LOCUS       sequence-202964-        3744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr62
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(909..927)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(910..927)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(945..961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(945..961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(958..980)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    969..985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(993..999,1000..1017,1018..1023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1038..1059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1176..1193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1347..1935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1427..1446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2106..2897,2898..2966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2729..2748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2967..3071)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3149..3604)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3286..3307)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3498..3517
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3730..3744,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3744,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tacccccgcc tccacgtccc
      121 gatgtacttt ttcctcagtg tcttatccat tctggatatg ggctatgtca ccaccacagt
      181 gccccagatg ctggtacatc tggtctgtaa gaagaagacc atatcctatg ttggatgtgt
      241 ggctcagatg tacatcttcc tgatgctggg aatcaccgag tcttggctgt ttgcaatcat
      301 ggcctatgat aggtatgtgg ccatttgcca tcccctcaga tacaaagtca tcatgagtcc
      361 tttgctgcgt gggtcactgg tagccttctg tgggttctgg ggtatcacct gtgccctgat
      421 atatactgtt tctgctatga ttcttcccta ctgtggcccc aatgagatca accacttctt
      481 ctgtgaagtg cctgctgtcc tgaagctggc ctgcgcagac acctctctca atgaccaggt
      541 agacttcatc ctaggcttta tccttctttt ggtcccactc tccctcatca ttgttgtcta
      601 catcaatatc tttgctgcta tcttgagaat ccgttcaact caagggagga tcaaggcctt
      661 ctccacctgt gtgtcccaca tcattgtggt caccatgttc tccatcccgt gtatggttat
      721 gtatatgagg cctggctctg agtcctcccc agaagaggac aagaagttgg ctctcttcta
      781 caacgtcatc tctgccttcc tcaaccccat catttacggc cccaggcaaa tcactagtga
      841 attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg gatgcatagc
      901 ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct
      961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3001 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag
     3121 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     3181 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3241 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3301 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3361 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3421 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3481 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3541 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3601 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3661 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3721 cagggttttc ccagtcacga cgtt
//
