LOCUS       sequence-202523-        9770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses huAsCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY026
FEATURES             Location/Qualifiers
     source          1..9770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        369..748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        749..952
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     903..923
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     997..1016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        997..1015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1063..4983
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4703..4712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4984..5031
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5038..5118
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5192..5209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5198..5422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5468..5896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5555..5574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5765..5786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5905..5925)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5910..6239
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6090..6225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6152..6171
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6306..7100
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6360..6379)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6970..6989
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7274..7395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7311..7330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7365..7384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7444..7460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7444..7460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7457..7479)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7468..7484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7492..7498,7499..7516,7517..7522))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7537..7558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7675..7692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7846..8434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7926..7945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8605..9396,9397..9465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9228..9247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9466..9570)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9645..9664)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg taatttctac tcttgtagat ggagacgata tatcgtctct ttttcaattg
      301 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      361 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      421 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      481 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      541 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      601 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      661 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      721 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      781 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      841 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      901 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
      961 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     1021 ccaagctggc tagcgtttaa acttaagctt ggtaccgcca ccatgacaca gttcgagggc
     1081 tttaccaacc tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag
     1141 accctgaagc acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac
     1201 tacaaggagc tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg
     1261 cagctggtgc agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag
     1321 aaaaccgagg agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc
     1381 cacgactact tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc
     1441 gagatctaca agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg
     1501 ggcaccgtga ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca
     1561 acctacttct ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc
     1621 acagccatcc cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac
     1681 atcttcacac gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag
     1741 aaggccatcg gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat
     1801 aaccagctgc tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct
     1861 cgggaggcag gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag
     1921 aagaatgatg agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt
     1981 aagcagatcc tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac
     2041 gaggaagtga tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg
     2101 gagacagccg aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc
     2161 agccacaaga agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg
     2221 aatgccctgt atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag
     2281 gagaaggtgc agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc
     2341 gcaggcaagg agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca
     2401 cacgccgccc tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc
     2461 ctgaagtctc agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg
     2521 gatgagtcca acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag
     2581 atggagcctt ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac
     2641 tccgtggaga agttcaagct gaactttcag atgcctacac tggcctctgg ctgggacgtg
     2701 aataaggaga agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc
     2761 atcatgccaa agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc
     2821 agcgagggct ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca
     2881 aagtgcagca cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc
     2941 ctgctgtcca acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac
     3001 aatcctgaga aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag
     3061 aagggctaca gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag
     3121 tataccaaga caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac
     3181 ctgggcgagt actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc
     3241 gccgagaagg agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat
     3301 aacaaggact ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc
     3361 ggcctgtttt ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag
     3421 ctgttctacc gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg
     3481 ctgaacaaga agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg
     3541 tacgactatg tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg
     3601 cccaacgtga tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc
     3661 gacaagttct ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct
     3721 aagttcaacc agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc
     3781 atcgatcggg gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc
     3841 ctggagcagc ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac
     3901 agggagaagg agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat
     3961 ctgaagcagg gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac
     4021 caggccgtgg tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc
     4081 gccgagaagg ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg
     4141 gtgctgaagg actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca
     4201 gaccagttca cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct
     4261 gccccatata catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa
     4321 accatcaaga atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac
     4381 gacgtgaaaa ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag
     4441 aggggcctgc ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag
     4501 tttgacgcca agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat
     4561 cacagattca ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg
     4621 gaggagaagg gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat
     4681 gacgattctc acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg
     4741 aactccaatg ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc
     4801 gtgtgcttcg actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc
     4861 gcctaccaca tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat
     4921 ctgaagctgc agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc
     4981 aacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac
     5041 ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat
     5101 gatgtccccg actatgccta agaattctgc agatatccag cacagtggcg gccgctcgag
     5161 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5221 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5281 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5341 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5401 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     5461 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5521 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5581 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5641 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5701 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5761 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5821 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5881 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6001 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6061 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6121 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     6181 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6241 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     6301 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6361 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6421 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6481 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6541 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6601 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6661 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6721 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6781 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     6841 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6901 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6961 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7021 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7081 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     7141 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7201 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7261 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7321 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7381 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7441 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7501 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7561 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7621 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7681 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7741 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8281 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8521 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8581 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8641 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8701 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8761 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8821 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8881 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8941 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9001 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9061 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9121 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9181 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9241 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9301 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9361 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9421 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9481 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9541 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9601 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9661 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     9721 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acttaattaa
//
