LOCUS       sequence-202638-       14132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSC218UG-At Ubiquitin10:Gateway  expression cassette binary vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Curtin SJ, Xiong Y, Michno JM, Campbell BW, Stec AO, Cermak T, 
            Starker C, Voytas DF, Eamens AL, Stupar RM
  TITLE     CRISPR/Cas9 and TALENs generate heritable mutations for genes 
            involved in small RNA processing of Glycine max and Medicago 
            truncatula.
  JOURNAL   Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
  PUBMED    29087011
REFERENCE   2  (bases 1 to 14132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857."
COMMENT     Sequence Label: pSC6
FEATURES             Location/Qualifiers
     source          1..14132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(122..493)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            526..635
                     /note="incP origin of transfer"
     primer_bind     858..877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1111..1128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1316..1966)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2431..2455
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     2721..2737
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2721..2737
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(2755..3007)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    3071..3195
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3236..3541)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3338..3357)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3861..4541)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     4456..4475
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(4595..4601,4602..4619,4620..4625))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(4650..4774)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5457..5473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6009..6354
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6297..6317
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6375..6926
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      7070..7322
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7335..7351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7491..7510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7701..7722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7969..7993
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9589..10737)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11036..11830)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12022..12789
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      13482..14117
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct tcttgatgga
       61 gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa
      121 gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct
      181 tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg
      241 ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc
      301 gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga
      361 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc
      421 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg
      481 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
      541 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      601 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      661 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      721 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag cgccacgctt
      781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1141 cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg cttggacgct agggcagggc
     1201 atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat
     1261 gttgtctaca tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat
     1321 cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat
     1381 cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt
     1441 ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc
     1501 cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg
     1561 tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct
     1621 gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc
     1681 catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc
     1741 gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg
     1801 ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa
     1861 cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca
     1921 ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat
     1981 cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc
     2041 ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacatacccc tgatcgtaat
     2101 tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg
     2161 cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc
     2221 gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt
     2281 cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc agatctgggg aaccctgtgg
     2341 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     2401 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     2461 tgatagttta aactgaaggc gggaaacgac aatctgatca tgagcggaga attaagggag
     2521 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga
     2581 accgcaacgt tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca
     2641 tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagacgg
     2701 acgcggccgc tccgactagg caggaaacag ctatgaccat gattacgaat tcccgatcta
     2761 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     2821 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     2881 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     2941 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     3001 aacgatcggg gaaattcgag ctccaccgcg gtggcggccg ctctagaact agttaattaa
     3061 gaattatcga accactttgt acaagaaagc tgaacgagaa acgtaaaatg atataaatat
     3121 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     3181 tatccagtca ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata
     3241 ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag
     3301 ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc
     3361 agctttcatc cccgatatgc accaccgggt aaagttcacg ggggacttta tctgacagca
     3421 gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct
     3481 gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca
     3541 tttcaccagc ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc
     3601 tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca
     3661 ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact
     3721 gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga
     3781 catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata
     3841 cgcatactgt tatctggctt ttagtaagcc ggatcctcta gattacgccc cgcctgccac
     3901 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     3961 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     4021 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     4081 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     4141 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     4201 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     4261 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     4321 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     4381 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     4441 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     4501 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     4561 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt
     4621 gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt
     4681 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     4741 acgtttctcg ttcagctttt ttgtacaaac ttgtttgata gcttggcgcg cctcgagggg
     4801 gggcccggta ccctgttaat cagaaaaact cagattaatc tacaaattcg atcgcacaaa
     4861 ctagaaacta acaccagatc tagatagaaa tcacaaatcg aagagtaatt attcgacaaa
     4921 actcaaatta tttgaacaaa tcggatgata tctatgaaac cctaatcgag aattaagatg
     4981 atatctaacg atcaaaccca gaaaatcgtc ttcgatctaa gattaacaga atctaaacca
     5041 aagaacatat acgaaattgg gatcgaacga aaacaaaatc gaagattttg agagaataag
     5101 gaacacagaa atttaccttg atcacggtag agagaattga gagaaagttt ttaagatttt
     5161 gagaaattga aatctgaatt gtgaagaaga agagctcttt gggtattgtt ttatagaaga
     5221 agaagaagaa aagacgagga cgactaggtc acgagaaagc taaggcggtg aagcaatagc
     5281 taataataaa atgacacgtg tattgagcgt tgtttacacg caaagttgtt tttggctaat
     5341 tgccttattt ttaggttgag gaaaagtatt tgtgctttga gttgataaac acgactcgtg
     5401 tgtgccggct gcaaccactt tgacgccgtt tattactgac tcgtcgaaag cttggcactg
     5461 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg gtattgcccc tgcaggtccc
     5521 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
     5581 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
