LOCUS       sequence-201567-        5676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that 
            targets the CACTA1 promoter in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF2CACTA1_3xFLAG_TET1cd
FEATURES             Location/Qualifiers
     source          1..5676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2518..2589
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2629..2653
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4856..4880)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtctagctc aacagagctt ttaacccaaa ttggtacaat agaatacaac tttagatcat
       61 aattctcaaa agaaagagat tccttagcta ttctatctgc cactccattt ccttctcggc
      121 ttgtatgcac aagcataaaa tcctcaaact tgctaagtag atactttatg tcttggataa
      181 ttggattgag acttgacaag cataactttc atgtaaccaa agacacaagt tgctgagaat
      241 ccacctcaaa aatgatcttc ctataattga atcgggataa tgacagcaca gcccatctaa
      301 gagcctccac ttctacttcc agcacgcttc ttacttttac cacagctctt gcacctaacc
      361 ataacacctt ccctgtatga tcgcgaagca cccaccctaa gccacatttt aatccttctg
      421 ttggccatgc cccatcaaag ttgcacttaa cccaagattg tggtggagct tcccatgttt
      481 ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta cattctgagc ctctttcctt
      541 ctaatccact catctgcatc ttcttgtgtc cttactaata cctcattggt tccaaattcc
      601 ctccctttaa gcaccagctc gtttctgttc ttccacagcc tcccaagtat ccaagggact
      661 aaagcctcca cattcttcag atcaggatat tcttgtttaa gatgttgaac tctatggagg
      721 tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg aacttattca
      781 aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca
      841 ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg
      901 aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt
      961 tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc
     1021 aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag
     1081 ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa
     1141 aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc
     1201 aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa
     1261 aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg
     1321 atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa
     1381 gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct
     1441 caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca
     1501 cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc
     1561 ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa
     1621 ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca
     1681 aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc
     1741 aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg
     1801 ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt
     1861 tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga
     1921 tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat
     1981 taacagatgg ttgagaaacc ttacaagtgc ccggagtgcg gaaagtcttt ttcacaaaac
     2041 tcaaccttga cggagcatca gcgaacacat accggggaaa agccgtataa gtgtccggaa
     2101 tgcgggaaat ctttttctag gtctgataac ttggtgagac accagcgtac acacacaggt
     2161 gaaaagcctt acaagtgccc agaatgcgga aagagcttca gcaccaccgg caatctgacc
     2221 gtacatcagc gaactcatac aggtgagaaa ccctataagt gtcctgagtg cggaaaatct
     2281 ttcagccaat catcaaattt agtgagacat cagaggaccc acacagggga gaagccgtat
     2341 aaatgtcccg agtgtgggaa aagtttctct cgatccgata acctggtacg acatcagcga
     2401 acccatactg gtgagaagcc ctataaatgc cctgaatgcg gcaaatcctt ttccacgtcc
     2461 gggagcctag ttaggcatca acgaacccat accggtaacc gcgccaagct tggtaccgac
     2521 tacaaagacg atgacgacaa agactacaaa gacgatgacg acaaagacta caaagacgat
     2581 gacgacaaag gatcccttga ggttcttttc ggcgcgccaa gctatcaaac aagtttgtac
