LOCUS       sequence-202138-       11909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector expressing cGAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, 
            Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
  TITLE     Transmission of innate immune signaling by packaging of cGAMP in 
            viral particles.
  JOURNAL   Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
            Epub 2015 Jul 30.
  PUBMED    26229115
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab3628"; journalName: "Science"; date: 
            "2015-09-11- 11"; volume: "349"; issue: "6253"; pages: "1232-6"
COMMENT     Sequence Label: pTRIP-CMV-Puro-2A-cGAS
FEATURES             Location/Qualifiers
     source          1..11909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          58..123
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             253..273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    698..832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(735..754)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     789..808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1000..1633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1680..1805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2298..2531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2716..2760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3053..3170
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3269..3572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3573..3776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3727..3747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3816..4412
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3816..3835)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4312..4332
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4422..4478
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             6328..6508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8036..8055)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8269..8288)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8388..8410
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8448..8466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8534..8638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8639..8707,8708..9499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8857..8876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9670..10258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10159..10178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10412..10429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10499..10519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10504..10833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10684..10819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
       61 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
      121 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
      181 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
      241 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
      301 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
      361 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
      421 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
      481 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
      541 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
      601 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      661 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
      841 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
      901 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
      961 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     1021 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     1081 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     1141 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     1201 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     1261 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     1321 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     1381 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     1441 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1501 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1561 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1621 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1681 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1741 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1801 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1861 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1921 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1981 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     2041 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     2101 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     2161 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     2221 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     2281 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     2341 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     2401 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     2461 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     2521 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     2581 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2641 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2701 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2761 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2821 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2881 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2941 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     3001 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
     3061 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     3121 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     3181 acagggacag cagagatcca ctttggctga tacgcgtata gtaatcaatt acggggtcat
     3241 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     3301 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     3361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     3421 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     3481 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     3541 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     3601 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     3661 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     3721 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     3781 tagtgaaccg tcagatcccg cgggctagcg ccaccatgac cgagtacaag cccacggtgc
     3841 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     3901 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     3961 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4021 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4081 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4141 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4201 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4261 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     4321 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     4381 gcacctggtg catgacccgc aagcccggtg ccggaagcgg agctactaac ttcagcctgc
