LOCUS       sequence-202065-       11385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING V155M delta 342 and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP
FEATURES             Location/Qualifiers
     source          1..11385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          45..110
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             240..260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    685..819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(722..741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     776..795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             987..1620
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1667..1792
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2285..2518
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2703..2747
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3040..3157
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3297..3704
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3723..4421
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4431..4487
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5791..5971
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7499..7518)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7732..7751)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7851..7873
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7911..7929)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7997..8101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8102..8170,8171..8962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8320..8339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9133..9721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9622..9641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9875..9892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9962..9982)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9967..10296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10147..10282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
       61 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
      121 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
      181 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
      241 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
      301 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
      361 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
      421 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
      481 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
      541 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
      601 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      661 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      781 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
      841 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
      901 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
      961 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     1021 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     1081 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     1141 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     1201 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     1261 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     1321 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     1381 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     1441 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1501 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1561 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1621 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1681 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1741 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1801 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1861 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1921 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1981 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     2041 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     2101 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     2161 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     2221 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     2281 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     2341 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     2401 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     2461 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     2521 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     2581 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2641 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2701 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2761 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2821 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2881 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2941 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     3001 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     3061 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3121 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     3181 agatccactt tggctgatac gcgtctgcag ccccgataaa ataaaagatt ttatttagtc
     3241 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa
     3301 cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg
     3361 cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg
     3421 ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag
     3481 aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt
     3541 tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc
     3601 tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc
     3661 cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc cgggccgcgg gctagcgcca
     3721 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     3781 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     3841 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     3901 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     3961 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     4021 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     4081 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     4141 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     4201 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat
     4261 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     4321 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     4381 gatactgcga cctccctagc aaactggggc acaagcttaa tggaagcgga gctactaact
     4441 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctgga tccatgcccc
     4501 actccagcct gcatccatcc atcccgtgtc ccaggggtca cggggcccag aaggcagcct
     4561 tggttctgct gagtgcctgc ctggtgaccc tttgggggct aggagagcca ccagagcaca
     4621 ctctccggta cctggtcctc cacctagcct ccctgcagct gggactgctg ttaaacgggg
     4681 tctgcagcct ggctgaggag ctgcgccaca tccactccag gtaccggggc agctactgga
     4741 ggactgtgcg ggcctgcctg ggctgccccc tccgccgtgg ggccctgttg ctgctgtcca
     4801 tctatttcta ctactccctc ccaaatgcgg tcggcccgcc cttcacttgg atgcttgccc
     4861 tcctgggcct ctcgcaggca ctgaacatcc tcctgggcct caagggcctg gccccagctg
     4921 agatctctgc agtgtgtgaa aaagggaatt tcaacatggc ccatgggctg gcatggtcat
     4981 attacatcgg atatctgcgg ctgatcctgc cagagctcca ggcccggatt cgaacttaca
     5041 atcagcatta caacaacctg ctacggggtg cagtgagcca gcggctgtat attctcctcc
     5101 cattggactg tggggtgcct gataacctga gtatggctga ccccaacatt cgcttcctgg
     5161 ataaactgcc ccagcagacc ggtgaccgtg ctggcatcaa ggatcgggtt tacagcaaca
     5221 gcatctatga gcttctggag aacgggcagc gggcgggcac ctgtgtcctg gagtacgcca
     5281 cccccttgca gactttgttt gccatgtcac aatacagtca agctggcttt agccgggagg
     5341 ataggcttga gcaggccaaa ctcttctgcc ggacacttga ggacatcctg gcagatgccc
     5401 ctgagtctca gaacaactgc cgcctcattg cctaccagga acctgcagat gacagcagct
     5461 tctcgctgtc ccaggaggtt ctccggcacc tgcggcagga ggaaaaggaa gaggttactt
     5521 gactcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5581 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5641 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5701 aaagggggga ctggaagggc taattcactc ccaacgaaga caaaatcgtc gagagatgct
     5761 gcatataagc agctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5941 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6001 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttataataga
     6061 tttagcagga attgaactag gagtggagca cacaggcaaa gctgcagaag tacttggaag
     6121 aagccaccag agatactcac gattctgcac atacctggct aatcccagat cctaaggatt
     6181 acattaagtt tactaacatt tatataatga tttatagttt aaagtataaa cttatctaat
     6241 ttactattct gacagatatt aattaatcct caaatatcat aagagatgat tactattatc
     6301 cccatttaac acaagaggaa actgagaggg aaagatgttg aagtaatttt cccacaatta
     6361 cagcatccgt tagttacgac tctatgatct tctgacacaa attccattta ctcctcaccc
     6421 tatgactcag tcgaatatat caaagttatg gacattatgc taagtaacaa attacccttt
     6481 tatatagtaa atactgagta gattgagaga agaaattgtt tgcaaacctg aatagcttca
     6541 agaagaagag aagtgaggat aagaataaca gttgtcattt aacaagtttt aacaagtaac
     6601 ttggttagaa agggattcaa atgcataaag caagggataa atttttctgg caacaagact
     6661 atacaatata accttaaata tgacttcaaa taattgttgg aacttgataa aactaattaa
     6721 atattattga agattatcaa tattataaat gtaatttact tttaaaaagg gaacatagaa
     6781 atgtgtatca ttagagtaga aaacaatcct tattatcaca atttgtcaaa acaagtttgt
     6841 tattaacaca agtagaatac tgcattcaat taagttgact gcagattttg tgttttgtta
     6901 aaattagaaa gagataacaa caatttgaat tattgaaagt aacatgtaaa tagttctaca
     6961 tacgttcttt tgacatcttg ttcaatcatt gatcgaagtt ctttatcttg gaagaatttg
     7021 ttccaaagac tctgaaataa ggaaaacaat ctattatata gtctcacacc tttgttttac
     7081 ttttagtgat ttcaatttaa taatgtaaat ggttaaaatt tattcttctc tgagatcatt
     7141 tcacattgca gatagaaaac ctgagactgg ggtaattttt attaaaatct aatttaatct
     7201 cagaaacaca tctttattct aacatcaatt tttccagttt gatattatca tataaagtca
     7261 gccttcctca tctgcaggtt ccacaacaaa aatccaacca actgtggatc aaaaatattg
     7321 ggaaaaaatt aaaaatagca atacaacaat aaaaaaatac aaatcagaaa aacagcacag
     7381 tataacaact ttatttagca tttacaatct attaggtatt ataagtaatc tagccagatc
     7441 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     7501 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     7561 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     7621 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     7681 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     7741 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     7801 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7861 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     7921 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7981 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8041 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9781 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9841 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9901 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9961 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10021 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    10081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    10141 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10201 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10261 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
    10321 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
    10381 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
    10441 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
    10501 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
    10561 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
    10621 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
    10681 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
    10741 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
    10801 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
    10861 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
    10921 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
    10981 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
    11041 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
    11101 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
    11161 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
    11221 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
    11281 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
    11341 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtg
//
