LOCUS       sequence-203756-       13836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 13836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: ath5:mKate2-aPKC-CAAX
FEATURES             Location/Qualifiers
     source          1..13836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1389..1405
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1390..1406
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1440..1456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6392..6408)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6445..6463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6463..6487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6490..7185
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(9062..9086)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9091..9225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9115..9134)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9169..9188
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11366..11553,11554..11565)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11599..11615)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11652..11670)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11691..11707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11691..11707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11704..11726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11715..11731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11739..11745,11746..11763,11764..11769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11784..11805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11922..11939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12093..12681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12173..12192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12852..13643,13644..13712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13475..13494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13713..13817)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg
     1381 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1441 atccactagt tctagaattt aaatatttta aaaatgtata tatataataa atgtatatat
     1501 atataaatgt ataatgagca aaattctaag ttttatgcaa ataaaaaaag tattatggaa
     1561 aatgtattat attgcagttc taaattattt aagaaaacgt ggctgacttg ccaaaatgat
     1621 ggtaaactgt gcaactctgc ttccaagatg ttagaaacag acgtttttct tttagcctca
     1681 taatttaatg ctgttgcgat gttaccgtct cttcgctgta ctggttgtta ccaggttacg
     1741 gaaaaatata acatgctcac aacatccaat cagattaaat gaactgaaaa gcaatatggg
     1801 ttttaagaca tcacagagaa ggtaacattt aaaggcttaa aagcacaaac tgtttctcgc
     1861 ttctaagact aagtaagtaa gtttttgcac acaattaaca aatagttgca ggcaaattaa
     1921 actttagtga caaggcagta ctggcccgat caggccagta atgattttgt ctactgtcca
     1981 aagcatctct cacgctggcc ctgggtcatc aagcagttct tattgttgag ccctgcatta
     2041 taattatata caattttttt tttattattt aattttttta aattaaaatg ttatgtaatg
     2101 ttatgttaat aaatgtttaa ataagaatat taaaaaataa tttgatttaa tacaaataca
     2161 gggaagcacg ttttaaaaag taaataaatt ttgttaaatt ttatataagg aaaatatgtt
     2221 tatttctgta tttttctata cactgaacac aactaagaat ttctaaataa atatagacaa
     2281 atgctattag gcatgggatg ataaccagtt taaaggtcaa ggtttaaaaa cccccagaat
     2341 tatctgttat accgttccaa cgatatatgt aattttttta ttaacacttt taataaaaaa
     2401 ggaccctcat catcaaactg ttcttaaaat aaactatatt gtgctcaaag gtaaaaacag
     2461 ttcttgtttt ttacccagac atttaaaaaa agaatttatt ttagagcagt aatcacaata
     2521 ccgtccgtga taccgtgatt tttttttatc aaaggtaatc atacagtcac aatcttttac
     2581 cggcccatgc tatttatacg gcagtgtaaa ataaagtgta aattgtaacg cgttataaag
     2641 aattgcttct tttaaacaat gccacaaaaa ttatgaacat tttttttttg taaaaatttt
     2701 aaaccaaaaa taactaaaaa tttctccaaa tataaaacaa ttgagcaggg cacattgtaa
     2761 cacataacga aaactgcttc cattgttttt ttttatactt gaaaatcctt ttcatctttg
     2821 tcccacataa gtcataacat taacaataat aattgaatat agatttcttg tctaattgac
     2881 tgcccttaat aagacattgc ttcattgacc agttaagatt aaacataaca accagaaggg
     2941 aacaaaaagg ctcaaatagg catgaaatta caagcacctt gcttgaccta attacagtga
