LOCUS       sequence-204386-        9792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Drosophila ISWI C-terminally tagged with Combined Sce 
            VMA intein and chitin binding domain (iCBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Khuong MT, Fei J, Cruz-Becerra G, Kadonaga JT
  TITLE     A simple and versatile system for the ATP-dependent assembly of 
            chromatin.
  JOURNAL   J Biol Chem. 2017 Nov 24;292(47):19478-19490. doi: 
            10.1074/jbc.M117.815365. Epub 2017 Oct 5.
  PUBMED    28982979
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.815365"; journalName: "J Biol Chem"; date: 
            "2017-11-24- 24"; volume: "292"; issue: "47"; pages: "19478-19490"
COMMENT     Sequence Label: pET24-ISWI-iCBD
FEATURES             Location/Qualifiers
     source          1..9792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     183..202
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     286..305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        286..304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    305..329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             344..366
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3464..4282
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             4738..4749
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4859..5014
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     terminator      5026..5073
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5110..5565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5197..5216)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5407..5428
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5657..6472)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5657..6472)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6380..6399
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6464..6483
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6594..7182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7083..7102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7336..7353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7368..7507
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7433..7452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7593..7615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7609..7800)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8609..9691)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9653..9672
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9692..9769)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
       61 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
      121 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
      181 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
      241 acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta
      301 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
      361 aggagatata catatgtcca aaacagatac agctgccgtg gaggcaaccg aagagaactc
      421 gaacgagacg acttcagatg cggccaccag ttcatccggt gaaaaggagg ctgagttcga
      481 caacaaaatc gaggctgatc gcagtaggcg ctttgatttc ctgctaaagc agacggagat
      541 attcacccac ttcatgacta acagcgctaa gagtcccacg aagcctaagg gtagacccaa
      601 gaagatcaaa gacaaggaca aggaaaagga tgtggccgat catcgtcatc gcaagacaga
      661 gcaggaggag gatgaggagt tgctggcgga agactcggcc accaaggaga tctttcgctt
      721 cgatgcctca cccgcctaca tcaaaagtgg agagatgcgt gactaccaga ttcgcggcct
      781 taactggatg atttcgcttt acgaaaatgg tatcaatgga attctggccg atgaaatggg
      841 tctaggaaag accctgcaga ccatatctct gctgggttac ctcaagcatt tcaaaaatca
      901 agctggacca cacatcgtca tcgtgccaaa gtcaacgctt cagaattggg taaatgagtt
      961 taaaaagtgg tgtccttccc tcagagccgt ctgccttatt ggtgaccagg acacccgtaa
     1021 caccttcatt agagatgtgc tcatgcctgg cgagtgggac gtttgcgtga cctcctatga
