LOCUS       sequence-201628-       10340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains human kappa CL domain, for cloning of antibody VL domains 
            using SapI restriction enzyme..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-4
FEATURES             Location/Qualifiers
     source          1..10340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1072..1090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1158..1262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1263..1331,1332..2123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1481..1500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2294..2882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2783..2802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3313..3458
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3501..4538
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4536..4555)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4580..4628
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5282..5661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5662..5865
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5816..5836
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5862..5886
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5907..5916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6325..6459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6349..6368)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6403..6422
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6602..6624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7184..8973
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
       61 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
      121 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
      181 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
      241 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
      301 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
      361 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
      421 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
      481 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
      541 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
      601 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
      661 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
      721 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
      781 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
      841 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
      901 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
      961 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     1021 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
     1081 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1261 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1321 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1381 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1441 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1501 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1561 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1621 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1681 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1741 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1801 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1861 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1921 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1981 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2041 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2101 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2161 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2221 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2401 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2461 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2881 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc
     2941 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
     3001 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
     3061 gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc
     3121 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     3181 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc
     3241 cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
     3301 tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
     3361 cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg
     3421 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
     3481 atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     3541 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     3601 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     3661 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     3721 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     3781 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     3841 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     3901 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     3961 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     4021 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     4081 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     4141 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     4201 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     4261 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     4321 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     4381 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     4441 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     4501 gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg
     4561 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     4621 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     4681 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     4741 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg
     4801 ccccgtgggt tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt
     4861 attttgggcg ttgcgtgggg tcaggtccac gactggactg agcagacaga cccatggttt
     4921 ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg
     4981 ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt
     5041 cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg
     5101 ctgcaggcgc agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta
     5161 gccatattag tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     5221 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     5281 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     5341 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     5401 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     5461 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     5521 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     5581 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     5641 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     5701 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5761 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     5821 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     5881 cagatctcta gaagctgggt accgccgcca ccatggtgct gcagacccag gtgttcatca
     5941 gcctgctgct gtggatcagc ggcgcctacg gcgaagagct acagagtgag ctcttcagga
     6001 ccgtggccgc ccccagcgtg ttcatcttcc cccccagcga cgagcagctg aaaagcggca
     6061 ccgccagcgt ggtgtgcctg ctgaacaact tctaccccag ggaggccaag gtgcagtgga
     6121 aggtggacaa cgccctgcag agcggcaaca gccaggagag cgtgaccgag caggacagca
     6181 aggacagcac ctacagcctg agcagcaccc tgaccctgag caaggccgac tacgagaagc
     6241 acaaggtgta cgcctgcgag gtgacccacc agggcctgag cagccccgtg accaagagct
     6301 tcaacagggg cgagtgctga taaggatcca gacatgataa gatacattga tgagtttgga
     6361 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6421 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6481 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     6541 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     6601 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6661 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6721 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     6781 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     6841 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     6901 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     6961 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     7021 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     7081 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     7141 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     7201 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     7261 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     7321 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     7381 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     7441 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     7501 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     7561 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     7621 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     7681 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     7741 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     7801 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     7861 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     7921 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     7981 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     8041 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     8101 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     8161 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     8221 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     8281 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     8341 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     8401 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     8461 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     8521 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     8581 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     8641 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     8701 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     8761 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     8821 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
     8881 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
     8941 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
     9001 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
     9061 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
     9121 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
     9181 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
     9241 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
     9301 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
     9361 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
     9421 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
     9481 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
     9541 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
     9601 taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct
     9661 tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta
     9721 cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg
     9781 aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg cctccatcac
     9841 cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg ccctgctcta
     9901 tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg tattcaaaat
     9961 aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc ctgcgcggtg
    10021 gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct ccacgtccac
    10081 gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt cctctacccc
    10141 ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc cggcctccac
    10201 tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct gcccctcctc
    10261 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tccccctcct cctcctcccc
    10321 ctcctgcccc tcctgctcct
//