     3001 cgagcctcat ctcaccagat gccccaatct gacggtgggg gttcaagtct cctcatctat
     3061 ggaggtcact gtttacacaa tatgcctcac ttttctgaat gacctcattg gtcaatatgc
     3121 cagttctgtg agaatcgatc aaaaatagct ttaaagatta aagattttaa gttttcttct
     3181 cactttttta ggtagattat gtgttgaaat gaacacaaca gtttataccc atatctcaga
     3241 gatcatatcg atgatgaact ttaatgaatg ataagttatt atattatata ttaaatcata
     3301 ttatattata atatattata ttctatattc gttttgatag atgtagtgtt taaatgaaca
     3361 gtttatactc atctcaagga tcataaagaa gtgtcatatc atagaactgt aaatgacaat
     3421 gttatattat tttgatggac ctttagatat attctttggt tattatactg tatatttttg
     3481 gtagattatg tttacatcat agagctttaa atgactagtt ctattctagt ctatttgatt
     3541 tttttttttt taaatgacca caacagttta tactcaaata tcagtgatca taatgttata
     3601 tcaacatata aaatctatat tatgttatat tatattatat tatattatat tatattatat
     3661 tatattatat tttattttat attagacagc acccattctc agttttacat ctattatttg
     3721 actgtcagct tgacttaagt aggagtttga tcattagtcg tcactttcct cgagttcaga
     3781 ggattacgca ttcgattttt aagaccattt tctccttcgg aatgaacggc gctttatgct
     3841 ttaaatgaaa accagattta ctaaatcctg gattaagctg ttaaactgat aatagacctt
     3901 ctcactttcc cctcgtcaag accgagtaaa tctttaatct gctcaacact gtctcaaaaa
     3961 ttacaggagt agcttgtaac atatggcctt ttgcatagga gtccagacaa gcctatcatg
     4021 tgaacgagtg aaaaaacaag gccgtttaac atgtctggac caagcccttt aaacgggaca
     4081 agcagcagct ggtcagggat gccaatctgc aatcaatgaa aacaaactaa accagtggat
     4141 tgggtttata tgcccaattg aacagcagaa ttactcatct aatgcagatg agcgtatatg
     4201 gcttgatggc cacagtcagc cgggacatga attatgcaga agagatatag cgatttgtca
     4261 cttgtacaca aatcagatgt aataatgtat gtatttagtg cagtatttta tatagaacgc
     4321 tactttacga tttcaagtac ctgtaggaaa atatgttata gataattact atactataga
     4381 ggtgataatt cataatgcat aactttaaaa acattttgtt tcatagtata gcaattatat
     4441 atacagtata taagatagac tgcatatata tatatatata tatatattat atctgtgtgt
     4501 tattatatta taaccttagt ataacaataa tagtattttg gaaaggttta tgaatgagtt
     4561 tttttgccaa caggcttttt ttggaggggg tgaaactgtg actgtctgaa tctgctttgt
     4621 ttgattcaca ttgaacagat tgagatcgac agcttgtcga agacttctgt ctgtctcaat
     4681 tcctctgaga cactcaaatc aattatttag tgctgcccca caaatgtgtt ttatatggtt
     4741 ttgttattgg tttactatat attattaaat atatagaatg taatatgtaa tatagagaaa
     4801 tgtgctgatg atacagtaga tgaatactga cactatatta aaatattcta tataaaagta
     4861 taaaatttga acactgacgc tatattaaaa gattgcaaat gaccgtataa atgtttaaaa
     4921 tgtagaaact tcacatgtta ttttttctaa acaagcatgc tggttattac tttataatta
     4981 tgtaacaata taactgtact aatacagttc agtaattaac aagtaattca aaaatatttt
     5041 tattttaaat gtttatattt tatcattatt cttcaagcca ctaggttata ttagtttgtc
     5101 agcaaaaatg aacaaattag caccaacgct tataatttaa aatattaaat cttgaaaata
     5161 ttttaaagaa tgttacattt aatgaaaatt attttaaaaa gcataagaaa aattacacac
     5221 tcaaattgca tttaatatgc tttccgtgta aattacttat gtaaatttaa tttgatgcac
     5281 actaaaatgt ctcgtctatg acctatatac ataatgtatt atcatattgt aactcaaata
     5341 ttacatgtaa tattaatatt accttaaata tttatatatt acaatttaac attatgaata
     5401 acaaagacaa ctggagtatg tttgaccaaa acaaaacaac aatctttcta tttgtaaaag
     5461 ttaattttaa aaaaactgtt gcgatgtgta aaatatccat ttactttatt cagtaaaact
     5521 aatgtggatt ataatagtat agcataaata tataacaata tatcaattta atattaacat
     5581 caatataaaa ttgatgaatc tatctatgaa aaaatatgaa aaattcatta tttactcact
     5641 tattactaat tgcacacccg tatactattc ttttttcatt gaacacaaag aaagatatgt
     5701 tgaagtatgc tgaaaaccgg taaccattga cttcaacagt atttattttt ccaatggcag
     5761 ccagtggtta caggtttgca gctttcaacc aaatattttc ttttgtgatc agcagaagaa
     5821 agaaactcat aaaagtttgg aaccacttaa ataaaatagt gcataaatag tctgtaaatg
     5881 ttcatttttg tatgaactat cccttcaaat tatcgtttat attaaaagat tccagtaata
     5941 aatgtaaata atgagaccta caaccctgta aaacaaattg caaaatcaaa ctgactctat
     6001 acaggcaatt actcctgcaa acaatgttct catttaagaa atccaaagat gtcacggttg
     6061 cttaaagatg catgcacttt ttaattaggt aatggtcctt gccacccggc gctggttgtg
     6121 gccagatggt aagttatctc tggtttgtaa cgcctgtttt cagtgataga gcagccaaag
     6181 ttaattggaa tgtcattaaa gtttgagtta ctgggggttg gggctggact gcactagtcc
