LOCUS       sequence-201619-       12377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of SAP97alpha-EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 12377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FUap97GW (C4)
FEATURES             Location/Qualifiers
     source          1..12377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3843
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3680..3699
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6307..6316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6313..6315,6316..6318,6319..7032)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6313..6315,6316..6318,6319..7029)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6358..6379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6619..6638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6966..6987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7075..7663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7128..7148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7546..7557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8182..8362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8388..8405)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8394..8618
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8664..9092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8751..8770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8961..8982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9101..9121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9106..9435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9286..9421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9348..9367
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9483..9530
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9549..9923
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10053..10174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10090..10109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10144..10163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10223..10239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10223..10239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10236..10258)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10247..10263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10271..10277,10278..10295,10296..10301))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10316..10337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10454..10471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10625..11213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10705..10724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11384..12175,12176..12244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12007..12026
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12245..12349)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg tacgaattta acccgtgtcg gctccagatc tggcctccgc
     2641 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     2701 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     2761 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     2821 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     2881 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     2941 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     3001 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     3061 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     3121 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     3181 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     3241 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     3301 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     3361 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     3421 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     3481 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     3541 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     3601 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     3661 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     3721 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     3781 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     3841 gacgaagctt gggctgcagg tcgactctag accaccatgg attgcctgtg cattgtaact
     3901 acaaagaaat accggtatca ggacgaagag gtgctgcctt cagagcggat ttccccgcaa
     3961 gtcccaaatg aggtgctggg tccggagctg gttcacgtct cggagaagag cctgtcagag
     4021 attgagaatg tccacgggtt tgtctctcac tctcatatct cacccataaa ggcaaatcct
     4081 cctcctgtgc tggtcaacac agacagcttg gagacaccaa cttatgttaa tggtactgat
     4141 gcagattatg aatatgagga aatcacactt gaaaggggaa attcaggcct tggtttcagc
     4201 attgcaggag gtacagacaa cccacacatt ggagatgact caagtatttt catcaccaaa
     4261 attatcacgg gaggagcagc tgcccaggat ggaagattgc gggtaaatga ctgtatactg
     4321 agagtaaatg aagcagatgt tcgtgatgta acccacagca aagcagttga agcattgaaa
     4381 gaagctggat cgatagtgcg attatatgtg aaaaggcgga agccagcctc agaaaaaatc
     4441 atggaaataa aacttattaa aggccctaaa ggtcttgggt tcagcattgc tggaggtgtt
     4501 ggaaaccagc acattcctgg ggataacagc atctatgtaa ccaaaataat tgaaggaggt
     4561 gcagcacaca aggacggcaa acttcagatt ggagacaagc ttctagcagt gaacagtgtc
     4621 tgtttagaag aagttactca tgaagaagca gtgactgcct taaagaatac atctgatttt
     4681 gtttatttga aagtggcaaa accaacaagt atgtatataa atgatggcta tgcaccaccc
     4741 gacatcacta attcttcttc tcagtctgtt gacaaccatg ttagcccgtc ttcatacttg
     4801 ggccagactc cagcgtcacc agccagatac tcacccattt ctaaagcagt gcttggagat
     4861 gatgagatca ctagggaacc tagaaaagtt gttcttcatc gtggctcaac gggacttggt
     4921 ttcaacattg tgggaggcga agatggagaa gggattttta tctctttcat ccttgctggg
     4981 ggacctgctg acctaagcgg agagctcaga aaaggagatc gcattatatc ggtaaacagt
     5041 gttgacctca gagctgcaag tcatgaacaa gcagctgccg cattaaaaaa cgcaggccaa
     5101 gctgtcacta ttgtcgcaca gtatcggcct gaagagtaca gtcgttttga agctaaaatt
     5161 cacgacttgc gggagcagat gatgaacagc agtgtcagct cagggtcagg gtctctccga
     5221 accagccaaa agcggtccct ctatgtcaga gccctttttg attacgacaa gactaaagac
     5281 agtggccttc ccagtcaagg gttgaacttc aaattcggag atattctcca tgttatcaat
     5341 gcttctgatg acgagtggtg gcaggccaga caggtgaccc cagatggtga gagtgacgaa
     5401 gttggagtaa ttcctagtaa acgcagagtt gagaagaaag aacgagcccg attaaaaaca
     5461 gtcaaattca attctaaaac aagaggagat aaagggtcat tcaatgacaa gcgtaaaaag
     5521 aacctctttt cccgaaaatt tcccttctac aagaacaagg accagagtga gcaggaaaca
     5581 agtgatgctg accagcacgt aacttctaat gccagcgata gcgaaagtag ttaccgtggt
     5641 caagaagaat atgttttatc gtatgagccg gtgaatcagc aagaagttaa ttatacccga
     5701 ccagtcatca tattaggacc tatgaaagac agagtaaatg atgacttaat ctcagaattt
     5761 cctgacaaat ttggatcctg tgtccctcat acaactagac caaagcgtga ctatgaggtg
     5821 gatggacgag attaccattt tgtgacttca agagaacaga tggagaaaga tatccaggag
     5881 cataaattca ttgaagctgg ccagtataac aaccatctat acggaacgag tgtccaatct
     5941 gtgcgagcag tagcagagaa gggcaagcat tgtatccttg atgtgtctgg aaatgccatc
     6001 aagaggttgc agattgcaca gctctatcca atatctattt ttattaaacc caaatccatg
     6061 gaaaatatca tggaaatgaa taaacgccta acagacgaac aggccagaaa aacgtttgag
     6121 agagcagtga gactggagca ggagttcact gagcatttca cagctattgt ccagggagac
     6181 acgctggagg acatttacaa tcaagtgaag cagatcatag aagagcagtc tggtccttac
     6241 atctgggtcc cagcaaaaga aaaattagga tccggtggat caggaggttc aggtggaaga
     6301 tccatcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6361 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6421 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6481 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     6541 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     6601 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     6661 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     6721 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     6781 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     6841 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     6901 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     6961 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7021 ctgtacaagt aagctagctc gagctagaat taattcgata tcaagcttat cgataatcaa
     7081 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7141 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7201 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7261 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7321 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7381 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7441 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7501 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7561 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7621 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgagac
     7681 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7741 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7801 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7861 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7921 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     7981 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     8041 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     8101 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     8161 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8221 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8281 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8341 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     8401 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     8461 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     8521 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     8581 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     8641 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8701 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8761 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8821 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8881 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8941 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9001 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9061 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     9121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9181 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9241 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9361 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9421 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     9481 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     9541 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     9601 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     9661 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     9721 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     9781 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     9841 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     9901 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     9961 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    10021 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    10081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    10201 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10261 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10381 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10441 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10501 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10561 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10621 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10681 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10741 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10801 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10861 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10921 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10981 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11041 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11101 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11161 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11221 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11281 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11341 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11401 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11461 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11521 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11581 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11641 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11701 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11761 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11821 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11881 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11941 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    12001 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    12061 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12361 gaaaagtgcc acctgac
//
