LOCUS       sequence-202062-       11463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING delta 368 and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STING 368STOP
FEATURES             Location/Qualifiers
     source          1..11463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          48..113
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             243..263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    688..822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(725..744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     779..798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             990..1623
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1670..1795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2288..2521
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2706..2750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3043..3160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3300..3707
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3726..4424
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4434..4490
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5872..6052
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7580..7599)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7813..7832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7932..7954
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7992..8010)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8078..8182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8183..8251,8252..9043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8401..8420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9214..9802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9703..9722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9956..9973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10043..10063)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10048..10377
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10228..10363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
       61 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
      121 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
      181 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
      241 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
      301 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
      361 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
      421 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
      481 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
      541 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
      601 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
      661 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      781 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
      841 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
      901 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
      961 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     1021 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     1081 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     1141 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     1201 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     1261 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     1321 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     1381 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     1441 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1501 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1561 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1621 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1681 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1741 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1801 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1861 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1921 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1981 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     2041 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     2101 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     2161 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     2221 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     2281 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     2341 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     2401 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     2461 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2521 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2581 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2641 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2701 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2761 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2821 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2881 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2941 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     3001 gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3061 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     3181 cagagatcca ctttggctga tacgcgtctg cagccccgat aaaataaaag attttattta
     3241 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgcag
     3301 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
     3361 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
     3421 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
     3481 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
     3541 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
     3601 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
     3661 acccctcact cggcgcgcca gtcctccgac agactgagtc gcccgggccg cgggctagcg
     3721 ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     3781 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     3841 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     3901 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     3961 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     4021 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     4081 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     4141 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     4201 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     4261 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     4321 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     4381 ccagatactg cgacctccct agcaaactgg ggcacaagct taatggaagc ggagctacta
     4441 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct ggatccatgc
     4501 cccactccag cctgcatcca tccatcccgt gtcccagggg tcacggggcc cagaaggcag
     4561 ccttggttct gctgagtgcc tgcctggtga ccctttgggg gctaggagag ccaccagagc
     4621 acactctccg gtacctggtc ctccacctag cctccctgca gctgggactg ctgttaaacg
     4681 gggtctgcag cctggctgag gagctgcgcc acatccactc caggtaccgg ggcagctact
     4741 ggaggactgt gcgggcctgc ctgggctgcc ccctccgccg tggggccctg ttgctgctgt
     4801 ccatctattt ctactactcc ctcccaaatg cggtcggccc gcccttcact tggatgcttg
     4861 ccctcctggg cctctcgcag gcactgaaca tcctcctggg cctcaagggc ctggccccag
     4921 ctgagatctc tgcagtgtgt gaaaaaggga atttcaacgt ggcccatggg ctggcatggt
     4981 catattacat cggatatctg cggctgatcc tgccagagct ccaggcccgg attcgaactt
     5041 acaatcagca ttacaacaac ctgctacggg gtgcagtgag ccagcggctg tatattctcc
     5101 tcccattgga ctgtggggtg cctgataacc tgagtatggc tgaccccaac attcgcttcc
     5161 tggataaact gccccagcag accggtgacc gtgctggcat caaggatcgg gtttacagca
     5221 acagcatcta tgagcttctg gagaacgggc agcgggcggg cacctgtgtc ctggagtacg
     5281 ccaccccctt gcagactttg tttgccatgt cacaatacag tcaagctggc tttagccggg
     5341 aggataggct tgagcaggcc aaactcttct gccggacact tgaggacatc ctggcagatg
     5401 cccctgagtc tcagaacaac tgccgcctca ttgcctacca ggaacctgca gatgacagca
     5461 gcttctcgct gtcccaggag gttctccggc acctgcggca ggaggaaaag gaagaggtta
     5521 ctgtgggcag cttgaagacc tcagcggtgc ccagtacctc cacgatgtcc caagagcctg
     5581 agctcctcat cagtggaatg tgactcgaga cctagaaaaa catggagcaa tcacaagtag
     5641 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     5701 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5761 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5821 acaaaatcgt cgagagatgc tgcatataag cagctgcttt ttgcttgtac tgggtctctc
     5881 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5941 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6001 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6061 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6121 aggccttgac attataatag atttagcagg aattgaacta ggagtggagc acacaggcaa
     6181 agctgcagaa gtacttggaa gaagccacca gagatactca cgattctgca catacctggc
     6241 taatcccaga tcctaaggat tacattaagt ttactaacat ttatataatg atttatagtt
     6301 taaagtataa acttatctaa tttactattc tgacagatat taattaatcc tcaaatatca
     6361 taagagatga ttactattat ccccatttaa cacaagagga aactgagagg gaaagatgtt
     6421 gaagtaattt tcccacaatt acagcatccg ttagttacga ctctatgatc ttctgacaca
     6481 aattccattt actcctcacc ctatgactca gtcgaatata tcaaagttat ggacattatg
     6541 ctaagtaaca aattaccctt ttatatagta aatactgagt agattgagag aagaaattgt
     6601 ttgcaaacct gaatagcttc aagaagaaga gaagtgagga taagaataac agttgtcatt
     6661 taacaagttt taacaagtaa cttggttaga aagggattca aatgcataaa gcaagggata
     6721 aatttttctg gcaacaagac tatacaatat aaccttaaat atgacttcaa ataattgttg
     6781 gaacttgata aaactaatta aatattattg aagattatca atattataaa tgtaatttac
     6841 ttttaaaaag ggaacataga aatgtgtatc attagagtag aaaacaatcc ttattatcac
     6901 aatttgtcaa aacaagtttg ttattaacac aagtagaata ctgcattcaa ttaagttgac
     6961 tgcagatttt gtgttttgtt aaaattagaa agagataaca acaatttgaa ttattgaaag
     7021 taacatgtaa atagttctac atacgttctt ttgacatctt gttcaatcat tgatcgaagt
     7081 tctttatctt ggaagaattt gttccaaaga ctctgaaata aggaaaacaa tctattatat
     7141 agtctcacac ctttgtttta cttttagtga tttcaattta ataatgtaaa tggttaaaat
     7201 ttattcttct ctgagatcat ttcacattgc agatagaaaa cctgagactg gggtaatttt
     7261 tattaaaatc taatttaatc tcagaaacac atctttattc taacatcaat ttttccagtt
     7321 tgatattatc atataaagtc agccttcctc atctgcaggt tccacaacaa aaatccaacc
     7381 aactgtggat caaaaatatt gggaaaaaat taaaaatagc aatacaacaa taaaaaaata
     7441 caaatcagaa aaacagcaca gtataacaac tttatttagc atttacaatc tattaggtat
     7501 tataagtaat ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     7561 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     7621 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     7681 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     7741 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     7801 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     7861 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7921 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7981 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     8041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8161 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8221 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8281 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8341 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8401 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8461 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8521 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8581 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8641 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8701 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8761 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8821 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8881 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8941 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9001 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9061 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9361 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9601 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9781 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9841 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9901 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9961 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    10021 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    10081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    10141 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    10201 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10261 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10321 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10381 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
    10441 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
    10501 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
    10561 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
    10621 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
    10681 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    10741 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
    10801 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    10861 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
    10921 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
    10981 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    11041 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
    11101 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    11161 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    11221 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    11281 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
    11341 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
    11401 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
    11461 gtg
//