     5641 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     5701 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     5761 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     5821 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     5881 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     5941 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     6001 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     6061 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     6121 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     6181 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     6241 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     6301 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     6361 actctagagg atccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg
     6421 cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact
     6481 tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc
     6541 gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggcc
     6601 cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc
     6661 gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg
     6721 cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca
     6781 tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg
     6841 ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggta ccgccccgtc
     6901 cggtcctgcc cgtcaccgag atctgaacgg agtgcgcgtg gcatcgcccg agttggagct
     6961 ggtacgggaa ctcatcgaac tcaactggca tacccgcaat ggtgaggtgg aaccgcggcg
     7021 gatcgcgtac gaccgtgccc aggagggggt accgagctcg aatttccccg atcgttcaaa
     7081 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     7141 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     7201 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     7261 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     7321 tcgggaatta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7381 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7441 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc
     7501 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7561 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     7621 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7681 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7741 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag
     7801 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     7861 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta
     7921 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     7981 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     8041 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     8101 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     8161 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     8221 gcctgtgatc aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt
     8281 tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg
     8341 ctggataaag ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga
     8401 tatcaactcc cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac
     8461 tgtcggcctg atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta
     8521 aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac
     8581 ccgctccgat caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga
     8641 ttggcggatc aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc
     8701 aggcggtaaa ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga
     8761 acgccatgga aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt
     8821 ttggtgtcaa caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca
     8881 tcaatcgtat cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca
     8941 cagcgcctac cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc
     9001 tccagaatag cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg
     9061 ttggaatctg tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca
     9121 taccggcgac ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct
     9181 tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt
     9241 cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct
     9301 ttttctcctc gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca
     9361 atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa
     9421 tcacaattgt caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc
     9481 cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag
     9541 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
     9601 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
     9661 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
     9721 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     9781 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     9841 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     9901 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     9961 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
    10021 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
    10081 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
    10141 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
    10201 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
    10261 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
    10321 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
    10381 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
    10441 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
    10501 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
    10561 cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa
    10621 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
    10681 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
    10741 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
    10801 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
    10861 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
    10921 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
    10981 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa
    11041 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
    11101 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
    11161 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
    11221 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    11281 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    11341 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    11401 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    11461 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    11521 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    11581 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    11641 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    11701 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    11761 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    11821 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    11881 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    11941 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    12001 gattttgaaa ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg
    12061 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
    12121 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
    12181 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
    12241 aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt
    12301 tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
    12361 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc
    12421 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt
    12481 gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg
    12541 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
    12601 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
    12661 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
    12721 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
    12781 gtcattaccc aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
    12841 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
    12901 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagatatat
    12961 tatcttatca gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc
    13021 ttcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt
    13081 ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc
    13141 gcacccccga acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa
    13201 attgccggcc ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg
    13261 ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc
    13321 tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc
    13381 ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg
    13441 gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg
    13501 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
    13561 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
    13621 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
    13681 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
    13741 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
    13801 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
    13861 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
    13921 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
    13981 gttttccgcg aggtatccac aacgccggcg gccggccgcg gtgtctcgca cacggcttcg
    14041 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
    14101 ccagcccggt gagcgtcgga aaggcgctcg gt
//
