LOCUS       sequence-202028-       10428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. 
            No selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Poliner E, Takeuchi T, Du ZY, Benning C, Farre EM
  TITLE     Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR 
            System in the Oleaginous Microalga, Nannochloropsis oceanica 
            CCMP1779.
  JOURNAL   ACS Synth Biol. 2018 Apr 20;7(4):962-968. doi: 
            10.1021/acssynbio.7b00362. Epub 2018 Mar 14.
  PUBMED    29518315
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00362"; journalName: "ACS Synth Biol"; date: 
            "2018-04-20- 20"; volume: "7"; issue: "4"; pages: "962-968"
COMMENT     Sequence Label: pNOC-CRISPR-35S
FEATURES             Location/Qualifiers
     source          1..10428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..520
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        557..661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(662..730,731..1522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(880..899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1693..2281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2182..2201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      2620..2629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3310..3330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3335..3344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3346..3372)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3925..8025)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3925..8025)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3925..8025)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8026..8046)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8267..8278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_RNA        9269..9344
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           9349..9416
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 accggtttct tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga
       61 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt
      121 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt
      181 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
      241 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt
      301 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagta
      361 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac
      421 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata
      481 ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac
      541 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      601 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      661 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      721 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      781 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      841 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      901 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      961 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1021 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1081 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1141 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1201 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1261 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1321 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1381 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1441 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1501 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1561 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2341 gttctttcct gcgttatccc ctgattctgt ggtttaaacc aggtcactgg attttggttt
