LOCUS       sequence-201643-        7066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET driver for amplified expression in cortical neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Sadakane O, Masamizu Y, Watakabe A, Terada S, Ohtsuka M, Takaji M, 
            Mizukami H, Ozawa K, Kawasaki H, Matsuzaki M, Yamamori T
  TITLE     Long-Term Two-Photon Calcium Imaging of Neuronal Populations with 
            Subcellular Resolution in Adult Non-human Primates.
  JOURNAL   Cell Rep. 2015 Dec 1;13(9):1989-99. doi: 
            10.1016/j.celrep.2015.10.050. Epub 2015 Nov 19.
  PUBMED    26655910
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.10.050"; journalName: "Cell Rep"; date: 
            "2015-12-1- 1"; volume: "13"; issue: "9"; pages: "1989-99"
COMMENT     Sequence Label: pAAV_Thy1StTA
FEATURES             Location/Qualifiers
     source          1..7066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   61..190
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             join(3112..3729,3730..3735,3736..3771,3772..3774,3775..3810,
                     3811..3813,3814..3849,3850..3858)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    3873..4349
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4072..4091)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4389..4529
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4389..4518
                     /label=AAV2 ITR
     rep_origin      4604..5059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4691..4710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4901..4922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5076..5095)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5195..5217
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5255..5273)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5341..5445
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5446..5514,5515..6306)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5664..5683)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6477..7065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6966..6985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
       61 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
      121 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      181 aggggttcct gcggccgcac gcgtggttat ccaggcttct gaaggttcaa gcaaagaaag
      241 ggttacaacc ttaaaaggag agcgtcccgg ggtatgggta gaagactgct ccaccccgac
      301 ccccagggtc cctaaccgtc ttttccctgg gcgagtcagc ccaatcacag gactgagagt
      361 gcctctttag tagcagcaag ccacttcgga cacccaaatg gaacacctcc agtcagccct
      421 cgccgaccac cccaccccct ccatcctttt ccctcagcct ccgattggct gaatctagag
      481 tccctccctg ctcccccctc tctccccacc cctggtgaaa actgcgggct tcagcgctgg
      541 gtgcagcaac tggaggcgtt ggcgcaccag gaggaggctg cagctagggg agtccaggtg
      601 agagcaggcc gacgggaggg acccgcacat gcaaggaccg ccgcagggcg aggatgcaag
      661 ccttccccag ctacagtttt gggaaaggat accagggcgc tcctatatgg gggcgcggga
      721 actggggaaa gaaggtgctc ccaggtcgag gtgggagagg aaggcagtgc ggggtcacgg
      781 gctttctccc tgctaacgga cgctttcgaa gagtgggtgc cggaggagaa ccatgaggaa
      841 ggacatcaag gacagccttt ggtccccaag ctcaaatcgc tttagtggtg cgaatagagg
      901 gaggaggtgg gtggcaaact ggagggagtc cccagcgggt gacctcgtgg ctggctgggt
      961 gcggggcacc gcaggtaaga aaaccgcaat gttgcgggag gggactgggt ggcaggcgcg
     1021 ggggagggga aagctagaaa ggatgcgagg gagcggaggg gggagggagc gggagaatct
     1081 caactggtag aggaagatta aaatgaggaa atagcatcag ggtggggtta gccaagccgg
     1141 gcctcaggga aagggcgcaa agtttgtctg ggtgtgggct taggtgggct gggtatgaga
     1201 ttcggggcgc cgaaaacact gctgcgcctc tgccaaatca cgctacccct gtatctagtt
     1261 ctgccaggct tctccagccc cagccccaat tcttttctct agtgttcccc cttccctccc
     1321 ctgaatctca agcccacact ccctcctcca taacccactg ttatcaaatc taagtcattt
     1381 gccacccaac aaccatcagg aggcggaagc agacgggagg agtttgagat caacttgggc
     1441 tacatcacga gttccaggct caccaaggct tcttaaggag accttgtctc taaaattaat
     1501 taattaatta attaatagtc ccctttctct gccacagaac cttgggatct ggctcctggt
     1561 cgcagctccc cccaccccag gctgacattc actgccatag cccatccgga aatcctagtc
     1621 tatttcccca tggatcttga actgcagaga gaatggcaga gtggcccgcc ctgtgcaaag
     1681 gatgttccta gcctaggtgg agctcgcgaa ctcgcagact gtgcctctct tgggcaagga
     1741 caggctagac agcctgccgg tgtgttgagc tagggcactg tggggaaggc agagaacctg
     1801 tgcagggcag caatgaacac aggaccagaa aactgcagcc ctaggaacac tcaagagctg
     1861 gccatttgca agcatctctg gcctccgtgc ttctcactca tgtcccatgt cttatacagg
     1921 cctctgtggc acctcgcttg cctgatctca tccctagccg ttaagctttc tgcatgactt
     1981 atcacttggg gcataatgct ggatacctac cattttctta gaccccatca aaatcctatt
     2041 tgagtgtacg gttcggagaa cctcatttat ccggtaaatg tcttttactc tgctctcagg
     2101 gagctgaggc aggacatcct gagatacatt gggagaggag atacagtttc aataaaataa
     2161 taggttgggt ggaggtacat gcctataatg ccaccactca ggaaatggtg gcagcttcgt
