LOCUS       sequence-203298-        3607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV-driven retroviral Foxa1 expression (V5-tagged).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Roe JS, Hwang CI, Somerville TDD, Milazzo JP, Lee EJ, Da Silva B, 
            Maiorino L, Tiriac H, Young CM, Miyabayashi K, Filippini D, 
            Creighton B, Burkhart RA, Buscaglia JM, Kim EJ, Grem JL, Lazenby AJ,
            Grunkemeyer JA, Hollingsworth MA, Grandgenett PM, Egeblad M, Park Y,
            Tuveson DA, Vakoc CR
  TITLE     Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
  JOURNAL   Cell. 2017 Aug 24;170(5):875-888.e20. doi: 
            10.1016/j.cell.2017.07.007. Epub 2017 Jul 27.
  PUBMED    28757253
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07.007"; journalName: "Cell"; date: 
            "2017-08-24- 24"; volume: "170"; issue: "5"; pages: "875-888.e20"
COMMENT     Sequence Label: MSCV-Neo-GFP/V5-Foxa1
FEATURES             Location/Qualifiers
     source          1..3607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    118..139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(154..159,160..177,178..184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             370..884
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(1085..1104)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1232..1254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1448..1467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1567..1589
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1627..1645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1713..1817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1818..1886,1887..2678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2036..2055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2849..3437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3338..3357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
       61 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      121 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      181 gttgtgtgga attgtgagcg gataacaatt tcacaattct ccccagctct ttctgcgctg
      241 tccataatga actatttcag gagtattgga gtatgtggat tcaaaacagt ggctaattga
      301 gtcaggatta cttaatattc gactcctacc ctcagaatgt tgttctccaa cttcaagaaa
      361 ctgttaccca tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca
      421 aggaacagcg agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta
      481 cccgggcgac gcagtctatc ggaggactgg cgcgccgagt gaggggttgt gggctctttt
      541 attgagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
      601 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
      661 ctagaaactg ctgagggcgg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
      721 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttctgctgtc tctctgttcc
      781 taaccttgat ctgaacttct ctattctcag ttatgtattt tccatgcctt gcaaaatggc
      841 gttacttaag ctagcttgcc aaacctacag gtggggtctt tcattccccc ctttttctgg
      901 agactaaata aaatctttta ttttatctat ggctcgtact ctataggctt cagctggtga
      961 tattgttgag tcaaaactag agcctggacc actgatatcc tgtctttaac aaattggact
     1021 aatcgcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     1081 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     1141 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     1201 atctccttgc atgcaccatt ccttgcgccg tcgttttaca acgtcgtgac tgggaaaacc
     1261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     1381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     1441 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     1501 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1561 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3601 tgagcga
//
