 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
    10201 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
    10261 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
    10321 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
    10381 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
    10441 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
    10501 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
    10561 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
    10621 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
    10681 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
    10741 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
    10801 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
    10861 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
    10921 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
    10981 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
    11041 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
    11101 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
    11161 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
    11221 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
    11281 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
    11341 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
    11401 acgtttctcg ttcagctttc ttgtacaaag tggtgataaa cggccggccg agctcgcccg
    11461 gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag ttgtgaatga
    11521 atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat aagccaactt
    11581 tgaatcacaa gacgcatacc aaacggtacc agatccggga tcccgtaagc aaataaacaa
    11641 gcgcagctga acaagctaaa caatctgcag cccaagcttg aagcaagcct cctgaaag
//
