LOCUS       sequence-20291-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Paul JI, Schwarzbauer JE, Tamkun JW, Hynes RO
  TITLE     Cell-type-specific fibronectin subunits generated by alternative 
            splicing.
  JOURNAL   J Biol Chem. 1986 Sep 15. 261(26):12258-65.
  PUBMED    3528152
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1986 Sep 15. 261(26):12258-65."
COMMENT     Sequence Label: pEIIIB
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(290..308)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(291..308)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(598..617)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcgaagctcg aattccgatt cacccgtttg ctgtgtcagt gtagtagggg cactctctcc
       61 gccattaatg agagtgataa cgctgatgtc atagtcaatg ccgggttcca gccctgtaac
      121 tgtgtagtat cctactgagg agtccacaaa atcttcaaaa atggggatcc cttctcctgc
      181 cgcaactact gtgattcggt acccgataat ggtggaagag tttagcgggg tccacctcag
      241 gccgatgctt gagtcagtta tatcaacaaa gctccggaat tccgtgtatt ctatagtgtc
      301 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
      361 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
      421 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
      481 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
      541 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      601 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      661 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      721 tgcatgcacc attccttgcg gcggcggtgc t
//
