LOCUS       sequence-202278-        8324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Gejman RS, Chang AY, Jones HF, DiKun K, Hakimi AA, Schietinger A, 
            Scheinberg DA
  TITLE     Rejection of immunogenic tumor clones is limited by clonal fraction.
  JOURNAL   Elife. 2018 Nov 30;7. pii: 41090. doi: 10.7554/eLife.41090.
  PUBMED    30499773
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-11-30"; volume: "7. pii: 41090. doi"; pages: " 
            10.7554/eLife.41090"
COMMENT     Sequence Label: PresentER-MSIIFFLPL (GFP)
FEATURES             Location/Qualifiers
     source          1..8324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           69..181
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        195..694
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(230..253)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     627..646
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             715..1314
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(715..734)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1211..1231
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1374..1940
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1391..1942
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1535..1552)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1762..1781
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1954..1956,1957..1959,1960..2673)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1954..1956,1957..1959,1960..2670)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1999..2020)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2260..2279)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2607..2628
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2775..3289
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(3458..3474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3458..3474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3471..3493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3482..3498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3506..3512,3513..3530,3531..3536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3551..3572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3689..3706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3860..4448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3940..3959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4619..5410,5411..5479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5242..5261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5480..5584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5652..5670
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5708..5730)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5830..5849
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6043..6065
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6193..6212
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6412..6928
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6992..7333
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7400..7816
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7746..7768
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7784..7800
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           7856..7949
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aggccaaaga tcggaagagc gtcgtgtagg gaaagagtgt agatctcggt ggtcgccgta
       61 tcattgaatt caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct
      121 tgctatctct ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt
      181 ttttcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
      241 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
      301 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
      361 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
      421 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
      481 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
      541 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
      601 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
      661 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
      721 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
      781 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
      841 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
      901 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
      961 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1021 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1081 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1141 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1201 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1261 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     1321 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatcatcc aattccgccc
     1381 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     1441 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     1501 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     1561 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     1621 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     1681 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     1741 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     1801 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     1861 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     1921 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc
     1981 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2041 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2101 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     2161 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2221 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2281 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2401 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2461 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2521 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     2581 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2641 atcactctcg gcatggacga gctgtacaag taatgaatta attaagaatt atcaagctta
     2701 tcgataccgt cgacctgcag ccaagcttat cgataaaata aaagatttta tttagtctcc
     2761 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     2821 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2881 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2941 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     3001 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     3061 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     3121 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     3181 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     3241 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt
     3301 tcttgaagtt ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc
     3361 aattagccac tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca
     3421 gcgcagaaga gctggggaga attaattcgt aatcatggtc atagctgttt cctgtgtgaa
     3481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3601 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3661 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3721 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4261 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4561 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4741 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4921 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5461 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5581 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5641 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     5701 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5761 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     5821 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     5881 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     5941 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6001 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6061 aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     6121 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     6181 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     6241 gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat
     6301 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
     6361 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     6421 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
     6481 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
     6541 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     6601 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     6661 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     6721 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     6781 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
     6841 ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc tcagattgat
     6901 tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg agacccctgc
     6961 ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt
     7021 ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc tgtactagtt
     7081 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg
     7141 ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg
     7201 aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa
     7261 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc
     7321 gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     7381 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca
     7441 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     7501 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     7561 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     7621 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     7681 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     7741 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     7801 tccttctcta ggcgccggaa ttagatctct cgactaggga taacagggta attgtttgaa
     7861 tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt
     7921 acttcttcag gttaacccaa cagaaggctc gaggtgactg gagttcagac gtgtgctctt
     7981 ccgatcgcca ccatgcctaa tcatcagtcc gggtcaccta ccggcagttc agacctgctc
     8041 cttgatggca agaaacaacg agcccatctg gcgctgagga gaaaacggcg acgggaaatg
     8101 cgcaagatta accgaaaggt gagaagaatg aatctcgcac ccattaaaga aaaaacagcc
     8161 tggcagcacc tgcaagctct gatcttcgag gcggaagaag tgttgaagac ttctcaaact
     8221 ccacagacct ccctcacgct gtttctagca ctcttggccg tattggcccc gccacctgtg
     8281 agcgggatga gcatcatctt cttcctgccc ctgtaaggcc aaac
//
