LOCUS       sequence-20428-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..164)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(172..188)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(172..188)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(194..457)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(277..300)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gcataaagtg taaagcctgg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
       61 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      121 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ggatccacta
      181 gttctagaat ccgtcgaaac taagttctgg tgttttaaaa ctaaaaaaaa gactaactat
      241 aaaagtagaa tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg
      301 tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt
      361 ttttcagctt tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga
      421 tagatacatg cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac
      481 tccattgagg ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta
      541 agagaatgga cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc
      601 tttttttttc tggatgccag cttacaaagc gggctccatt atattt
//