     4441 tgaagcaggc tggagacgtg gaggagaacc ctggacctgg atccactagt atgcagcctt
     4501 ggcacggaaa ggccatgcag agagcttccg aggccggagc cactgccccc aaggcttccg
     4561 cacggaatgc caggggcgcc ccgatggatc ccaacgagtc tccggctgcc cccgaggccg
     4621 ccctgcctaa ggcgggaaag ttcggccccg ccaggaagtc gggatcccgg cagaaaaaga
     4681 gcgccccgga cacccaggag aggccgcccg tccgcgcaac tggggcccgc gccaaaaagg
     4741 cccctcagcg cgcccaggac acgcagccgt ctgacgccac cagcgcccct ggggcagagg
     4801 ggctggagcc tcctgcggct cgggagccgg ctctttccag ggctggttct tgccgccaga
     4861 ggggcgcgcg ctgctccacg aagccaagac ccccgcccgg gccctgggac gtgcccagcc
     4921 ccggcctgcc ggtctcggcc cccattctcg tacggaggga tgcggcgcct ggggcctcga
     4981 agctccgggc ggttttggag aagttgaagc tcagccgcga tgatatctcc acggcggcgg
     5041 ggatggtgaa aggggttgtg gaccacctgc tgctcagact gaagtgcgac tccgcgttca
     5101 gaggcgtcgg gctgctgaac accgggagct actatgagca cgtgaagatt tctgcaccta
     5161 atgaatttga tgtcatgttt aaactggaag tccccagaat tcaactagaa gaatattcca
     5221 acactcgtgc atattacttt gtgaaattta aaagaaatcc gaaagaaaat catctgagtc
     5281 agtttttaga aggtgaaata ttatcagctt ctaagatgct gtcaaagttt aggaaaatca
     5341 ttaaggaaga aattaacgac attaaagata cagatgtcat catgaagagg aaaagaggag
     5401 ggagccctgc tgtaacactt cttattagtg aaaaaatatc tgtggatata accctggctt
     5461 tggaatcaaa aagtagctgg cctgctagca cccaagaagg cctgcgcatt caaaactggc
     5521 tttcagcaaa agttaggaag caactacgac taaagccatt ttaccttgta cccaagcatg
     5581 caaaggaagg aaatggtttc caagaagaaa catggcggct atccttctct cacatcgaaa
     5641 aggaaatttt gaacaatcat ggaaaatcta aaacgtgctg tgaaaacaaa gaagagaaat
     5701 gttgcaggaa agattgttta aaactaatga aatacctttt agaacagctg aaagaaaggt
     5761 ttaaagacaa aaaacatctg gataaattct cttcttatca tgtgaaaact gccttctttc
     5821 acgtatgtac ccagaaccct caagacagtc agtgggaccg caaagacctg ggcctctgct
     5881 ttgataactg cgtgacatac tttcttcagt gcctcaggac agaaaaactt gagaattatt
     5941 ttattcctga attcaatcta ttctctagca acttaattga caaaagaagt aaagaatttc
     6001 tgacaaagca aattgaatat gaaagaaaca atgagtttcc agtttttgat gaattttgac
     6061 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     6121 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     6181 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6241 ggggggactg gaagggctaa ttcactccca acgaagacaa aatcgtcgag agatgctgca
     6301 tataagcagc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6361 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6421 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6481 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6541 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacatta taatagattt
     6601 agcaggaatt gaactaggag tggagcacac aggcaaagct gcagaagtac ttggaagaag
     6661 ccaccagaga tactcacgat tctgcacata cctggctaat cccagatcct aaggattaca
     6721 ttaagtttac taacatttat ataatgattt atagtttaaa gtataaactt atctaattta
     6781 ctattctgac agatattaat taatcctcaa atatcataag agatgattac tattatcccc
     6841 atttaacaca agaggaaact gagagggaaa gatgttgaag taattttccc acaattacag
     6901 catccgttag ttacgactct atgatcttct gacacaaatt ccatttactc ctcaccctat
     6961 gactcagtcg aatatatcaa agttatggac attatgctaa gtaacaaatt acccttttat
     7021 atagtaaata ctgagtagat tgagagaaga aattgtttgc aaacctgaat agcttcaaga
     7081 agaagagaag tgaggataag aataacagtt gtcatttaac aagttttaac aagtaacttg
     7141 gttagaaagg gattcaaatg cataaagcaa gggataaatt tttctggcaa caagactata
     7201 caatataacc ttaaatatga cttcaaataa ttgttggaac ttgataaaac taattaaata
     7261 ttattgaaga ttatcaatat tataaatgta atttactttt aaaaagggaa catagaaatg
     7321 tgtatcatta gagtagaaaa caatccttat tatcacaatt tgtcaaaaca agtttgttat
     7381 taacacaagt agaatactgc attcaattaa gttgactgca gattttgtgt tttgttaaaa
     7441 ttagaaagag ataacaacaa tttgaattat tgaaagtaac atgtaaatag ttctacatac
     7501 gttcttttga catcttgttc aatcattgat cgaagttctt tatcttggaa gaatttgttc
     7561 caaagactct gaaataagga aaacaatcta ttatatagtc tcacaccttt gttttacttt
     7621 tagtgatttc aatttaataa tgtaaatggt taaaatttat tcttctctga gatcatttca
     7681 cattgcagat agaaaacctg agactggggt aatttttatt aaaatctaat ttaatctcag
     7741 aaacacatct ttattctaac atcaattttt ccagtttgat attatcatat aaagtcagcc
     7801 ttcctcatct gcaggttcca caacaaaaat ccaaccaact gtggatcaaa aatattggga
     7861 aaaaattaaa aatagcaata caacaataaa aaaatacaaa tcagaaaaac agcacagtat
     7921 aacaacttta tttagcattt acaatctatt aggtattata agtaatctag ccagatcctc
     7981 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     8041 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     8101 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     8161 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     8221 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     8281 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     8341 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     8401 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     8461 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8881 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10321 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10381 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10441 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
    10501 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10561 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    10621 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    10681 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    10741 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
    10801 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
    10861 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
    10921 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
    10981 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
    11041 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
    11101 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
    11161 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
    11221 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
    11281 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
    11341 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
    11401 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
    11461 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
    11521 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
    11581 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
    11641 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
    11701 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
    11761 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
    11821 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
    11881 gtgggccccg gatctttgtg aaggaacct
//