     2401 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     2461 tcttatatgc tcaacacatg agcgaaaccc tataagaacc ctaattccct tatctgggaa
     2521 ctactcacac attattctgg agaaaaatag agagagatag atttgtagag agagactggt
     2581 gatttttgcg gactccggtc ggcatctact actagtgcgg ccgccatggc ctctagtgga
     2641 tcagcttgca tgcctgcagg agacgatatc acctcttctg tttccacgat aaaaatagac
     2701 tgctcatttc ttcgtcgtct tcatcgtctg ctttttctgc ttcgcctctg tctggggtct
     2761 gaaaccacta cacacacaca caacactcgt actcccactt tcacaaaagc gtaagctcac
     2821 cggcttttct tacacgtaca ttttagtgga tcccatcacg ccactaccac gcccgcgggg
     2881 gatggaacgg aggggagaga gagaaggggg aagcatggat gaatgagaca ttgagggaaa
     2941 ggaggggagg gagcagtcca tcagggcgct acctctcttg tcccccaaac cctgttgagc
     3001 cgttcaacat gtttcatgtt tcctccctcc ccccttccct ccctggcctt tccgcggagc
     3061 cattcaagtg acgtctggac cgcaccgtaa caaaatcgtt tctatggggg gtttgtttga
     3121 caaccacgtc ttcagcgttt ttaaaaaaaa aagcgggcaa gccctctcac cctcactcat
     3181 gcccatcctc ctcctctcct gcggaacatt cttacaaaag gcgtaactcg acgacaactc
     3241 aaagaacgac aaacatcaat cccaaaaaaa aaatctctac tcgtctctct tggatctttc
     3301 caattgtcag accttcctct tctttttcgg gctagccacc atggacgcgt agtcgggcac
     3361 gtcgtagggg tatccggcca ggatgcgctc gcacaggcgc cagccggtta ccccgttgat
     3421 ggtcacgcgg aacaacagcg agccatccgg gttgatcagc cgctcgtcaa tgatcttatt
     3481 gccgttccac agggtgccgg tcaccgtgat cttcttgccg tcgaacacgg caatgccctc
     3541 gtaaggacgg ccgaagtagt caatcatgtt cggcgtcaca ccgtcgatga ccagcgtccc
     3601 gtagtgcagt atcaccttga agtggtgatc gtccacgggg tacacaacct tgaagatctt
     3661 ctcgatctgg cccatttggt ccccgctcag gccctcgtag ggaatgatga cgtggatgtc
     3721 gatcttcagg ccgttctcgc cgctcaggac gatgcgctgg attggggtca cggagacgcc
     3781 caggttctgg aaaagcgagg acacaccgcc ctgctccagc acctggtcga ggttatagcc
     3841 agctgtctgc cgccagtcgc ccacgaagtc ctcgagagta aacaccatgt taacggaccc
     3901 gctgccggac ccgtcagccc tgctgtctcc accgagctga gagaggtcga ttcttgtttc
     3961 atagagcccc gtaattgact gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt
     4021 gtaccgcttt ctgtctatgg tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt
     4081 ggtcagagta aacaagtgga taatgttttc tgcctgctcc ctgatgggct tatccctgtg
     4141 cttattgtaa gcagaaagca ccttatcgag gttagcgtcg gcgaggatca ctcttttgga
     4201 gaattcgctt atttgctcga tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag
     4261 ctgcttctgc tcattatctt cgggagaccc tttgagcttt tcatagtggc tggccagata
     4321 caagaaatta acgtatttag agggcagtgc cagctcgtta cctttctgca gctcgcccgc
     4381 actagcgagc attcgtttcc ggccgttttc aagctcaaag agagagtact tgggaagctt
     4441 aatgatgagg tcttttttga cctctttata tcctttcgcc tcgagaaagt cgatggggtt
     4501 tttttcgaag cttgatcgct ccatgattgt gatgcccagc agttccttga cgcttttgag
     4561 ttttttagac ttccctttct ccactttggc cacaaccagt acactgtaag cgactgtagg
     4621 agaatcgaat ccgccgtatt tcttggggtc ccaatctttt ttgcgtgcga tcagcttgtc
     4681 gctgttcctt ttcgggagga tactttcctt ggagaagcct ccggtctgta cttcggtctt
     4741 tttaacgatg ttcacctgcg gcatggacag gaccttccgg actgtcgcga aatccctacc
     4801 cttgtcccac acgatttctc ctgtttctcc gtttgtttcg ataagtggtc gcttccgaat
     4861 ctctccattg gccagtgtaa tctcggtctt gaaaaaattc ataatattgc tgtaaaagaa
