LOCUS       sequence-20189-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pYPet-His
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..81)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccggc caccttggcc
       61 ttagtggtgg tggtggtggt gtttgtacaa ttcattcata ccctcggtaa taccagcagc
      121 agtcaaaaat tctaacaaga ccatgtggtc tctcttttcg tttggatctt tgaataaggc
      181 agattgatag gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt
      241 attttgttga taatggtcag ctaattgaac accaccatct tcaatgttgt gtctaatttt
      301 gaagttagct ttgataccat tcttttgttt gtcagcagtg atgtaaacat tgtgagagtt
      361 atagttgtat tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt
      421 taattcgatt ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta
      481 gttaccgtca tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga
      541 cttgaaaaag tcatgttgtt tcatatgatc tgggtatcta gcaaaacatt gaacaccata
      601 acctaaagta gtgactaagg ttggccatgg aactggcaat ttaccagtag tacagagtaa
      661 ttttaaggtc aatttaccgt acgtagcatc accttcacct tcaccggaga cagaaaattt
      721 gtgaccatta acatcaccat ctaattcaac caaaattg
//
