LOCUS       sequence-204099-       10932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine phospho-mimetic CEP215 at S608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10932)
  TITLE     Expresses murine phospho-mimetic CEP215 at S608
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: (PM-S608D)FLAG-Cep215-FL
FEATURES             Location/Qualifiers
     source          1..10932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..49)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        221..600
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        601..804
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     755..775
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     849..868
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        849..867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      881..890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             890..961
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     promoter        complement(6471..6489)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6472..6489)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6509..6526)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6515..6739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6785..7213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6872..6891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7082..7103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7222..7242)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7227..7556
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7407..7542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7469..7488
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7623..8417
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7677..7696)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8287..8306
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8591..8712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8628..8647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8682..8701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8761..8777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8761..8777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8774..8796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8785..8801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8809..8815,8816..8833,8834..8839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8854..8875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8992..9009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9163..9751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9243..9262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9922..10713,10714..10782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10545..10564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10783..10887)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
       61 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
      121 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
      181 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
      241 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      301 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      421 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      481 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      541 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      601 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      661 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      721 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      781 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      841 atcgaaatta atacgactca ctatagggag acccaagctt gccaccatgg attacaagga
      901 tgacgacgat aaggattaca aggatgacga cgataaggat tacaaggatg acgacgataa
      961 gggatctatg gactcgggga tggaagagga aggcgccctg cctgggaccc tcagcggttg
     1021 cagtggcctt caccctgttc tgccaagtga cctggatgtc atcagtgaca cttctggctt
     1081 gggaaatgga gtgctcccaa gcatgtcaga agaaaaggtt tctcccacca gagcccggaa
     1141 catgaaggac tttgaaaacc aaatcactga gctgaagaag gagaacttca atctaaagct
     1201 ccggatctac ttcctggagg agagaatcca gcaggagttt gctggcccca ccgagcacat
     1261 ctacaagacg aacattgaac tcaaggtgga agtggaaagt ctgaagcgcg agctccagga
     1321 gaaagatcag ttgctcgtca aagcctccaa agcggtggag agcctagcag aacggggtgg
     1381 ttctgaagtc cagcgggtga aagaagatgc aaggaagaag gtgcagcagg tggaagacct
     1441 cctaacgaaa aggatacatc ttctagaaga agatgtgaaa gccgcccagg cagaactgga
     1501 gaaggccttt gcggggacag aaacagagaa ggctcttcgg ctcagcttag aaagcaagct
