LOCUS       sequence-20264-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P
  TITLE     GCN5 acetyltransferase complex controls glucose metabolism through 
            transcriptional repression of PGC-1alpha.
  JOURNAL   Cell Metab. 2006 Jun . 3(6):429-38.
  PUBMED    16753578
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2006 Jun . 3(6):429-38."
COMMENT     Sequence Label: pAdEasy Flag GCN5
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(426..449)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(495..698)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(524..544)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcagctggct gctgcagatc catgcggggt gcagtggggg gcttggggtt tttccagcca
       61 ttacacttac aggtttcatt ggccttgcaa gccgagaaga cccctagctt ctcaagcttc
      121 ttggcgcgcg gcagcccccg gacttgcgcc ttcctctgac tggcgcgctg ctgctggctc
      181 aggccaggtc gagccggatc ccccccgctc ccggcccccc cacttcctac cccgggcccc
      241 ccagtccctg tgctgccggc tggggctgca gctggggcag gggctggtgc cggggtggga
      301 gtcggaatcg gggctgaagc cgggctgggt gcaggagtcg gagttggggc aggggctggg
      361 gactgaaggg gccggggctg cgcagccggg gccggggtcg gggcctggga aggttccgcg
      421 aattctttgt catcgtcgtc cttgtagtcc atggtggtac cagatctcta gcggatctga
      481 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      541 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      601 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      661 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      721 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      781 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      841 tgtactgcca agtggg
//