     2641 aaaaaagcag gctccgcggc cgcccccttc accatgggat ccctgcccac ctgcagctgt
     2701 cttgatcgag ttatacaaaa agacaaaggc ccatattata cacaccttgg ggcaggacca
     2761 agtgttgctg ctgtcaggga aatcatggag aataggtatg gtcaaaaagg aaacgcaata
     2821 aggatagaaa tagtagtgta caccggtaaa gaagggaaaa gctctcatgg gtgtccaatt
     2881 gctaagtggg ttttaagaag aagcagtgat gaagaaaaag ttctttgttt ggtccggcag
     2941 cgtacaggcc accactgtcc aactgctgtg atggtggtgc tcatcatggt gtgggatggc
     3001 atccctcttc caatggccga ccggctatac acagagctca cagagaatct aaagtcatac
     3061 aatgggcacc ctaccgacag aagatgcacc ctcaatgaaa atcgtacctg tacatgtcaa
     3121 ggaattgatc cagagacttg tggagcttca ttctcttttg gctgttcatg gagtatgtac
     3181 tttaatggct gtaagtttgg tagaagccca agccccagaa gatttagaat tgatccaagc
     3241 tctcccttac atgaaaaaaa ccttgaagat aacttacaga gtttggctac acgattagct
     3301 ccaatttata agcagtatgc tccagtagct taccaaaatc aggtggaata tgaaaatgtt
     3361 gcccgagaat gtcggcttgg cagcaaggaa ggtcgaccct tctctggggt cactgcttgc
     3421 ctggacttct gtgctcatcc ccacagggac attcacaaca tgaataatgg aagcactgtg
     3481 gtttgtacct taactcgaga agataaccgc tctttgggtg ttattcctca agatgagcag
     3541 ctccatgtgc tacctcttta taagctttca gacacagatg agtttggctc caaggaagga
     3601 atggaagcca agatcaaatc tggggccatc gaggtcctgg caccccgccg caaaaaaaga
     3661 acgtgtttca ctcagcctgt tccccgttct ggaaagaaga gggctgcgat gatgacagag
     3721 gttcttgcac ataagataag ggcagtggaa aagaaaccta ttccccgaat caagcggaag
     3781 aataactcaa caacaacaaa caacagtaag ccttcgtcac tgccaacctt agggagtaac
     3841 actgagaccg tgcaacctga agtaaaaagt gaaaccgaac cccattttat cttaaaaagt
     3901 tcagacaaca ctaaaactta ttcgctgatg ccatccgctc ctcacccagt gaaagaggca
     3961 tctccaggct tctcctggtc cccgaagact gcttcagcca caccagctcc actgaagaat
     4021 gacgcaacag cctcatgcgg gttttcagaa agaagcagca ctccccactg tacgatgcct
     4081 tcgggaagac tcagtggtgc caatgctgca gctgctgatg gccctggcat ttcacagctt
     4141 ggcgaagtgg ctcctctccc caccctgtct gctcctgtga tggagcccct cattaattct
     4201 gagccttcca ctggtgtgac tgagccgcta acgcctcatc agccaaacca ccagccctcc
     4261 ttcctcacct ctcctcaaga ccttgcctct tctccaatgg aagaagatga gcagcattct
     4321 gaagcagatg agcctccatc agacgaaccc ctatctgatg accccctgtc acctgctgag
     4381 gagaaattgc cccacattga tgagtattgg tcagacagtg agcacatctt tttggatgca
     4441 aatattggtg gggtggccat cgcacctgct cacggctcgg ttttgattga gtgtgcccgg
     4501 cgagagctgc acgctaccac tcctgttgag caccccaacc gtaatcatcc aacccgcctc
     4561 tcccttgtct tttaccagca caaaaaccta aataagcccc aacatggttt tgaactaaac
     4621 aagattaagt ttgaggctaa agaagctaag aataagaaaa tgaaggcctc agagcaaaaa
     4681 gaccaggcag ctaatgaagg tccagaacag tcctctgaag taaatgaatt gaaccaaatt
     4741 ccttctcata aagcattaac attaacccat gacaatgttg tcaccgtgtc cccttatgct
     4801 ctcacacacg ttgcggggcc ctataaccat tgggtctgaa agggtgggcg cgccgaccca
     4861 gctttcttgt acaaagtggt tcgataattc ttaattaact agttctagag cggccgccac
     4921 cgcggtggag ctcctaggtg agtctagaga gttaattaag acccgggact agtccctaga
     4981 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     5041 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     5101 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     5161 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     5221 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     5281 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     5341 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     5401 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     5461 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     5521 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     5581 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     5641 cgttgggcgg gtccagggcg aattttgcga cgagct
//
