LOCUS       sequence-20222-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKgfp2.2
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..106)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aatggtgcaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
       61 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
      121 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
      181 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
      241 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
      301 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
      361 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
      421 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
      481 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
      541 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
      601 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
      661 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
      721 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggc
//
