LOCUS       sequence-202064-       11496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING V155M HAQ and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          55..120
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             250..270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    695..829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(732..751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     786..805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             997..1630
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1677..1802
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2295..2528
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2713..2757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3050..3167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3307..3714
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3733..4431
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4441..4497
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5912..6092
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7620..7639)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7853..7872)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7972..7994
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8032..8050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8118..8222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8223..8291,8292..9083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8441..8460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9254..9842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9743..9762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9996..10013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10083..10103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10088..10417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10268..10403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
       61 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
      121 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
      181 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
      241 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
      301 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
      361 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
      421 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
      481 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
      541 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
      601 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      661 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
      841 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
      901 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
      961 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     1021 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     1081 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     1141 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     1201 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     1261 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     1321 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     1381 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     1441 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     1501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     1561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1621 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     1681 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1741 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1801 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1861 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1921 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1981 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     2041 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     2101 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     2161 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     2221 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     2281 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     2341 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     2401 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     2461 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     2521 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     2581 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2641 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2701 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2761 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2821 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2881 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2941 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     3001 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa
     3061 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3121 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     3181 gggacagcag agatccactt tggctgatac gcgtctgcag ccccgataaa ataaaagatt
     3241 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     3301 gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc
     3361 agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga
     3421 gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc
     3481 cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc
     3541 atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa
     3601 ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga
     3661 gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc cgggccgcgg
     3721 gctagcgcca ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     3781 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     3841 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     3901 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     3961 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     4021 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     4081 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     4141 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     4201 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     4261 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     4321 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     4381 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tggaagcgga
     4441 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctgga
     4501 tccatgcccc actccagcct gcatccatcc atcccgtgtc ccaggggtca cggggcccag
     4561 aaggcagcct tggttctgct gagtgcctgc ctggtgaccc tttgggggct aggagagcca
     4621 ccagagcaca ctctccggta cctggtgctc cacctagcct ccctgcagct gggactgctg
     4681 ttaaacgggg tctgcagcct ggctgaggag ctgcaccaca tccactccag gtaccggggc
     4741 agctactgga ggactgtgcg ggcctgcctg ggctgccccc tccgccgtgg ggccctgttg
     4801 ctgctgtcca tctatttcta ctactccctc ccaaatgcgg tcggcccgcc cttcacttgg
     4861 atgcttgccc tcctgggcct ctcgcaggca ctgaacatcc tcctgggcct caagggcctg
     4921 gccccagctg agatctctgc agtgtgtgaa aaagggaatt tcaacatggc ccatgggctg
     4981 gcatggtcat attacatcgg atatctgcgg ctgatcctgc cagagctcca ggcccggatt
     5041 cgaacttaca atcagcatta caacaacctg ctacggggtg cagtgagcca gcggctgtat
     5101 attctcctcc cattggactg tggggtgcct gataacctga gtatggctga ccccaacatt
     5161 cgcttcctgg ataaactgcc ccagcagacc gctgaccgtg ctggcatcaa ggatcgggtt
     5221 tacagcaaca gcatctatga gcttctggag aacgggcagc gggcgggcac ctgtgtcctg
     5281 gagtacgcca cccccttgca gactttgttt gccatgtcac aatacagtca agctggcttt
     5341 agccgggagg ataggcttga gcaggccaaa ctcttctgcc agacacttga ggacatcctg
     5401 gcagatgccc ctgagtctca gaacaactgc cgcctcattg cctaccagga acctgcagat
     5461 gacagcagct tctcgctgtc ccaggaggtt ctccggcacc tgcggcagga ggaaaaggaa
     5521 gaggttactg tgggcagctt gaagacctca gcggtgccca gtacctccac gatgtcccaa
     5581 gagcctgagc tcctcatcag tggaatggaa aagcccctcc ctctccgcac ggatttctct
     5641 tgactcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     5701 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5761 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5821 aaaagggggg actggaaggg ctaattcact cccaacgaag acaaaatcgt cgagagatgc
     5881 tgcatataag cagctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5941 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6001 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6061 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6121 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attataatag
     6181 atttagcagg aattgaacta ggagtggagc acacaggcaa agctgcagaa gtacttggaa
     6241 gaagccacca gagatactca cgattctgca catacctggc taatcccaga tcctaaggat
     6301 tacattaagt ttactaacat ttatataatg atttatagtt taaagtataa acttatctaa
     6361 tttactattc tgacagatat taattaatcc tcaaatatca taagagatga ttactattat
     6421 ccccatttaa cacaagagga aactgagagg gaaagatgtt gaagtaattt tcccacaatt
     6481 acagcatccg ttagttacga ctctatgatc ttctgacaca aattccattt actcctcacc
     6541 ctatgactca gtcgaatata tcaaagttat ggacattatg ctaagtaaca aattaccctt
     6601 ttatatagta aatactgagt agattgagag aagaaattgt ttgcaaacct gaatagcttc
     6661 aagaagaaga gaagtgagga taagaataac agttgtcatt taacaagttt taacaagtaa
     6721 cttggttaga aagggattca aatgcataaa gcaagggata aatttttctg gcaacaagac
     6781 tatacaatat aaccttaaat atgacttcaa ataattgttg gaacttgata aaactaatta
     6841 aatattattg aagattatca atattataaa tgtaatttac ttttaaaaag ggaacataga
     6901 aatgtgtatc attagagtag aaaacaatcc ttattatcac aatttgtcaa aacaagtttg
     6961 ttattaacac aagtagaata ctgcattcaa ttaagttgac tgcagatttt gtgttttgtt
     7021 aaaattagaa agagataaca acaatttgaa ttattgaaag taacatgtaa atagttctac
     7081 atacgttctt ttgacatctt gttcaatcat tgatcgaagt tctttatctt ggaagaattt
     7141 gttccaaaga ctctgaaata aggaaaacaa tctattatat agtctcacac ctttgtttta
     7201 cttttagtga tttcaattta ataatgtaaa tggttaaaat ttattcttct ctgagatcat
     7261 ttcacattgc agatagaaaa cctgagactg gggtaatttt tattaaaatc taatttaatc
     7321 tcagaaacac atctttattc taacatcaat ttttccagtt tgatattatc atataaagtc
     7381 agccttcctc atctgcaggt tccacaacaa aaatccaacc aactgtggat caaaaatatt
     7441 gggaaaaaat taaaaatagc aatacaacaa taaaaaaata caaatcagaa aaacagcaca
     7501 gtataacaac tttatttagc atttacaatc tattaggtat tataagtaat ctagccagat
     7561 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     7621 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     7681 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     7741 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     7801 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     7861 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7921 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7981 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     8041 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     8101 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8161 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8221 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8281 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8341 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8401 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8461 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8521 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8581 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8641 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8701 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8761 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8821 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8881 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8941 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9001 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9061 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9121 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9181 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9421 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10021 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    10081 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10141 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10201 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10261 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10321 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10381 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
    10441 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
    10501 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
    10561 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
    10621 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
    10681 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
    10741 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
    10801 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
    10861 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
    10921 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
    10981 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
    11041 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
    11101 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
    11161 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
    11221 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
    11281 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
    11341 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
    11401 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
    11461 atgagtgggc cccggatctt tgtgaaggaa ccttac
//