     4921 gtacttagcg gtggccttgc ctatttcctg ctcagacttt gcgatcattt tcctaacatc
     4981 gtacacttta tagtctccgt aaacaaattc agattcaagc ttgggatatt ttttgataag
     5041 tgcagtgcct accactgcat tcaggtaggc atcatgcgca tggtggtaat tgttgatctc
     5101 tctcacctta taaaactgaa agtcctttct gaaatctgag accagcttag acttcagagt
     5161 aataactttc acctctcgaa tcagtttgtc attttcatcg tacttggtgt tcatgcgtga
     5221 atcgagaatt tgggccacgt gcttggtgat ctggcgtgtc tcaacaagct gccttttgat
     5281 gaagccggct ttatccaact cagacaggcc acctcgttca gccttagtca gattatcgaa
     5341 cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt tcattttctt
     5401 gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg atcttgtcaa
     5461 cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatat gatccacgtc
     5521 gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc tgccgttctg
     5581 caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg ggtgttcctt
     5641 aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc tttccctact
     5701 gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa cgatattctc
     5761 gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg tctgcagtat
     5821 tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa gactgtcccc
     5881 ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt catcatggat
     5941 caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca ggattgtctt
     6001 tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc cccatcctgt
     6061 atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag cgtaagtttt
     6121 caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga caatgtcctc
     6181 aagaatgtcc tcgttctcct cattgtccag gaagtccttg tctttaatga ttttcaggag
     6241 atcgtgatac gttcccaggg atgcgttgaa gcgatcctcc actccgctga tttcaacaga
     6301 gtcgaaacat tcaatctttt tgaaatagtc ttctttgagc tgtttcacgg taactttccg
     6361 gttcgtcttg aagaggaggt ccacgatagc tttcttctgc tctccagaca ggaatgctgg
     6421 ctttctcatc ccttctgtga cgtatttgac cttggtgagc tcgttataaa ctgtgaagta
     6481 ctcgtacagc agagagtgtt taggaagcac cttttcgtta ggcagatttt tatcaaagtt
     6541 agtcatcctt tcgatgaagg actgggcaga ggccccctta tccacgactt cctcgaagtt
     6601 ccagggagtg atggtctctt ctgatttgcg agtcatccac gcgaatctgg aatttccccg
     6661 ggcgaggggg cctacatagt agggtatccg aaatgtgagg attttctcaa tcttttccct
     6721 gttatctttc aaaaaggggt agaaatcctc ttgccgcctg aggatagcgt gcagttcgcc
     6781 caggtgaatc tggtggggga tgcttccatt gtcgaaagtg cgctgtttgc gcaacagatc
     6841 ttctctgtta agctttacca gcagctcctc ggtgccgtcc attttttcca agatgggctt
     6901 aataaatttg taaaattcct cctggcttgc tccgccgtca atgtatccgg cgtagccatt
     6961 tttagactga tcgaagaaaa tttccttgta cttctcaggc agttgctgtc tgacaagggc
     7021 cttcagcaaa gtcaagtctt ggtggtgctc atcatagcgc ttgatcatac tagcgctcag
     7081 cggagctttg gtgatctccg tgttcactcg cagaatatca ctcagcagaa tggcgtctga
     7141 caggttcttt gccgccaaaa aaaggtctgc gtactggtcg ccgatctggg ccagcagatt
     7201 gtcgagatca tcatcgtagg tgtctttgct cagttgaagc ttggcatctt cggccaggtc
     7261 gaagttagat ttaaagttgg gggtcagccc gagtgacagg gcgataagat taccaaacag
     7321 gccgttcttc ttctccccag ggagctgtgc gatgaggttt tcgagccgcc gggatttgga
     7381 cagcctagcg ctcaggattg ctttggcgtc aactccggat gcgttgatcg ggttctcttc
     7441 gaaaagctga ttgtaagtct gaaccagttg gataaagagt ttgtcgacat cgctgttgtc
     7501 tgggttcagg tccccctcga tgaggaagtg tccccgaaat ttgatcatat gcgccagcgc
     7561 gagatagatc aaccgcaagt cagccttatc agtactgtct acaagcttct tcctcagatg
     7621 atatatggtt gggtactttt catggtacgc cacctcgtcc acgatattgc caaagattgg
