LOCUS       sequence-203135-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli Genetic Modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pREDTKI
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggtaatgaa cctgggtccg aactctaaac tgctgaaaga atacaaatcc cagctgatcg
       61 aactgaacat cgaacagttc gaagcaggta tcggtctgat cctgggtgat gcttacatcc
      121 gttctcgtga tgaaggtaaa acctactgta tgcagttcga gtggaaaaac aaagcataca
      181 tggaccacgt atgtctgctg tacgatcagt gggtactgtc cccgccgcac aaaaaagaac
      241 gtgttaacca cctgggtaac ctggtaatca cctggggcgc ccagactttc aaacaccaag
      301 ctttcaacaa actggctaac ctgttcatcg ttaacaacaa aaaaaccatc ccgaacaacc
      361 tggttgaaaa ctacctgacc ccgatgtctc tggcatactg gttcatggat gatggtggta
      421 aatgggatta caacaaaaac tctaccaaca aatcgatcgt actgaacacc cagtctttca
      481 ctttcgaaga agtagaatac ctggttaagg gtctgcgtaa caaattccaa ctgaactgtt
      541 acgtaaaaat caacaaaaac aaaccgatca tctacatcga ttctatgtct tacctgatct
      601 tctacaacct gatcaaaccg tacctgatcc cgcagatgat gtacaaactg ccgaacacta
      661 tctcctccga aactttcctg aaataataac catggattct tcgtctgttt ctactggtat
      721 tggcacaaac ctgattccaa tttgagcaag gctatgtgcc atctcgatac tcgttcttaa
      781 ctcaac
//