     2221 gagtttgagg ccaacccaag aaacatagtg aaaccctgtc agtaaataag taagcaagta
     2281 tttgagtatc tactatatgc tagggctgac ctggacatta ggggtcatct tctgaacaaa
     2341 ctagtgcttg agggaggtat ttggggtttt tgtttgttta atggatctga atgagttcca
     2401 gagactggct acacagcgat atgactgagc ttaacacccc taaagcatac agtcagacca
     2461 attagacaat aaaaggtatg tatagcttac caaataaaaa aattgtattt tcaagagagt
     2521 gtctgtctgt gtagccctgg ctgttcttga actcactctg tagaccaggc tggcctggaa
     2581 atccatctgc ctgcctctgc ctctctgcct ctctgcctct ctgcctctct ctctgcctct
     2641 ctctgcctct ctctgcccct ctctgcccct ctctgcccct ctctgccgcc ctctgccttt
     2701 tgccctctgc cctctgttct ctggcctctg ccctctgccc tctggcctct ggcctctgcc
     2761 tctgcctctt gagtgctgga atcaaaggtg tgagctctgt aggtcttaag ttccagaaga
     2821 aagtaatgaa gtcacccagc agggaggtgc tcagggacag cacagacaca cacccaggac
     2881 ataggctccc acttccttgg ctttctctga gtggcaaagg accttaggca gtgtcactcc
     2941 ctaagagaag gggataaaga gaggggctga ggtattcatc atgtgctccg tggatctcaa
     3001 gccctcaagg taaatgggga cccacctgtc ctaccagctg gctgacctgt agctttcccc
     3061 accacagaat ccaagtcgga actcttggca ccatcgattg aattcgccac catgtctaga
     3121 ctggacaaga gcaaagtcat aaactctgct ctggaattac tcaatgaagt cggtatcgaa
     3181 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3241 cacgtgaaga acaagcgggc cctgctcgat gccctggcaa tcgagatgct ggacaggcat
     3301 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3361 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3421 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3481 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3541 tgcgtattgg aggatcagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3601 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccatca gggagccgaa
     3661 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     3721 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     3781 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     3841 gacatgctcc ccgggtaact cgagagatct acgggtggca tccctgtgac ccctccccag
     3901 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     3961 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     4021 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     4081 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     4141 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     4201 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     4261 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     4321 cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga
     4381 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4441 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4501 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4561 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4681 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4801 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4921 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4981 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5041 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5101 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5161 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5221 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5281 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5341 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5401 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5461 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5521 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5581 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5641 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5701 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5761 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5821 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5881 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5941 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6001 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6061 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6121 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6181 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6241 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6301 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6361 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6421 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6481 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6541 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6601 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6661 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6721 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6781 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6841 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7021 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaa
//