     7681 gtggcgctcg tgctttttat cctcctccac caaaaaggac tcctccagcc tatggaagaa
     7741 agagtcatcc accttagcca tctcattact aaagatctcc tgcaggtagc agatccgatt
     7801 ctttctgcgg gtatatctgc gccgtgctgt tcttttgagc cgcgtggctt cggccgtctc
     7861 cccggagtcg aacaggaggg cgccaatgag gttcttcttt atgctgtggc gatcggtatt
     7921 gcccagaact ttgaattttt tgctcggcac cttgtactcg tccgtaatga cggcccagcc
     7981 gacgctgttt gtgccgatat cgagcccaat ggagtacttc ttgtccacct tccgcttttt
     8041 cttgggcatg ctcgagggtt gcgtgtgtat ctgtgtgcag tggatattgt taccgagttt
     8101 ggtgagcgtg agtccgttag tgccctggtg gtggtggatt aggagagtgg gtgactcggt
     8161 gtccatggct ttcttcgctc attataggag gggaaaggaa tgagggaggg tggggagacc
     8221 gcgtctgttg ttgaccaccg atttacttct tgcctccctt cccctccctc ccctcaatcc
     8281 gtacgacaca aatagtagcc gagtgtctgc tgcagagcgc atgattagtg tggtagacaa
     8341 cgagggaggg aaggatgtac agggcatggc acggagaagc gatggtggcc aggaagagga
     8401 gaggtcgcga gaacaggatg tgttgcgaat ggataaaaac agaaagcgat ggctctgggc
     8461 ttcgaaagca ggggacatta ggacgtgtag accatctcga cggatccctc tgtatctctg
     8521 ttgtgcgtga atgttttctg tgcacgtgta gtgtgtgaga gtagaacccg ggaactcgaa
     8581 cagagaaaag catgggtggc tgtggtgtgg aggcttcgtt cccaccacat gcccttctcc
     8641 ttcgcctcgc ctctccctgc cttcttccac gcacccttgc gcccctcgtt ctcaatacct
     8701 ggctcacttc caccattcaa acaaccatca cgatacaggc atttatctat cgttgaagac
     8761 ttcttcctcc ggtagatctt agccaaggta agaagagggg catgcagcaa ggagaaagaa
     8821 atgatgcatg atgaggaata gaaggggagg agggagggat atgatgggaa gcgaaagcgc
     8881 atattctggt ggtctgctgc ctgatgggga cgcgtctagc tgtgacactg aggacggtgg
     8941 ctgctggtgg ctgcgggcgc tgccttggtg atcaatggga gtaaagggag ggaaggaggt
     9001 agcgtgaacg gatgacgcgg agaagtttag gggtctcttt acgtatcgcc cctgccgccc
     9061 gcctctctgc gataaatgtg cctgttaccc tgcagcctct attcttcact gtgttcctgt
     9121 tttccaacag cctctattct tccctgtctt ttgttgcagt ggcgtcatcc tctctttgcc
     9181 ccagtcgtcg ttctctcgac tcactcactc cccccctcct tccctccctc catccacaga
     9241 atcgatgaag agcaggcctt ggctcttcgt tttagagcta gaaatagcaa gttaaaataa
     9301 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcttttgg ccggcatggt
     9361 cccagcctcc tcgctggcgc cggctgggca acatgcttcg gcatggcgaa tgggactcct
     9421 gggtaccatg ggaaagaaag gatgagaaag gagaaaggac atctagataa ccggcatatc
     9481 acggtggtgt attagtgtag aatagtgaag agaagacttg ggaaaatgtg taggaaaggt
     9541 tgtttctgtg tatgtgggtt gggatgggtg gctgtttgag aaggaacagc gggcagggcg
     9601 atgtagtgct gaacgggcac ggaaccactg agactgaagg aagtagggag agagaggggc
     9661 aggggacgtg cactttaatc tttgcctcgg tagagtatac ccatgcaaga gtatgtggcc
     9721 acctgtggtg gcttttggcc aggtctggtg cagtgccaat catctcccat caataataca
     9781 acttcagaac aacggcgcat tgatggggag agagaaagta aatttaagta aggggtacgt
     9841 agtagaggat tcaactgaaa tattttcgag gagcggttgg gaagttgacc gattgaaagg
     9901 agaagggagg ggagcaggtg tgatagtcat gtgtaaagta attctttttt gccgtcgtca
     9961 cacaatccac atcaatgata aaatatgttt aaggatcaat cacacggagt cggtcataag
    10021 gcaaccgcaa acgcaatgca aactagcaag caagcaggac cacaacaaca accattacca
    10081 tcacaggcga cagcagcagc agctgcagca gcagcaaggc aagcaaaggc catctgcgcg
    10141 tggacttttc caggcaccgg ctttaagcgt aattttcaat gattgttgtc cgtgtatcct
    10201 ctcacccttt caccgcttgc gcgcacgtga gcagcacaac tggtaatgcc cgaggacaat
    10261 agacgaggaa aaagaagaag aaaaatgaag agggagtgat gaagaagaag agaagaataa
    10321 aagaagacag aaaagaagat aaaaaagtca aaagacgaca aagacggaaa aaaaagaaac
    10381 gcgcaatttt aacaccagca acgacgacga aaagggcgtc agctttgc
//
