LOCUS       sequence-20263-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  TITLE     Rnl2 K227Q mutant
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET16b-Rnl2(1-249)K227Q
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..64
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             77..88
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg gggccatcat catcatcatc atcatcatca
       61 tcacagcagc ggccatatcg aaggtcgtca tatgtttaaa aagtatagca gtcttgaaaa
      121 tcattacaac tctaaattta ttgaaaaact ttatagcttg ggattgactg gtggggagtg
      181 ggtagctcgt gaaaagattc acggcacaaa tttctcattg attattgagc gtgataaagt
      241 gacttgcgct aaacgcactg gaccgattct tcctgctgaa gatttctttg ggtatgaaat
      301 tattttgaag aattatgctg attccattaa agctgtacaa gatattatgg aaacctcagc
      361 ggttgtatct tatcaagtct ttggcgaatt cgctggacct ggcattcaga agaatgttga
      421 ttatggtgat aaagattttt atgtatttga cattattgtt actacagaaa gcggtgatgt
      481 gacttatgta gatgattata tgatggaatc attctgtaat acatttaaat ttaaaatggc
      541 tccactttta ggtcgcggta aatttgaaga gcttattaaa ttgccaaatg atttagattc
      601 tgtcgtccaa gattataatt ttacagtaga ccatgctgga ttagttgatg caaataaatg
      661 cgtttggaa
//
