LOCUS       sequence-202326-       15189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in 
            vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15189)
  AUTHORS   Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, 
            Janke C, Schultz RM, Lampson MA
  TITLE     Spindle asymmetry drives non-Mendelian chromosome segregation.
  JOURNAL   Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092.
  PUBMED    29097549
REFERENCE   2  (bases 1 to 15189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "668-672"
COMMENT     Sequence Label: 6x Halo-EGFP- 2x PACT
FEATURES             Location/Qualifiers
     source          1..15189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    312..345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             350..949
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(350..369)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     846..866
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1085..1102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1091..1202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1347..1650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1652..1929
                     /label=chicken beta-actin promoter
     intron          1930..2946
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2870..2892
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2954..2973
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     3040..3059
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3040..3058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3101..3991
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             4019..4906
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     regulatory      4937..4946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4943..5833
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      5840..5849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5864..6754
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             6782..7669
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     regulatory      7700..7709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7706..8596
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      8603..8612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8609..8611,8612..8614,8615..9325)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8609..8611,8612..8614,8615..9325)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8654..8675)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8915..8934)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9262..9283
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10431..11004
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    10454..11006
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10598..10615)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10825..10844
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11006..11713
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11151..11169)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11419..11438
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             11732..12205
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             12206..12235
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(12336..12355)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    12401..12456
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(12455..12474)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    12817..12850
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    12863..12984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12900..12919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12954..12973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13003..13023)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13003..13021)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13042..13058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13042..13058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13055..13077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13066..13082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13090..13096,13097..13114,13115..13120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13135..13156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13273..13290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13444..14032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13524..13543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14203..14994,14995..15063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14826..14845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15064..15168)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgctgc agtacggcgc gatcgaaatt aatacgactc actataggga
     3061 gacccaagct ctagcgctac cggtcgccac ccgcgccaca atggcagaga tcggtactgg
     3121 cttcccattc gatccccatt acgtggaagt cctgggcgaa cgcatgcact atgtcgatgt
     3181 tggtccgcgc gacggcaccc ctgtgctgtt cctgcatggt aacccgacct cctcctatgt
     3241 gtggcgcaac attatcccgc acgttgcacc gacccatcgc tgtattgctc cagatctgat
     3301 cggtatgggc aagtccgaca agccagacct gggttacttc tttgacgatc acgtccgctt
     3361 tatggatgcc tttatcgagg ccctgggtct ggaagaagtc gtcctggtca ttcatgactg
     3421 gggctccgct ctgggttttc actgggccaa acgcaatcca gaacgcgtca aaggtatcgc
     3481 atttatggaa ttcattcgcc ctatcccgac ctgggacgaa tggccagagt ttgcccgcga
     3541 aaccttccag gcctttcgca ccaccgacgt cggccgcaaa ctgatcattg atcagaatgt
     3601 ttttattgag ggtacgctgc cgatgggtgt cgtccgcccg ctgactgagg tcgagatgga
     3661 tcattatcgc gagccgtttc tgaatcctgt tgatcgcgag ccactgtggc gctttccaaa
     3721 cgaactgcca atcgccggtg aaccagcgaa cattgtcgcg ctggtcgaag agtacatgga
     3781 ttggctgcac cagtcccctg tcccgaaact gctgttctgg ggcaccccag gcgttctgat
     3841 tccaccggcc gaagccgctc gcctggccaa gagcctgcct aactgcaaag ctgtggacat
     3901 tggcccgggt ctgaatctgc tgcaagagga caatccggac ctgattggca gcgagattgc
     3961 gcgctggctg tcgacgctcg agatctccgg cgcttcgaat tctgcagtcg acggtaccgc
     4021 agaaattggt actggctttc catttgaccc ccactatgtg gaggtcctgg gcgagcgcat
     4081 gcattacgtc gacgttggtc cgcgcgatgg cacccctgtg ctgtttctgc acggtaatcc
     4141 gacctcctcc tacgtgtggc gcaatatcat tccgcatgtt gcaccgaccc accgctgcat
     4201 cgctccagac ctgattggta tgggcaaatc cgataaacca gatctgggtt attttttcga