     1561 ctcggcgatg aagaagatgc aggaggggga cctggagatg actctggctc tggaagagaa
     1621 ggacagactg attgaggagt tgaagctgtc tttgaagagc aaagaagctt taattcagtg
     1681 ccttaaagag gaaaaatctc agatggcatc tcctgacgag aacgtgtcat ctggagaact
     1741 ccgaggactt tctgccactc tgagggaaga aaaagagaga gacgctgagg aatggcagaa
     1801 ggagagaaac cactttgaag agaggatcca ggcacttcag gaggacctga gagagaagga
     1861 aagagaaatt gctacagaga agaaaaataa cttaaagagt tataaagcca ttcagggtct
     1921 aaccatggca ttaaaatcaa aggaaagaga ggttgaagaa cttgattcca aaatcaaaga
     1981 ggtcactacg gactccacca aaggcagaga ggatccctta aaaacccaga tccccagatt
     2041 ccagctaaga gaggggtctg aggactgtga agctgcgctg gtagagaagg aagccctatt
     2101 ggccaagcta cactcagaaa atgtcacaaa gagcactgag aaccataggt tgctcaggaa
     2161 tgttaaaaag gtcacccagg agttgaatga cttgaagaag gagaagttga gactagagca
     2221 ggccctggag gaagcccacc aagaggggaa cagaggagcc cgaaccattc acgaccttag
     2281 aaatgaagtt gagaaactac gcaaggaagt gagtgagaga gagaaggctg tggaaaaaca
     2341 ttacaagagt cttccaggtg aaagcaagac aaagtttcat actcaagaac aagtggtcag
     2401 aagtctgaca ggcagtggca gtcaggagga cctgctgctt cagaaatcca atgaaaaaga
     2461 tttggaagca atacagcaga actgctattt aatgactgca gaggagctca agtttgggag
     2521 cgatggctta ataacagaaa agtgttctca gcagtcacca gacagcaaac tcatcttctc
     2581 caaggaaaag caacaatcag agtatgaagg gctgactggg gacttaaaag cagaacagaa
     2641 catctatgcc catctggcaa agactcagga cacagacagt gtcagcaacc tgcaggctga
     2701 gctgaaagag gtccttgccc ttcggaagca gctggagcag gatgttcttg cctaccggaa
     2761 cctgcagaag gccctgcagg agcagctcga tgagattcgg agtcgagaag aagagccgtt
     2821 ttcattttat agcgatcaaa catcgtatct gagtatttgc cttgaagagc acaatcagtt
     2881 ccaattggag catttttctc aagaagaact caagaaaaag gtcagtgacc tcatacagct
     2941 tgtgaaggac ctgcacacag acaaccagca cctgaagaaa accatttttg atctctcttc
     3001 tgtgggtttc cagggaagtg acagacttga gttaacaaaa caagaagagc ttgtggctag
     3061 caaggaggat gaggacacac tcaaatttga agcggacgtt gagactcctt tccagagtga
     3121 ccagcatttg gagcaaagtc gtgagatcat ggaggactat gctgagggtg gctgcaaaag
     3181 tggatatgga aggcacatgg attccaatat tttaggtcat gatggggccc aaactcctgg
     3241 tgcctctgaa gagcacaccc tagaagatga gctcctgggc ctgcttgcca ctctcttcag
     3301 taagaaggcc acaccgttgc tggaatccag gccagatctt ctgaaagcac tcggtgccct
     3361 acttttggaa cgcatatgct tggcagagca gggtcgtcct ggagatcacc ttgatagtaa
     3421 aactgagaag gcacttcagc aagtagctgt tcagttgaga gatgaacttg gccattcctt
     3481 ccctgccaac tcgttctcca agtcatacaa tgaagtcaag tcaatgtggg gaaattggct
     3541 ggtaaagaca ggagacgaag acacggtgga gctgaagagt gtttcagtgc agaccatggc
     3601 catagaggac accccgcatg gattcaaacc tcaaagcaag agagatgctt gggcagagaa
     3661 acaagaagag gccattttca gcactgagct tgagtcagag gcgttgggtg agatgccagg
     3721 gcaacaggcc actcacctct cattccccag tgccatcaac ccggatgccg agaagacggg
     3781 cttgttgatc caattaaaga cacctgagtt attggaaaat ttatataatc ttcctgcttc
     3841 gccagaggtg gtggtggctc aactccaagg ccaggttcta gagctgcaga gggagctgaa
     3901 agaattcaaa acccgcaaca agcaactcca tgagaagcta attctggcag aggcaatgat
     3961 ggaggggctg ccggtgccca actctgcgct ggtgaatgtg cctgcagccc aggctgttgt
     4021 gagaacagcc ttccaggaca acccaggcga gcaagaagga cctgaaacca cacagtctgc
     4081 tgggagagac aaggacatgg acagtgacca atatacaagc tttgagatcg attctgaaat
     4141 ctgtccacct gatgaccttg ccttattacc agcatgcaaa gagaatcttg aagacctcct
     4201 aggcccatcc tccatagcca cttacctgga ttccaagagt cagctctctg ttaaagtcag
     4261 cgtgaatgga actgaccagt ctgagaatat caatatccct gatgacacag aagacttaaa
     4321 acagaaaatc cacgacttgc aaaccgagct ggaaggctac cggaacatca ttgtccagct
     4381 actgaagcac tctcagtgca gtgaggctat tatcacagtt ttgtgtggga cagaaggggc
     4441 ccaagatggc ttgaacaagc ctaagggcca tattgatgag gaagagatga cattttcaag
     4501 tttgcaccaa gtacgatatg tgaagcatat gaaaatcctc cgtccactaa ccccggagat
     4561 gattgatggc aagatgctag agagcctcaa gcagcagctg gtggaccagg agcaggagct
     4621 acagaaggag caggacttga acttggaact tttcggtgaa atccatgacc tgcagaacaa
     4681 gttccaagac ctctcgccat caagatacga ttcattggtt cagtcccaag cgagggagct
     4741 ctcccttcag cgacagcaaa ttaaggatag ccatggcatc tgtgtcattt accgtcaaca
     4801 catgagcacc atgatcaagg cttttgagga gcttctgcag gccagtgatg tggacagttg
     4861 cgtggccgag ggcttccggg agcagctgac acagtgtgct ggactgctag agcaactgga
     4921 gaggctgttt ctccatggaa aatcagccag agtggaacca catccccaga atgaactgct
     4981 caaggggtta aggactgtgg aaggcaactt accctaccac catctcctgc ctgaatctcc
     5041 tgagccttct gcctcccatg ctctgtcaga tgatgaaatg tctgagaagt ccttcctgtc
     5101 tagagacccg aagccagaca gcgatactga gaagtaccca gcaatggcga gccattttcc
     5161 tcaagactta ctaatggaac acatacagga aattcgaact ttgagaaagc atttggaaga
