LOCUS       sequence-202535-       10370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Sec7 with 
            Sec7-mCherry2Bx6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Day KJ, Casler JC, Glick BS
  TITLE     Budding Yeast Has a Minimal Endomembrane System.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 
            10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.
  PUBMED    29316441
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.12.014"; journalName: "Dev Cell"; date: 
            "2018-01-8- 8"; volume: "44"; issue: "1"; pages: "56-72.e4"
COMMENT     Sequence Label: YIplac211-SEC7-mCherry2Bx6
FEATURES             Location/Qualifiers
     source          1..10370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7028..7044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7028..7044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7041..7063)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7052..7068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7076..7082,7083..7100,7101..7106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7121..7142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7259..7276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7430..8018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7510..7529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8189..8980,8981..9049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8812..8831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9050..9154)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9284..10084)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQLN"
     promoter        complement(10085..10300)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     10343..10361
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctattgttgt caaatgtgtt
      421 ttacagcttt tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac
      481 tgtatccctt acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt
      541 tctcgtgact ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt
      601 gataaaatac caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt
      661 atgtttcagt tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc
      721 accatttgca tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt
      781 agtatcaatg cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat
      841 gatgaagact ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat
      901 aagagcgttc caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa
      961 ttataccttt ctacggatcc aggaagttcg gggtccagcg gttcttcagg cgctagcatg
     1021 gatagcactg agagcggctc caccgagtcc gtcatcaagg agttcatgcg cttcaaggtg
     1081 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1141 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1201 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     1261 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1321 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     1381 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1441 cagaagaaga ctatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     1501 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     1561 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     1621 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1681 gccgagggcc gccactccgg ctcccaggga tctagcggca gcagttctag catggatagc
     1741 actgagagcg gctccaccga gtccgtcatc aaggagttca tgcgcttcaa ggtgcacatg
     1801 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     1861 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     1921 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     1981 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2041 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2101 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2161 aagactatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     2221 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     2281 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     2341 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     2401 ggccgccact ccggctccca gggatctagc ggcagcagtt ctagcatgga tagcactgag
     2461 agcggctcca ccgagtccgt catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2521 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2581 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2641 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2701 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2761 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2821 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagact
     2881 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2941 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     3001 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     3061 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     3121 cactccggct cccagggatc tagcggcagc agttctagca tggatagcac tgagagcggc
     3181 tccaccgagt ccgtcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3241 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3301 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3361 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3421 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3481 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3541 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc
     3601 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     3661 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     3721 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     3781 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     3841 ggctcccagg gatctagcgg cagcagttct agcatggata gcactgagag cggctccacc
     3901 gagtccgtca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3961 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     4021 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     4081 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     4141 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     4201 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     4261 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagactat gggctgggag
     4321 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     4381 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     4441 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     4501 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccggctcc
     4561 cagggatcta gcggcagcag ttctagcatg gatagcactg agagcggctc caccgagtcc
     4621 gtcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4681 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4741 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4801 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4861 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4921 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4981 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ctatgggctg ggaggcctcc
     5041 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5101 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     5161 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     5221 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccgg ctcccaggga
     5281 tctagcggca gcagttaagc ggccgggctt tgttgaatgg tccagcagat taaaatatgc
     5341 ttagttgtag aacacagaaa aaatgagaag gttgatatac ttttactcat atttaccctg
     5401 atttaatggc ggtgatcctt tttagattcg tttaattcat aaacaaggta catcatatag
     5461 caataacaaa tatgtaaact atgtagaaag acttatcaag tttagccatc ttggtcctca
     5521 ttcatgggca ttttatacta acttcatttt tgccgaaata agaaaggatg taatcttaac
     5581 ctaaaatatg taagcaatta agggtgcacg tagttacttg cgaacaaata ggaaaagaaa
     5641 gaaacaagca cggatactga atgactgagt gtattactac acgatttatc tcaacgaatt
     5701 taagtgtgga ggacgatcgg ctgcacgtag gagtcaacgt ttttttactg tgttcatcac
     5761 aaaaccatta agctgagtca tccatattgc aaacaataag gtagcaccgc ttccatcaaa
     5821 acactagaac ataccgatgt tttcacggat ttattggaaa caaatagcag atgagggcgt
     5881 ttttacttgt aatgaaggcg aatgagaagg caaggcttac aggcttgttg tttatgccaa
     5941 gcatcaatac tcgctatgga cgagcgccat cagcgataac aacaaagttt gcaagtgctt
     6001 gcttggatat aaaaggcttg acgcttaaga taaatagggc catacttgct tgcttttact
     6061 atttttgttc ctcataagcc tgattgacta cgctattata aacccctaca ttatcctata
     6121 atcaacttca cttgtgtaac gttgaatgag ttcaactgag taatactcgg tcggaaatat
     6181 acgctccatc tggcaggaaa cataggctgc atttatatct tgcacaatta tgcccttcgt
     6241 aggtgtatgt tttcatgttt tccattatgt caatgtagat gtatctttgg acaggcaact
     6301 tttggatcaa taaagcacaa tttttgagca atttgagata tccagtattt gtacgggagt
     6361 cccaagattg tttagcctaa aaggttgagg acttggatta ttcttccatc aagcgcctcc
     6421 acccctcgca tcctatttct tttgaagcat aggggacgag actgtctgta atttatggcg
     6481 aagtatgaca agaaagattc aaactttgtt ttacgtatgc cccaataaat tctatggtag
     6541 cactactaga gacgtatttt ggttttgtaa taattttcaa actgctcgct atgatttgtt
     6601 acaagaaaca aacaaaagac acgaccatag ggttcatgtt ggtggttgcg aagccacatc
     6661 tttaaaacat tactgcaaat tcagcaggag tgagtagcaa aatcgacaaa aactagagta
     6721 cgcaacattc ccggtcgtaa atttcttatc gtggcgccag aacattcccg tccaatgatg
     6781 agaatgtctc taaaacaaat gcagtcacag agattttctg cgacttctcg agtgagaatg
     6841 ttgcttattg gtgctagtgg cttgagatct tcgtcaagca aattggaatg cccccagatt
     6901 ctcaaacaag cacggcagaa actgaaatac ggccaaaatt atgaaccctt ttgtgggatc
     6961 tttgtagaaa taggacctca ggatagtatg atgaagaatg ttggcatgca agcttggcgt
     7021 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7081 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7141 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7201 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7861 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     9181 gctttttctt tccaattttt tttttttcgt cattataaaa atcattacga ccgagattcc
     9241 cgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagc tggccgcatc
     9301 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
     9361 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
     9421 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
     9481 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
     9541 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
     9601 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
     9661 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
     9721 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt
     9781 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg
     9841 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca
     9901 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct
     9961 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa
    10021 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg
    10081 acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg
    10141 ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct
    10201 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc
    10261 gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aagctctaag aaaccattat
    10321 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
