LOCUS       sequence-203865-        8874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_04g07810 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_04g07810-bio-His
FEATURES             Location/Qualifiers
     source          1..8874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4038..4055
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4107..4162
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4161..4180)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6398..6416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6484..6588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6589..6657,6658..7449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6807..6826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7620..8208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8109..8128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8362..8379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8496..8517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8532..8537,8538..8555,8556..8562)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8570..8586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8575..8597
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8594..8610
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8594..8610
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gcatcatcag cgacgtgaac gtggaaggcc tggccaagaa gatctacctg gacgaggacg
     1261 gcaacgagat cctggaaaaa gtgtgctact tcgaggccgt gaccctggaa agccacaact
     1321 gcgacgtgta cctgcggccc aacgagcggc tgagcgtgaa gtgcagcgcc accgccaaca
     1381 tggacagcag cgccgtgcct gtgggcatca ccctgcagag cgacaacagc aacgtgaact
     1441 tcgagagcac ctgtaaagag gaattctgca ccgccaagtt cgagtacctg cccaacaagg
     1501 cccacaacaa cgtggccgtg aactgctacc tgaaagaaag caccgagaca gtggacccca
     1561 cccaccccaa cgtggaaaac atcttcatgt acatcaacgt gaagctgtac gacgccaccc
     1621 tgaagaacgt gatcgacgac tacaccctgt acgagaacaa gatcttcgac gtgacactgg
     1681 acgacgtgac cctgcgggac atcaacctgg ccaccctgga attcggcgag aacatcctga
     1741 gcacccggac ccccggcatc aacgacgcca agaacagcga cgacaagagc ctgaccgtgg
     1801 accacaccga cgaccgggtg gccctggaag ccaagcccaa ctacaacgac tgccactgcg
     1861 acttcaccaa cagcgagcgg gccgagtaca agaacaagtt ctacaccggc atcctgcacc
     1921 ccaaagagcg gttcatctac cggtgcagca accccgccga caagcccgag atcgtgacct
     1981 tccccagaat ccagaccaac ggctacgtga tggaacagct ggaaaacggc cggaacgtgc
     2041 tgaaagagat ccccctggac gacatcagaa agggcctggc cagatccgag ggcctgctga
     2101 tgtacgacta cttcgacgac aatctggcca agtacaacga gatctatctg atctgccaca
     2161 ccaccgacgt gtccaccccc gtgaccaagc tgtccaccct ggatggcatc atccggatca
     2221 tcctggccta cagcggcgtg cacctggacg aaaagaccgg cgtggtcaac ttcgccgact
     2281 tcaagatcgt ggccagcgac gaggaagaga gcggcaacgg caagatcggc tactacacca
     2341 tcaccaacga ccagcccagc accatcttcc agtgccccag caccgaaacc gtgaagtaca
     2401 agctgctgcc cgagcggcac aaccacgtgt actacaagat gccccagatg gaaaagaacc
     2461 ccttcaagaa cgacaagtac aacccccagg gcgacatcgg ctacgccatt gccatgaagg
     2521 gcatctccat catcaagaac gaggaccgga tcaccatcaa caaggacggc tacagcaccc
     2581 tggcctacgg catgaacccc ttcgtgtact tcctgtgcgt ggtgcccaag cagaagaaag
     2641 agaacagcct gcacaaccct gcccccgacg ccgtgaccga ggacggcgtc gagaaggtgg
     2701 tgcagagcat cgacgacgcc gagacagaca agagccctgg cgaagaggac cccatcgacg
     2761 atgacagcat caccgaggac tgggaccgga agtacggcgc catcatcatc ggcctgagca
     2821 cctacagcaa cctgcggaag ttccggatct gcggccctgg ctacagcagc ttcgactaca
     2881 acggcgtgta catcgaggac gtggactgca actacgcctt caacgcccct cccatcagcc
     2941 tgagcaacag cctgatcgac agcggcatcg agaacgaggg cagccagatc aagagcaccg
     3001 tgggcatcga cagccccgag gaatccaagc ccgtggacaa gagcgacagc aagctggtgg
     3061 aagatagcga cagcagcgag cagaaagagg acagcgactc cggcatcgac tcccccatcg
     3121 tgcagatgtg ccccatggac aagttcaacc ctaacggccc cagatgcatg accgcccaga
     3181 aaggcttcat gggcatgaag cgggccaact tcatcaagga tttcttcgag cccgtgaagg
     3241 acgtgctgag cgtgtccagc ctgtggattc tggaaaaaga gcgctgggag acattcgaga
     3301 agtccggcgt gaagtccgtg acctgcctgt gctacgacag cgtgggcgac gtgatctcca
     3361 agctggtcgt gaacgacttc aactctggcg cgccgtcgac ctccatcacg gcctataaga
     3421 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     3481 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     3541 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     3601 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     3661 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     3721 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     3781 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     3841 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     3901 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     3961 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     4021 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     4081 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     4141 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     4201 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     4261 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     4321 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     4381 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     4441 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     4501 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     4561 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     4621 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     4681 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     4741 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     4801 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4861 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4921 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4981 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     5041 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     5101 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     5161 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     5221 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     5281 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     5341 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     5401 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     5461 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     5521 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     5581 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     5641 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     5701 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     5761 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5821 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5881 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5941 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     6001 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     6061 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     6121 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     6181 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     6241 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6301 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     6361 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6421 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     7141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7741 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7981 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8401 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8461 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8521 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8581 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     8641 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     8701 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     8761 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     8821 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
