LOCUS       sequence-20338-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p4949 pLPCX-His-Xpress NLS hBrd4 CTD
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..90
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(94..102,103..117)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcgagctaa gcttcgaatt cccatggggt ggttctcatc atcatcatca tcatggtatg
       61 gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggta
      121 ccaggatccc ctaagaagaa gaggaaggtt cggcaccaca agtcggaccc ctactcaacc
      181 ggtcacctcc gcgaagcccc ctccccgctt atgatacatt ccccccagat gtcacagttc
      241 cagagcctga cccaccagtc tccaccccag caaaacgtcc agcctaagaa acaggagctg
      301 cgtgctgcct ccgtggtcca gccccagccc ctcgtggtgg tgaaggagga gaagatccac
      361 tcacccatca tccgcagcga gcccttcagc ccctcgctgc ggccggagcc ccccaagcac
      421 ccggagagca tcaaggcccc cgtccacctg ccccagcggc cggaaatgaa gcctgtggat
      481 gtcgggaggc ctgtgatccg gcccccagag cagaacgcac cgccaccagg ggcccctgac
      541 aaggacaaac agaaacagga gccgaagact ccagttgcgc ccaaaaagga cctgaaaatc
      601 aagaacatgg gctcctgggc cagcctagtg cagaagcatc cgaccacccc ctcctccaca
      661 gccaagtcat ccagcgacag cttcgagcag ttccgccgcg ccgctcggga gaaagaggag
      721 cgtgagaagg ccctgaaggc t
//
