LOCUS       sequence-201554-       10201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  b2AR-SPARK protease component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY
  TITLE     Time-gated detection of protein-protein interactions with 
            transcriptional readout.
  JOURNAL   Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233.
  PUBMED    29189201
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-30"; volume: "6. doi"; pages: " 10.7554/eLife.30233"
COMMENT     Sequence Label: pLX208-beta-arrestin2-HA-TEV219
FEATURES             Location/Qualifiers
     source          1..10201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1828..1854
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1855..1881
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2587..3175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2640..2660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3058..3069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3247..3480
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3558..3679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3595..3614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3649..3668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3719..3854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3874..3893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3875..3893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3903..3920)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3903..3919)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3912..3934)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4061..4516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4148..4167)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4358..4379
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4542..4646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4647..4715,4716..5507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4865..4884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5678..6266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6167..6186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6420..6437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6554..6575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6590..6595,6596..6613,6614..6620)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6628..6644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6633..6655
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6652..6668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6652..6668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6687..6707
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6689..6707
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6735..6961
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6962..7142
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7189..7314
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7807..8040
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8225..8269
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8444..8944
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             8956..9975
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    10032..10149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atccaccatg
      601 ggtgaaaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg
      661 tacttgggca agcgtgactt tgtggatcac ttggacaaag tggatcctgt cgatggtgtg
      721 gtgcttgtgg atcctgacta cttgaaggac cggaaagtgt ttgtgaccct cacctgtgcc
      781 ttccgctatg gccgagaaga cctggatgta ctgggcctgt ctttccgcaa agatctgttc
      841 atcgccacct accaggcctt cccccccatg cccaacccac ctcggccccc cacccgccta
      901 caggaccgac tgctgaagaa gttgggccag cacgcccacc cctttttttt cacaataccc
      961 cagaatttgc cttgctccgt cacactgcag ccaggaccgg aggacacagg gaaggcctgt
     1021 ggagtagact ttgagattcg agccttctgt gccaaatcta tagaagaaaa aagccacaaa
     1081 aggaactccg tgcggcttat catcagaaag gtacagtttg ctcctgagac acccggcccc
     1141 cagccatcag ctgaaaccac acgccacttc ctcatgtctg accggaggtc cctgcaccta
     1201 gaggcttccc tggacaaaga gctgtactac catggggaac ccctcaatgt caacgtccac
     1261 gtcaccaaca attctgccaa aaccgtcaag aagatcagag tgtctgtgag acagtatgcc
     1321 gacatttgcc tcttcagcac cgcgcagtac aagtgtcctg tggctcagct tgaacaagat
     1381 gaccaggtgt ctcccagttc cacattctgc aaggtgtaca ccataacccc gctgctcagt
     1441 gacaaccgag agaagcgtgg ccttgccctt gatgggcaac tcaagcacga agacaccaac
     1501 ctggcttcca gcaccattgt gaaggaggga gccaacaagg aggtgctggg aatcctagta
     1561 tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc cgtggagcta
     1621 cctttcgtcc taatgcaccc caagccccac gaccacatca cccttccccg accccagtca
     1681 gccccccggg aaatagacat ccctgtggat accaacctca ttgaattcga taccaactat
     1741 gccacagacg acgacatcgt gtttgaggac tttgcgaggc ttcggctgaa ggggatgaag
     1801 gatgacgact gtgatgacca gttctgctac ccatacgatg tgccagatta cgcctatccc
     1861 tatgacgtcc ctgattacgc tggtggaagt ggatcaggca gcggtggatc tggagaaagc
     1921 ttgtttaagg gaccacgtga ttacaacccg atatcgagca ccatttgtca tttgacgaat
     1981 gaatctgatg ggcacacaac atcgttgtat ggtattggat ttggtccctt catcattaca
     2041 aacaagcact tgtttagaag aaataatgga acactgttgg tccaatcact acatggtgta
     2101 ttcaaggtca agaacaccac gactttgcaa caacacctca ttgatgggag ggacatgata
     2161 attattcgca tgcctaagga tttcccacca tttcctcaaa agctgaaatt tagagagcca
     2221 caaagggaag agcgcatatg tcttgtgaca accaacttcc aaactaagag catgtctagc
     2281 atggtgtcag acactagttg cacattccct tcatctgatg gcatattctg gaagcattgg
     2341 attcaaacca aggatgggca gtgtggcagt ccattagtat caactagaga tgggttcatt
     2401 gttggtatac actcagcatc gaatttcacc aacacaaaca attatttcac aagcgtgccg
     2461 aaaaacttca tggaattgtt gacaaatcag gaggcgcagc agtgggttag tggttggcga
     2521 ttaaatgctg actcagtatt gtggggtggc cataaagttt tcatggtgta aacgcgttaa
     2581 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2641 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2701 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2761 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2821 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2881 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2941 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3001 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3061 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3121 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3181 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3241 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3301 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3361 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3421 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3481 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3541 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3601 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3661 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3721 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3841 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3901 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3961 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4021 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4081 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4141 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4201 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4261 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4321 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4381 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4441 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4501 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4621 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4681 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4741 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4801 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4861 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4921 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4981 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5041 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5101 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5161 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5221 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5281 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5341 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5401 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5461 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5521 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5821 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6241 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6301 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6361 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6421 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6481 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6541 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6601 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6661 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6721 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6781 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6841 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6901 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6961 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7141 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7201 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7261 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7321 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7381 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7441 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7501 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7561 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7621 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7681 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7741 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7801 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7861 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7921 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7981 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8041 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8101 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8161 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8221 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8281 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8341 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8401 accattatcg tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg
     8461 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     8521 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     8581 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     8641 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     8701 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     8761 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     8821 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     8881 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     8941 ccagggggta ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg
     9001 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct ccgagggcga agaatctcgg
     9061 gctttcagct tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat
     9121 ggtttctaca aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc
     9181 gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca
     9241 cagggtgtca ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc
     9301 gccgaggcca tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca
     9361 ttcggacctc aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct
     9421 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc
     9481 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     9541 cacgccgatt tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt
     9601 gactggagcg aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     9661 aggccctggt tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag
     9721 cttgcaggat ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat
     9781 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca
     9841 atcgtccggt ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc
     9901 gtctggaccg atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact
     9961 cgtcctaggg caaaggatct gcagtaatga gaattctaga tcttgagaca aatggcagta
    10021 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10081 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10141 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg
    10201 g
//
