LOCUS       sequence-201380-       14581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr II, 
            corresponding to RDE-1 expressed specifically in the intestine, for 
            tissue-specific RNAi..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14581)
  TITLE     Ewbank lab plasmids
REFERENCE   2  (bases 1 to 14581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pNP160
FEATURES             Location/Qualifiers
     source          1..14581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12442..12461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12561..12583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12621..12639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12707..12811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12812..12880,12881..13672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13030..13049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13843..14431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14332..14351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgcaaca
     6901 aacgtgggat gtaaccatgg aagtgagaat aattgatgga atagctaatg agctgaagct
     6961 gagtagatca gagactactt tattctaaaa agtacagtag ttggaaaatt acatgtttca
     7021 tttctaattt tcaaggaaaa gctagtaatt accgtaatct tgtttgtacc tgaaatttta
     7081 tgtactgcag gtgaccaagt atgtttgagg catgacttca cacacctaac tgataaaggc
     7141 ctctatcaca aactagagtt gtgacggaaa attcaacttc tcagaatata gctcaaaatc
     7201 tatcaaattt tattttcaaa aatccaaata attgtgcacg caatgtactt actgcttcat
     7261 aaagttcaga agaattggat aaatttgaat gaagttttca aagcttttat cagtgactgt
     7321 acattgtgat aggcttgtgc tgttatcagc tgcctcaaat aggttgtcgc ttgaaaattt
     7381 atataaaagg cctaccagca gacatgagaa tcaagcttca aaggctctac tagtgatgaa
     7441 acatgtcctc gaattttccc gaattggaaa aaggatttta tcgtcattct ctcgatccgg
     7501 tatgatcaat tattagcagc tataagatat ataagtttga tattaatatt ataggagatg
     7561 aaatggcttg cgaggcccac tggtaaatgc gacggcaaat tctatgagaa gaaagtactt
     7621 cttttggtaa attggttcaa gttctccagc aaaatttacg atcgggaata ctacgagtat
     7681 gaagtgaaaa tgacaaagga agtattgaat agaaaaccag gaaaaccttt cccaaaaaag
     7741 acagaaattc caatgtaagt gcttgtaaat tagtcaaaac taattttatt tttcagtccc
     7801 gatcgtgcaa aactcttctg gcaacatctt cggcatgaga agaagcagac agattttatt
     7861 ctcgaagact atgtttttga tgaaaaggac actgtttata gtgtttgtcg actgaacact
     7921 gtcacatcaa aaatgctggt ttcggagaaa gtagtaaaaa aggattcgga gaaaaaagat
     7981 gaaaaggatt tggagaaaaa aatcttatac acaatgatac ttacctatcg taaaaaattt
     8041 cacctgaact ttagtcgaga aaatccggaa aaagacgaag aagcgaatcg gagttacaaa
     8101 ttcctgaagg tttatgaaaa acacgcatta taacaaacaa aattagcttt cagaatgtta
     8161 tgacccagaa agttcgctac gcgccttttg tgaacgagga gattaaagtg tgagttgcaa
     8221 taataataat aataatcacc tcaactcatt tatatatttt aagacaattc gcgaaaaatt
     8281 ttgtgtacga taataattca attctgcgag ttcctgaatc gtttcacgat ccaaacagat
     8341 tcgaacaatc attagaagta gcaccaagaa tcgaagcatg gtttggaatt tacattggaa
     8401 tcaaagaatt gttcgatggt gaacctgtgc tcaattttgc aagtaagttt gagaaactgc
     8461 gataaaaaat catgtgattt ttgttgaagt tgtcgataaa ctattctaca atgcaccgaa
     8521 aatgtctctt ctggattatc ttctcctaat tgtcgacccc cagtcgtgta acgatgatgt
     8581 acgaaaagat cttaaaacaa aactgatggc gggaaaaatg acaatcagac aagccgcgcg
     8641 gccaagaatt cgacaattat tggaaaattt gaagctgaaa tgcgcagaag tttgggataa
     8701 cgaaatgtta gtttaaatta ttcaaacaat taatatacaa attgattttc aggtcgagat
     8761 tgacagaacg acatctgaca tttctagatt tgtgcgagga aaactctctt gtttataaag
     8821 tcactggtaa atcggacaga ggaagaaatg caaaaaagta cgatactaca ttgttcaaaa
     8881 tctatgagga aaacaaaaag ttcattgagt ttccccacct accactagtc aaagttaaaa
     8941 gtggagcaaa agaatacgct gtaccaatgg aacatcttga agttcatgag aagccacaaa
     9001 gatacaagaa tcgaattgat ctggtgatgc aagacaagtt tctaaagcga gctacacgaa
     9061 aacctcacga ctacaaagaa aataccctaa aaatgctgaa agaattggat ttctcttctg
     9121 aagagctaaa ttttgttgaa agatttggat tatgctccaa acttcagatg atcgaatgtc
     9181 caggaaaggt tttgaaagag ccaatgcttg tgaatagtgt aaatgaacaa attaaaatga
     9241 caccagtgat tcgtggattt caagaaaaac aattgaatgt ggttcccgaa aaagaacttt
     9301 gctgtgctgt ttttgtagtc aacgaaacag cgggaaatcc atgcttagaa gagaacgacg
     9361 ttgtgtaagt gttttctacg tagattattc cgaaatattt tcagtaagtt ctacaccgaa
     9421 ctaattggtg gttgcaagtt ccgtggaata cgaattggtg ccaatgaaaa cagaggagcg
     9481 caatctatta tgtacgacgc gacgaaaaat gaatatgccg taagtttcag aaaattgaaa
     9541 gtttttaaat atcatattta cagttctaca aaaattgtac actaaatacc ggaatcggta
     9601 gatttgaaat agccgcaaca gaagcgaaga atatgtttga acgtcttccc gataaagaac
     9661 aaaaagtctt aatgttcatt atcatttcca aacgacaact gaatgcttac ggttttgtga
     9721 aacattattg cgatcacacc atcggtgtag ctaatcagca tattacttct gaaacagtca
     9781 caaaagcttt ggcatcacta aggcacgaga aaggatcaaa acgaattttc tatcaaattg
     9841 cattgaaaat caacgcgaaa ttaggaggta ttaaccagga gcttgactgg tcagaaattg
     9901 cagaaatatc accagaagaa aaagaaagac ggaaaacaat gccattaact atgtatgttg
     9961 gaattgatgt aactcatcca acctcctaca gtggaattga ttattctata gcggctgtag
    10021 tagcgagtat caatccaggt ggaactatct atcgaaatat gattgtgact caagaagaat
    10081 gtcgtcccgg tgagcgtgca gtggctcatg gacgggaaag aacagatatt ttggaagcaa
