LOCUS       sequence-202406-        8765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: p3-HPRT1-S104Rf-PdTK-mCh
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1026..1079
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1089..1685
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1089..1108)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1585..1605
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1692..2687
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(2719..2736)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2725..2949
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2968..3347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3349..3626
                     /label=chicken beta-actin promoter
     intron          3627..4642
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4566..4588
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4650..4669
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4736..4760
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4766..5476
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4911..4929)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5179..5198
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5373..5396
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5478..5502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5598..5617)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5663..5718
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5717..5736)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     6490..6507
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6491..6507
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6528..6551)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     primer_bind     complement(6631..6648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6802..7390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6882..6901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7561..8352,8353..8421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8184..8203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8594..8612
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8650..8672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
       61 acagatgcgt aaggagcgat cgcgaaaata ccgcatcagg cggtgcagtg cagcagaatg
      121 atcacagttc actgcagtct caacctcctg ggttcaagcg atccttccac ctcagcctcc
      181 tgagtagctg ggactatagg tgtgcgccac cactcccagc taatattttt attttgtaga
      241 aacagggttc gccatgttac ccagcctccc aaagtgctgg gattacaggc atgagccact
      301 ggcccagttt ctacagtctc tcttaatatt gtatattatc caagaaattt catttaatca
      361 gaacctgcca gtctgatagg tgaaaatggt atcttgtttt tatttgcatt taaaaaaaat
      421 tatgatagtg gtatgcttgg tttttttgaa ggtatcaaat tttttacctt atgaaacatg
      481 agggcaaagg atgtgttacg tggaagattt aaaaaaaatt tttaatgcat ttttttgaga
      541 caaggtcttg ctctattgtc caggctggag tgcagtggca caatcacagt tcactccagc
      601 ctcaacatcc tgcactaaag tgattttccc acctcacctc tcaagtagct gggactacag
      661 gtacatgcta ccatgcctgg ctaatttttt tttttttgca ggcatggggt ctcactatat
      721 tgcccaggtt ggtgtggaag tttaatgact aagaggtgtt tgttataaag tttaatgtat
      781 gaaactttct attaaattcc tgattttatt tctgtaggac tgaacgtctt gctcgagatg
      841 tgatgaagga gatgggaggc catcacattg tagccctctg tgtgctcaag gggggctata
      901 aattctttgc tgacctgctg gattacatca aagcactgaa tagaaatagt gatagatcca
      961 ttcctatgac tgtagatttt atcagactta agagatattg taccaccggc aagctgcccg
     1021 tgcccgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     1081 ggctcgagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     1141 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     1201 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     1261 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     1321 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     1381 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     1441 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     1501 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     1561 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     1621 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     1681 gtgccggatc catgcccacg ctactgcggg tttatataga cggtccccac gggatgggga
     1741 aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac
     1801 ccgagccgat gacttactgg cgggtgctgg gggcttccga gacaatcgcg aacatctaca
     1861 ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga
     1921 caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc
     1981 atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg
     2041 accgccatcc catcgccgcc ctcctgtgct acccggccgc gcggtacctt atgggcagca
     2101 tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacca
     2161 acatcgtgct tggggccctt ccggaggaca gacacatcga ccgcctggcc aaacgccagc
     2221 gccccggcga gcggctggac ctggctatgc tggctgcgat tcgccgcgtt tacgggctac
     2281 ttgccaatac ggtgcggtat ctgcagtgcg gcgggtcgtg gcgggaggac tggggacagc
     2341 tttcggggac ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac
     2401 cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg
     2461 gcgacctgta taacgtgttt gcctgggcct tggacgtctt ggccaaacgc ctccgttcca
     2521 tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc
     2581 aacttacctc cgggatggtc cagacccacg tcaccacccc cggctccata ccgacgatat
     2641 gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgatct agagggcccg
     2701 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2761 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2821 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     2881 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     2941 gctctatggg ctagcggtgg cggcctcgac attgattatt gactagttat taatagtaat
     3001 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3061 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
     3361 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     3421 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag
     3481 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     3541 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     3601 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
     3661 gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     3721 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     3781 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     3841 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     3901 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     3961 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     4021 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     4081 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     4141 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     4201 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     4261 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     4321 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     4381 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     4441 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     4501 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     4561 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     4621 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     4681 ttttggcaaa gaattcgcgg ccgcggtacc ggcgcgccgg atcctgcagg ttatcacaag
     4741 tttgtacaaa aaagcaggct ccaccatggt gagcaagggc gaggaggaca acatggccat
     4801 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     4861 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4921 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     4981 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     5041 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     5101 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     5161 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     5221 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     5281 gaaggacggc ggccactacg acgccgaggt caagaccacc tacaaggcca agaagcccgt
     5341 gcagctgccc ggcgcctaca acgtcaacat caagctggac atcacctccc acaacgagga
     5401 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcggcatgga
     5461 cgagctgtac aagtaagacc cagctttctt gtacaaagtg gtgataactc tagagaattc
     5521 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     5581 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     5641 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     5701 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     5761 aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc atgaacaaag
     5821 gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     5881 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     5941 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6001 actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc tgcagcccaa
     6061 gcttggatcc ctcgagttaa ttaaatctag aagtcgacgg gcacgggcag cttgccggtg
     6121 gtaagagata ttgtgtgagt atatttaata tatgattctt tttagtggca acagtaggtt
     6181 ttcttatatt ttctttgaat ctctgcaaac catacttgct ttcatttcac ttggttacag
     6241 tgagattttt ctaacatatt cactagtact ttacatcaaa gccaatactg tttttttaaa
     6301 actagtcacc ttggaggata tatacttatt ttacaggtgt gtgtggtttt ttaaataaac
     6361 tccttttagg aattgctgtt gggacttggg atactttttt cactatacat actggtgaca
     6421 gataccctct cttgagctac atcggtttgt ggggagtcaa aagtcctttg gagctaggtt
     6481 tgacaaattt gtaaaacgac ggccagtgcg gccgcgaatt cggtacccta actaacagga
     6541 agagtttgta ggggcccccc ctcgactgca ttaatgaatc ggccaacgcg cggggagagg
     6601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8581 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8641 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8701 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     8761 gctta
//
