LOCUS       sequence-202812-        3632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of cell cycle progression by G1/S transition 
            inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Razy-Krajka F, Gravez B, Kaplan N, Racioppi C, Wang W, Christiaen L
  TITLE     An FGF-driven feed-forward circuit patterns the cardiopharyngeal 
            mesoderm in space and time.
  JOURNAL   Elife. 2018 Feb 6;7. pii: 29656. doi: 10.7554/eLife.29656.
  PUBMED    29431097
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-02-6"; volume: "7. pii: 29656. doi"; pages: " 
            10.7554/eLife.29656"
COMMENT     Sequence Label: FoxFTVC+bpFOG_Cdkn1b.a
FEATURES             Location/Qualifiers
     source          1..3632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1358..1492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1395..1414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1449..1468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1528..1547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1529..1547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1634..1651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1805..2393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1885..1904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2564..3355,3356..3424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3187..3206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3425..3529)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3597..3615
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcgcgccgc gttgtatctt tcgttaagtg ctggcgtcga gcagggctaa cctcatttcg
       61 atttgacgat tcgggtaaaa ccgagcgatg gacaggacct ggaaatggga aaggcttact
      121 agaatcaggg tcgaacgaga taagaagatc aggattcgca aattagcact tggctttttt
      181 acgtagaaac ataggatgtc cgctgagccc cttatatata cgtagtacga atcaccggcc
      241 tggcgttgta taagcgagac cagcgtggac gaaaaggtaa ttgtttccga atggatcgcg
      301 ttctctagag gatccggcaa agcttcgtgt attgtaccgg cccattgtca atcatgcaaa
      361 cttgatatta tattgacaag agaagaaggc agtttaaatt aaaactctaa agtagagaga
      421 cattaatctc agctgacaag gcaggtggtc acagtaagtt catttaaata gttggccaac
      481 aatagccttt ccaagaaagt atttttgttc caggtctata caaaaataac acacaagcgg
      541 ccgcaaccat ggcggacaaa aaacccccgg ttcgacagct ccaaaaactc ccaggagtgc
      601 ggtctgcgcg gagaaattta tttggtcgag atgatagaat tgtggacgat ttagaaagag
      661 acgctgaaga gttgaacagt aaagaacaaa aacgattctc ggacacctgg aactttgatg
      721 cagccaatac ccaaccatta gatggcagat ttgaatggga caccggcgtt ccgctgaatc
      781 aacgaactcg actattgcat gttcccccga ttggtaatgc agattcacat aacgatagtg
      841 agcttgtaga agttaacaac tcaacagaat caaaaaatga agagttgcca cctacgggga
      901 attcctcttc tgtgggctca tgtggatctt cggaaaatca aagtaaaaga aggaaactat
      961 cttcagaatc aactgacgac catgtacccg ctacatcgaa gtcgccccga ctgtgacgtc
     1021 atactgtgcc acggaatgaa ttccagctga gcgccggtcg ctaccattac cagttggtct
     1081 ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat
     1141 ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt
     1201 acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat gacatcaacc
     1261 atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat
     1321 tccaaccgct gtttggtctg ctttctgaca aactcggaac ttgtttattg cagcttataa
     1381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgacaaagt
     1501 caaagcggcc atcagatctg ccggtctccc tatagtgagt cgtattaatt tcgataagcc
     1561 aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1981 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     2041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2221 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3541 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3601 ggcgtatcac gaggccctta cgtattaatt aa
//