     5221 atctattaaa acaaatgaga agctccggaa acagctggaa cggcaggggt ctgagactga
     5281 ccaaggctcc agaaatgttt ctgcttgtgg cttagcactg cacagctctc tgacgtcaga
     5341 gatacatttc ctgaggaagc agaacgaggc cctcagtatg atgctcgaga aggggtccaa
     5401 agataaacag aaagagagtg aaaaattacg tgagtccctg gctaggaaag ctgagagtct
     5461 tgagcaactt cagctagagt acaccagtgt aagggaagaa aatgaaaggt tacagagaga
     5521 catcattgag aaggagagac acaaccagga gctgactgag gaggtctgca gcagccgcca
     5581 ggaactgagc agggtgcagg aagaggcgaa gtcaagacag cagctgctct cacagaagga
     5641 caagctgctt cagtccctcc aaatggagct caaggtgtat gagaaactgg ccgaggaaca
     5701 tccgaggcta cagcaagatg gcagcaaatg tcctgaggcc agcgacaatt catttgatct
     5761 atttgaatcc acccaggcaa tggcacctaa atcagcttct gagactcctc tgctctcagg
     5821 aactgatgtt gactcgctct cctgtgacag taccagttct gccaccagcc ctactagcat
     5881 gccctgcctg gttgctggcc accacatgtg ggccagcaag agtggccacc atatgctggg
     5941 cctgattgag gactatgatg ccctctataa gcagatcagc tggggccaga cactgcttgc
     6001 caagatggat gttcaaaccc aagaggctct gagccccaca agtcacaagc tgggaccaaa
     6061 gggttcttca tccgtgcctc tgagcaagtt tctttccagt atgaacacag ccaagctggt
     6121 cctggaaaaa gcatccaggt tgctgaagct cttctggagg gtctcagtcc ccaccaacgg
     6181 ccagtgttcc cttcactgtg agcagattgg agaaatgaag gcagagaaca cgaaactaca
     6241 caaaaaattg tttgaacaag aaaagaagct gcagaacaca gcaaaactat tgcagcagag
     6301 caagcaccag gagaaagtca tctttgatca gttggtcatc acccaccaag tccttcggaa
     6361 ggccagggga aacctggagc tcaggcctgg ggccacccgt ccaggagcat ccagtcccag
     6421 cagaccgggc tcatgagcgg ccgctcgagc atgcatctag agggccctat tctatagtgt
     6481 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6601 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6661 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6721 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6781 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7021 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7081 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7141 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7201 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7381 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7561 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     7621 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7681 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7741 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7801 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7861 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7921 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7981 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8041 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8101 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     8161 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8221 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8281 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8341 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8401 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     8461 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8521 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8581 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8641 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8701 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9601 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9721 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9781 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9841 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9901 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9961 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10021 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10081 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10141 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10201 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10261 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10321 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10381 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10441 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10501 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10561 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10621 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10681 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10741 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10801 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10861 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10921 cggatcggga ga
//
