LOCUS       sequence-202628-        1830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for potassium ion ratiometric indicator
            KIRIN1-GR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Shen Y, Wu SY, Rancic V, Aggarwal A, Qian Y, Miyashita SI, Ballanyi 
            K, Campbell RE, Dong M
  TITLE     Genetically encoded fluorescent indicators for imaging intracellular
            potassium ion concentration.
  JOURNAL   Commun Biol. 2019 Jan 14;2:18. doi: 10.1038/s42003-018-0269-2. 
            eCollection 2019.
  PUBMED    30652129
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-01-14"; volume: "2:18. doi"; pages: " 
            10.1038/s42003-018-0269-2. eCollection 2019"
COMMENT     Sequence Label: pBAD-KIRIN1-GR
FEATURES             Location/Qualifiers
     source          1..1830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
      301 ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacttcaa cagccacaac gtctatatca cggccgacaa gcagaagaac
      481 ggcatcaagg ctaacttcaa gatccgccac aacgttgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagcc atcagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgtcgac ggcctgttca acttcgtgaa ggacgccggc
      721 gagaagctgt gggatgccgt gacaggccag cacgacaagg acgaccaggc caagaaggtg
      781 caggaacacc tgaacaagac cggcatcccc gacgccgaca aagtgaacat ccagatcgcc
      841 gacggcaagg ccacagtgac aggcgacgga ctgagccagg aagccaaaga aaagatcctg
      901 gtggccgtgg gcaacatcag cggaatcgcc agcgtggacg atcaagtgaa aaccgccacc
      961 cctgccaccg ccagccagtt ctacacagtg aagtccggcg acaccctgag cgccatcagc
     1021 aaacaggtgt acggcaacgc caacctgtac aacaagattt tcgaggccaa caagcccatg
     1081 ctgaagtccc ccgacaaaat ctaccccggc caggtgctga gaatccccga ggaactggaa
     1141 ttcatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
     1201 ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg gaactcaaac catgaggatc
     1261 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
     1321 tatggcagcc gtacttttat caagtacccg aaaggcattc ctgatttctt taaacagtcc
     1381 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
     1441 gtcatgcagg acaccagcct tgaggatggc tgtctcgttt accacgtcca agtcagaggg
     1501 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
     1561 acagagatga tgtatccagc agatggtggt ctgaggggat acactcatat ggcactgaaa
     1621 gttgatggtg gtggccatct gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc
     1681 gtcgggaaca tcaagatgcc cggtatccat gccgttgatc accgcctgga aaggttagag
     1741 gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg
     1801 cttggtggtg ggatggacga gctgtacaag
//