     4261 tgaccatgtc cgcttcatgg acgccttcat tgaagccctg ggtctggagg aggtcgtcct
     4321 ggtcatccac gattggggct ccgctctggg tttccattgg gccaagcgca acccagagcg
     4381 cgtcaagggt attgcattca tggagtttat ccgccctatt ccgacctggg acgagtggcc
     4441 agaattcgcc cgcgagacct ttcaggcctt ccgcaccacc gatgtcggcc gcaagctgat
     4501 tatcgaccag aacgttttca tcgaaggtac gctgccgatg ggtgtcgtcc gcccgctgac
     4561 tgaagtcgaa atggaccact accgcgaacc gttcctgaac cctgttgacc gcgaaccact
     4621 gtggcgcttc ccaaatgagc tgccaattgc cggtgagcca gcgaatatcg tcgcgctggt
     4681 cgaggaatat atggactggc tgcatcagtc ccctgtcccg aagctgctgt tttggggcac
     4741 cccaggcgtt ctgatcccac cggccgaggc cgctcgcctg gccaaaagcc tgcctaattg
     4801 caaggctgtg gatatcggcc cgggtctgaa cctgctgcaa gaagataacc cggatctgat
     4861 cggcagcgaa atcgcgcgct ggctgtcgac gctcgaaatt tccggcagtg ctggtagtgc
     4921 tggtgaattg atgcgcgcca ccatggcaga aatcggtact ggctttccat tcgaccccca
     4981 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
     5041 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
     5101 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
     5161 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
     5221 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
     5281 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
     5341 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
     5401 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
     5461 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
     5521 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
     5581 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
     5641 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
     5701 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     5761 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct
     5821 cgagatttcc ggttacgtag ccaccatggt ggggcgcgcc acaatggcag agatcggtac
     5881 tggcttccca ttcgatcccc attacgtgga agtcctgggc gaacgcatgc actatgtcga
     5941 tgttggtccg cgcgacggca cccctgtgct gttcctgcat ggtaacccga cctcctccta
     6001 tgtgtggcgc aacattatcc cgcacgttgc accgacccat cgctgtattg ctccagatct
     6061 gatcggtatg ggcaagtccg acaagccaga cctgggttac ttctttgacg atcacgtccg
     6121 ctttatggat gcctttatcg aggccctggg tctggaagaa gtcgtcctgg tcattcatga
     6181 ctggggctcc gctctgggtt ttcactgggc caaacgcaat ccagaacgcg tcaaaggtat
     6241 cgcatttatg gaattcattc gccctatccc gacctgggac gaatggccag agtttgcccg
     6301 cgaaaccttc caggcctttc gcaccaccga cgtcggccgc aaactgatca ttgatcagaa
     6361 tgtttttatt gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aggtcgagat
     6421 ggatcattat cgcgagccgt ttctgaatcc tgttgatcgc gagccactgt ggcgctttcc
     6481 aaacgaactg ccaatcgccg gtgaaccagc gaacattgtc gcgctggtcg aagagtacat
     6541 ggattggctg caccagtccc ctgtcccgaa actgctgttc tggggcaccc caggcgttct
     6601 gattccaccg gccgaagccg ctcgcctggc caagagcctg cctaactgca aagctgtgga
     6661 cattggcccg ggtctgaatc tgctgcaaga ggacaatccg gacctgattg gcagcgagat
     6721 tgcgcgctgg ctgtcgacgc tcgagatctc cggcgcttcg aattctgcag tcgacggtac
     6781 cgcagaaatt ggtactggct ttccatttga cccccactat gtggaggtcc tgggcgagcg
     6841 catgcattac gtcgacgttg gtccgcgcga tggcacccct gtgctgtttc tgcacggtaa
     6901 tccgacctcc tcctacgtgt ggcgcaatat cattccgcat gttgcaccga cccaccgctg
     6961 catcgctcca gacctgattg gtatgggcaa atccgataaa ccagatctgg gttatttttt
     7021 cgatgaccat gtccgcttca tggacgcctt cattgaagcc ctgggtctgg aggaggtcgt
     7081 cctggtcatc cacgattggg gctccgctct gggtttccat tgggccaagc gcaacccaga
     7141 gcgcgtcaag ggtattgcat tcatggagtt tatccgccct attccgacct gggacgagtg
     7201 gccagaattc gcccgcgaga cctttcaggc cttccgcacc accgatgtcg gccgcaagct
     7261 gattatcgac cagaacgttt tcatcgaagg tacgctgccg atgggtgtcg tccgcccgct
     7321 gactgaagtc gaaatggacc actaccgcga accgttcctg aaccctgttg accgcgaacc
     7381 actgtggcgc ttcccaaatg agctgccaat tgccggtgag ccagcgaata tcgtcgcgct
     7441 ggtcgaggaa tatatggact ggctgcatca gtcccctgtc ccgaagctgc tgttttgggg
     7501 caccccaggc gttctgatcc caccggccga ggccgctcgc ctggccaaaa gcctgcctaa
     7561 ttgcaaggct gtggatatcg gcccgggtct gaacctgctg caagaagata acccggatct
     7621 gatcggcagc gaaatcgcgc gctggctgtc gacgctcgaa atttccggca gtgctggtag
     7681 tgctggtgaa ttgatgcgcg ccaccatggc agaaatcggt actggctttc cattcgaccc
     7741 ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg
     7801 cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc gcaacatcat
     7861 cccgcatgtt gcaccgaccc atcgctgcat tgctccagac ctgatcggta tgggcaaatc
     7921 cgacaaacca gacctgggtt atttcttcga cgaccacgtc cgcttcatgg atgccttcat
     7981 cgaagccctg ggtctggaag aggtcgtcct ggtcattcac gactggggct ccgctctggg
     8041 tttccactgg gccaagcgca atccagagcg cgtcaaaggt attgcattta tggagttcat
     8101 ccgccctatc ccgacctggg acgaatggcc agaatttgcc cgcgagacct tccaggcctt
     8161 ccgcaccacc gacgtcggcc gcaagctgat catcgatcag aacgttttta tcgagggtac
     8221 gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt accgcgagcc
     8281 gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc
     8341 cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc tgcaccagtc
     8401 ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac cggccgaagc
     8461 cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc cgggtctgaa
     8521 tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct ggctgtcgac
     8581 gctcgagatt tccggttacg tagccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     8641 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     8701 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     8761 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     8821 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     8881 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     8941 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     9001 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     9061 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     9121 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     9181 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     9241 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     9301 tctcggcatg gacgagctgt acaagggtgg tgaatccggt gatgcatcgt atcgattgct
     9361 agccaacatt gaagccatca ttgcctctga aaaagaagta tggaacagag aaaaattgac
     9421 tctccagaaa tctttgaaaa gggcagaggc tgaagtatac aaactgaaag ctgaactaag