     6241 tgctgttgga gatgtgcgtt tcattaataa taatggatgt ttggatggag taataagggt
     6301 ggggctccac ctgctgccac ctgttaatac tctatataag catcgctcat ccttatggca
     6361 gaacagaagc gattaagaac catccggggg ggatccacta gttctagagc ggccgccacc
     6421 gcggtggagc tccagctttt gttcccttta gtgagggtta attcaagttt gtacaaaaaa
     6481 gcaggctgga tggtgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     6541 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     6601 ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac
     6661 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc
     6721 cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac
     6781 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     6841 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     6901 acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg cctggaaggc
     6961 agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc
     7021 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     7081 agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
     7141 gctgtggcca gatactgcga cctccctagc aaactggggc acagagatcc tgcctttctt
     7201 tctagatata aggttgccat gcccagcagg acggacccca agatggaccg gagcggcggc
     7261 cgcgtccgtc tgaaggcgca ctacggcggg gacatcctga ttaccagcgt ggatgccatg
     7321 acaacattca aggacctctg tgaggaagtg cgagacatgt gtggcctgca ccagcagcac
     7381 ccactcaccc tcaagtgggt ggacagtgaa ggtgaccctt gtactgtgtc ctcacagatg
     7441 gagctggagg ccttccgcct ggcctgtcag ggcagggacg aagtgctcat catccacgtt
     7501 ttcccaagca tcccagaaca accgggcatg ccttgtcctg gagaagacaa gtccatctac
     7561 cgccgtggag ccagaagatg gaggaagcta taccgagcca acggccacct cttccaagcc
     7621 aagcgcttta acaggagagc gtactgtggc cagtgcagcg aaaggatatg gggcctcgcg
     7681 aggcaggggt acaggtgcat caactgcaag ctgcttgtcc ataaacgctg ccacgtcctc
     7741 gtcccgctga cctgcaggag gcatatggat tctgtcatgc cttcccaaga gcctccagta
     7801 gatgacaaga acgatggtgt agaccttcct tcagaagaaa ctgatggaat tgcttatatt
     7861 tcttcatctc ggaaacatga caatatcaaa gatgattctg aggaccttaa gcctgtcatc
     7921 gatggggtgg atgggatcaa aatctctcag gggctggggc tgcaagactt tgacctcatc
     7981 agagtcatcg ggcgtggaag ctatgccaag gtcctcctgg tgcggttgaa gaaaaacgac
     8041 cagatttacg ccatgaaggt ggtgaagaag gagctcgtcc acgacgatga ggatatcgac
     8101 tgggtgcaga cagagaagca cgtgttcgag caggcatcca gcaacccctt cctggttggc
     8161 ttacactcct gcttccagac aacgagccgg ttgttcctgg tcatcgagta tgtcaacggg
     8221 ggggacctca tgttccacat gcagaggcag aggaagcttc cagaggaaca cgccaggttc
     8281 tatgctgctg agatctgtat cgctctcaac ttcctacatg agagagggat catctaccgg
     8341 gacctaaaac tggacaacgt cctcctcgat gccgatggac acattaagct gacggactac
     8401 ggcatgtgca aggaaggcct aggccccggc gacacaacaa gcactttttg tggaaccccg
     8461 aactatatcg cccccgaaat cctgcgagga gaagagtacg ggttcagcgt ggactggtgg
     8521 gcgctgggtg tccttatgtt tgagatgatg gctgggcgct ccccctttga catcatcaca
     8581 gacaaccctg acatgaatac tgaagactac cttttccaag ttatcctgga aaagccaatt
     8641 cggattcccc gtttcctgtc tgtcaaggcc tcacacgtct tgaaaggatt tttaaataag
     8701 gatcccaaag agaggcttgg ctgccggccg cagactgggt tttccgacat caagtcccat
     8761 gccttcttcc gaagcataga ctgggacctg cttgaaaaga agcagaccct gcctcccttc
     8821 cagccccaga tcacagatga ctatggcctg gacaacttcg acacgcagtt caccagcgag
     8881 cccgtacagc tgaccccaga tgatgaggac gtcataaaga ggatcgacca gtccgagttc
     8941 gaaggcttcg agtacatcaa cccgcttctg ctgtctgctg aggagtccgt gctcgagaag
     9001 ctgaaccctc ctgatgagag tggccccggc tgcatgagct gcaagtgtgt gctctcctaa
     9061 gacccagctt tcttgtacaa agttgatgta gatccagaca tgataagata cattgatgag
     9121 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     9181 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     9241 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     9301 gcgccaatgc attgggccct cgagcatatg agatctgtcc cgatctttct cttctgtgct
     9361 gtcaatttag agtagttttt gaggtaattt gggtgcattc tctgtaaaaa caacaaataa
     9421 atatttatat tgtatgcaat aaatgtaaca attacacata cactctctga ttacagcatt
     9481 aaagggttag ttcacccaaa aatgaaaaga tgtcattaat gactcaccct catgtcgttc
     9541 caagcccgta acacctccgt tcatcttcgg aacacagttt aagatatttt agatttagtc