    10141 agttcgtgaa attgctcaga gaattcgcag aagtgagttg tcttgagtat ttaaaagatc
    10201 tctgggattt ttaatttttt tgtaaacttt cagaacaacg acaatcgagc accagcgcat
    10261 attgtagtct atcgagacgg agttagcgat tcggagatgc tacgtgttag tcatgatgag
    10321 cttcgatctt taaaaagcga agtaaaacaa ttcatgtcgg aacgggatgg agaagatcca
    10381 gagccgaagt acacgttcat tgtgattcag aaaagacaca atacacgatt gcttcgaaga
    10441 atggaaaaag ataagccagt ggtcaataaa gatcttactc ctgctgaaac agatgtcgct
    10501 gttgctgctg ttaaacaatg ggaggaggat atgaaagaaa gcaaagaaac tggaattgtg
    10561 aacccatcat ccggaacaac tgtggataaa cttatcgttt cgaaatacaa attcgatttt
    10621 ttcttggcat ctcatcatgg tgtccttggt acatctcgtc caggacatta cactgttatg
    10681 tatgacgata aaggaatgag ccaagatgaa gtctatgtaa gcgttttgaa tagcagttag
    10741 cgattttagg attttgtaat ccgcatatag ttattataaa aaaatgtttc agaaaatgac
    10801 ctacggactt gcttttctct ctgctagatg tcgaaaaccc atctcgttgc ctgttccggt
    10861 tcattatgct catttatcat gtgaaaaagc gaaagagctt tatcgaactt acaaggaaca
    10921 ttacatcggt gactatgcac agccacggac tcgacacgaa atggaacatt ttctccaaac
    10981 taacgtgaag taccctggaa tgtcgttcgc ataacatttt gcaaaagtgt cgcccgtttc
    11041 aatcaaattt ttcaattgta gatattgtac ttactttttt ttaaagcccg gtttcaaaaa
    11101 ttcattccat gactaacgtt ttcataaatt acttgaaatt tattctgtgt ttattattta
    11161 ttacctctaa atttcgtttt gaacgtgagc atcatatctt aaactactta ttgataacgg
    11221 tttcataaag ataggtaatt tgatttttaa aatgttttct ctctgcaggt gatttcacga
    11281 ctatgcgcga tgccgtatca caactgagaa aaaagacaaa acttattgaa cagtgttcct
    11341 ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac gcaatcaaaa
    11401 acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta ccattattgt
    11461 ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt cttgcattta
    11521 ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag tcacctctaa
    11581 ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac cgtaattaga
    11641 aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa tatggcattt
    11701 tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac aagccaaatt
    11761 caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa caatggcgaa
    11821 gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa gacttcttga
    11881 aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta agggctatag
    11941 atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg tgcgaaggaa
    12001 tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt tattttttca
    12061 aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag atcatatcca
    12121 tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa ggaaaatctc
    12181 aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcca cgtgacgtcg
    12241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
    12301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
    12361 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
    12421 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
    12481 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    12541 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    12601 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    12661 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    12721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    12781 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    12841 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    12901 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    12961 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    13021 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    13081 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    13141 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    13201 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    13261 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    13321 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    13381 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    13441 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    13501 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    13561 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    13621 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    13681 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    13741 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    13801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    13861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    13921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    13981 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    14041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    14101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    14161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    14221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    14281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    14341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    14401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    14461 cctggtcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    14521 ggataaccgt attaccgcct ttgagtgagc tgataccagc ggataacaat ttcacagggc
    14581 c
//