     9481 aaatgactct ttacttcaaa ctctgagccc tgattctgaa catgtcactt taaagagaat
     9541 ttatggtaaa tacttgaggg cagaaagttt tcgaaaggct ctcatttacc agaagaaata
     9601 cctgctgctg ttactgggtg ggttccagga atgtgaagat gccaccttgg ccctgcttgc
     9661 ccggatgggg gggcagccag ctttcacgga tctagaggtg atcaccaatc gcccaaaggg
     9721 cttcaccagg tttcggtcgg ccgtcagagt atccattgca atttccagaa tgaaattttt
     9781 ggttcgacgg tggcatcgag tcacaggttc tgtttccatc aatattaaca gagatggctt
     9841 tggactgaat caaggtgcac tgtacaaggg tggtgaatcc ggtgatgcat cgtatcgatt
     9901 gctagccaac attgaagcca tcattgcctc tgaaaaagaa gtatggaaca gagaaaaatt
     9961 gactctccag aaatctttga aaagggcaga ggctgaagta tacaaactga aagctgaact
    10021 aagaaatgac tctttacttc aaactctgag ccctgattct gaacatgtca ctttaaagag
    10081 aatttatggt aaatacttga gggcagaaag ttttcgaaag gctctcattt accagaagaa
    10141 atacctgctg ctgttactgg gtgggttcca ggaatgtgaa gatgccacct tggccctgct
    10201 tgcccggatg ggggggcagc cagctttcac ggatctagag gtgatcacca atcgcccaaa
    10261 gggcttcacc aggtttcggt cggccgtcag agtatccatt gcaatttcca gaatgaaatt
    10321 tttggttcga cggtggcatc gagtcacagg ttctgtttcc atcaatatta acagagatgg
    10381 ctttggactg aatcaaggtg catccggatg acgataagct taaggatccg cccctctccc
    10441 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
    10501 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
    10561 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
    10621 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
    10681 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
    10741 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
    10801 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
    10861 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt
    10921 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
    10981 ttcctttgaa aaacacgatg ataatatggt gagcaagggc gaggaggata acatggccat
    11041 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
    11101 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
    11161 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
    11221 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
    11281 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
    11341 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
    11401 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
    11461 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
    11521 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
    11581 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
    11641 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
    11701 cgagctgtat aaggccggcc tggagtctag aatcagtctg attgcggcgt tagcggtaga
    11761 tcgcgttatc ggcatggaaa acgccatgcc gtggaacctg cctgccgatc tcgcctggtt
    11821 taaacgcaac accttaaata aacccgtgat tatgggccgc catacctggg aatcaatcgg
    11881 tcgtccgttg ccaggacgca aaaatattat cctcagcagt caaccgggta cggacgatcg
    11941 cgtaacgtgg gtgaagtcgg tggatgaagc catcgcggcg tgtggtgacg taccagaaat
    12001 catggtgatt ggcggcggtc gcgtttatga acagttcttg ccaaaagcgc aaaaactgta
    12061 tctgacgcat atcgacgcag aagtggaagg cgacacccat ttcccggatt acgagccgga
    12121 tgactgggaa tcggtattca gcgaattcca cgatgctgat gcgcagaact ctcacagcta
    12181 ttgctttgag attctggagc ggcgggaaca aaaactcatc tcagaagagg atctttaatt
    12241 aagcgctcga ggcggccgcc tcctcaggtg caggctgcct atcagaaggt ggtggctggt
    12301 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
    12361 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
    12421 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
    12481 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgccatatgc
    12541 tggctgccat gaacaaaggt ggctataaag aggtcatcag tatatgaaac agccccctgc
    12601 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
    12661 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
    12721 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc
    12781 cctcgacctg agcgtcgagc tagcatatgg atccatataa cttcgtataa tgtatgctat
    12841 acgaagttat tacgataccg tcaacttgtt tattgcagct tataatggtt acaaataaag
    12901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12961 gtccaaactc atcaatgtat cttaggtacc cagcttttgt tccctttagt gagggttaat
    13021 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    13081 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    13141 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    13201 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    13261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    13321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    13381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    13441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    13501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    13561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    13621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    13681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    13741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    13921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    13981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    14041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    14101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    14161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    14221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    14281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    14341 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    14401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    14461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    14521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    14581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    14641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    14701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    14761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    14821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    14881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    14941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    15001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    15061 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    15121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    15181 aaaagtgcc
//