     9601 cgagagcttt ctgtgcctcc attgagaatg tatgtacggt atactgtcca tgtccagaaa
     9661 ggtaataaaa acatcaaagt agtccatgtg acatcagtgg gttagttaga attttttgaa
     9721 gcatcgaata cattttggtc caaaaataac aaaacctacg actttattcg gcattgtatt
     9781 ctcttccggg tctgttgtca atccgcgttc acgacttcgc agtgacgcta caatgctgaa
     9841 taaagtcgta ggttttgtta tttttggacc aaaatgtatt ttcgatgctt caaataattc
     9901 tacctaaccc actgatgtca catggactac tttgatgttt ttattacctt tctggacatg
     9961 gacagtatac cgtacataca ttctcaatgg aggcacagaa agctctcgga ctaaatctaa
    10021 aatatcttaa actgtgttct gaagatgaac ggaggtctta cgggcttgga acgacatgag
    10081 ggcgagtcat taatgacatt attttcattt ttgggtgaac taacccttta ataaaactga
    10141 catttgcagc caattgcaca ctttataatg ttaaatactt gttgatttta gaaatacagt
    10201 agcatttagt gacaatcaaa cattaatttt atttctgatt atattgacaa aaacacagga
    10261 ctgtaaggct gacatgacag ggtgaattga tttcattaca ttagttttga tggtaacact
    10321 tttacaataa ggttcgttag ttaactacat tagttaacat gaactaataa tgaactgcac
    10381 gtatacatgt taatttcaac atttactaat actttatatc ttgttaacat tagttaatgc
    10441 actgtgaact aacatgaaca aacaatgaac atctgtattt ctattaacta acgttaacaa
    10501 agattaacag atacagtaac aaatgtattg ctcatggtta tttaatgtta gttaatacat
    10561 gaactaatca acctcattgt aaagtgttac cgttttgaca atacaacatg tttggaaaaa
    10621 aatttctcgc ttggttttct tgtgttttgc atgaattcaa acacaatgag agcacggaat
    10681 gataatgatt attcaaagct gcaataggcg ccaaatttcc cgtggaccta acgttaccaa
    10741 ttacaaatgc attttagcat tttattacgt gagccttata ttgtcaatcg catgcattag
    10801 tgaaaatagg cctactacac atataaaagg cgcgccatca acgtcaaaac atgcacaccc
    10861 aaaaaaaaaa aaaaaaaaaa aaaaaaaaac tatcagtaaa acaaaatact taatgtactt
    10921 acctcaatat gcttccttag gtttgatggc gaacttttga aggccgatat ttctttatta
    10981 agcgggagac agaggacaca tttcatcttg aatgaatctt tatttacacc acttaaagaa
    11041 aagaattcgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
    11101 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
    11161 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
    11221 gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt
    11281 tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt
    11341 ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
    11401 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
    11461 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
    11521 aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta
    11581 ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
    11641 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
    11701 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11761 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11821 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11881 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11941 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12001 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12661 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12721 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12781 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12841 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12901 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12961 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    13021 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    13081 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    13141 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    13201 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13261 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13321 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13381 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13441 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13501 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13561 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13621 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13681 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13741 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13801 caaatagggg ttccgcgcac atttccccga aaagtg
//
