LOCUS       sequence-20256-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, 
            Dauter Z, Tuschl T, Patel DJ
  TITLE     Crystal structure of A. aeolicus argonaute, a site-specific 
            DNA-guided endoribonuclease, provides insights into RISC-mediated 
            mRNA cleavage.
  JOURNAL   Mol Cell. 2005 Aug 5. 19(3):405-19.
  PUBMED    16061186
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2005 Aug 5. 19(3):405-19."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago Chi-d
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             70..96
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatccatccg atagtggtgg tggaattctg atcgccgccg ccatggacta caaggacgac
       61 gatgacaagt acccttatga cgtgcccgat tacgctttga accagatatc aacaagtttg
      121 tacaaaaaag caggctccac catgggaacc aattcagtcg actggatccg gatgtactcg
      181 ggagccggcc ccgcacttgc acctcctgcg ccgccgcccc ccatccaagg atatgccttc
      241 aagcctccac ctagacccga ctttgggacc tccgggagaa caatcaaatt acaggccaat
      301 ttcttcgaaa tggacatccc caaaattgac atctatcatt atgaattgga tatcaagcca
      361 gagaagtgcc cgaggagagt taacagggaa atcgtggaac acatggtcca gcactttaaa
      421 acacagatct ttggggatcg gaagcccgtg tttgacggca ggaagaatct atacacagcc
      481 atgccccttc cgattgggag ggacaaggtg gagctggagg tcacgctgcc aggagaaggc
      541 aaggatcgca tcttcaaggt gtccatcaag tgggtgtcct gcgtgagctt gcaggcgtta
      601 cacgatgcac tttcagggcg gctgcccagc gtcccttttg agacgatcca ggccctggac
      661 gtggtcatga ggcacttgcc atccatgagg tacacccccg
//