     1081 gatgtgtatc cgcgagaagt ctgtattcaa gaagttcaac tggcgctatt tggtcatcga
     1141 cgaggcgcat cgtatcaaga acgagaagtc gaagctgtcg gagattctgc gagagtttaa
     1201 gaccgctaat cgtctactta tcacgggtac tccgctgcag aataacctcc acgagctgtg
     1261 ggccctgctt aatttcctgc tgcccgatgt gtttaattcg tcagaggatt ttgacgaatg
     1321 gttcaacacg aacacctgcc tgggtgacga tgcattgatt acgcgtttgc atgccgtgct
     1381 gaaacctttc ctgctccgtc gtctaaaggc cgaagtggag aagcgtctga agccgaagaa
     1441 ggagatgaaa atatttgtgg gtctatccaa gatgcaacgc gactggtaca ccaaggtgct
     1501 gcttaaggac attgatgtag tgaacggtgc tggcaaagtg gagaagatgc gactgcagaa
     1561 catccttatg cagctccgca agtgcaccaa ccacccatat ttgtttgatg gcgccgagcc
     1621 cggtccgcca tacaccacgg acacgcattt ggtgtataac tccggaaaga tggctattct
     1681 ggacaagctg ctgcccaagc tccaagagca gggatcgcgt gtgttgattt tctcacaaat
     1741 gacgaggatg ttggatatcc tcgaggacta ctgtcactgg cgcaactaca actattgccg
     1801 cctggatggt cagacgccgc acgaagatcg taacaggcag attcaggaat ttaacatgga
     1861 caacagcgcc aagtttctct tcatgttgtc cacccgagcc ggtggtttgg gtatcaattt
     1921 ggctaccgct gatgttgtca tcatttacga ctcggattgg aatcctcaaa tggatttgca
     1981 agctatggat cgtgctcatc gtattggtca aaagaagcaa gtgcgcgttt tccggctgat
     2041 caccgaaagt acagtggagg agaagatcgt ggagagagca gaggtcaagc tccgtctgga
     2101 caagatggtc atccaggggg gcagattggt tgacaaccgc tccaatcagt tgaacaagga
     2161 tgaaatgctt aatataatcc gttttggagc taaccaagtg ttcagctcta aggagacaga
     2221 cattaccgat gaggacatcg atgttatttt ggagcgcggt gaggccaaga cggccgagca
     2281 aaaggcagca ctggacagtc tgggcgagag ttcgctgcgg acgttcacaa tggacacaaa
     2341 cggcgaggca ggaacttctt ccgtatatca attcgagggt gaggattggc gcgagaagca
     2401 aaagctaaat gcgctgggca actggatcga gccaccgaag cgtgaacgca aagccaacta
     2461 tgctgtggat gcctatttcc gcgaggctct ccgtgtctcc gaacccaagg caccgaaggc
     2521 tccccgccca cccaagcagc ctatcgttca ggactttcag ttcttcccac cccgtctgtt
     2581 tgagctgctc gaccaggaaa tctactattt ccgcaagact gttggttaca aggtgcccaa
     2641 gaacacggaa ctaggatcgg atgccaccaa agtgcagcgc gaggagcagc gcaagatcga
     2701 tgaggcagag ccgcttaccg aggaagagat ccaggagaag gagaatctac tctcacaggg
     2761 tttcactgcc tggaccaagc gcgatttcaa ccagttcatc aaggctaacg aaaagtacgg
     2821 tcgggatgat attgacaaca ttgccaagga cgtggagggc aagactccgg aggaggttat
     2881 cgagtacaac gccgtgtttt gggagcgatg cactgagttg caggatattg agcgaataat
     2941 gggacagatt gagcgtggag agggcaagat tcaacggcga ttgtctatta agaaggcttt
     3001 ggatcaaaag atgtcgcggt atcgcgcccc cttccatcag ttgcggctgc aatatggtaa
     3061 taacaagggc aagaattaca ctgaaataga ggatcgcttt ctggtatgca tgcttcacaa
     3121 gttgggcttt gacaaggaga atgtctacga agagctgcga gcggctataa gagcttctcc
     3181 gcaattccgc tttgactggt tcatcaaatc tagaaccgct ttggagctac agcgtcgctg
     3241 caacacgttg attaccctta ttgaacgtga aaacattgag ctggaagaga aagaacgcgc
     3301 cgagaaaaag aaaaaggcac caaagggcag cgtgtccgct ggaagtggaa gtgccagctc
     3361 gaacactcca gcacccgcgc cccaaccgaa ggccagtcaa aagcggaaga gcgaggtggt
     3421 ggcaaccagt tccaactcga aaaagaagaa gaagcatatg gggtgctttg ccaagggtac
     3481 caatgtttta atggcggatg ggtctattga atgtattgaa aacattgagg ttggtaataa
     3541 ggtcatgggt aaagatggca gacctcgtga ggtaattaaa ttgcccagag gaagagaaac
     3601 tatgtacagc gtcgtgcaga aaagtcagca cagagcccac aaaagtgact caagtcgtga
     3661 agtgccagaa ttactcaagt ttacgtgtaa tgcgacccat gagttggttg ttagaacacc
     3721 tcgtagtgtc cgccgtttgt ctcgtaccat taagggtgtc gaatattttg aagttattac
     3781 ttttgagatg ggccaaaaga aagcccccga cggtagaatt gttgagcttg tcaaggaagt
     3841 ttcaaagagc tacccaatat ctgaggggcc tgagagagcc aacgaattag tagaatccta
     3901 tagaaaggct tcaaataaag cttattttga gtggactatt gaggccagag atctttctct
     3961 gttgggttcc catgttcgta aagctaccta ccagacttac gctccaattc tttatgagaa
     4021 tgaccacttt ttcgactaca tgcaaaaaag taagtttcat ctcaccattg aaggtccaaa
     4081 agtacttgct tatttacttg gtttatggat tggtgatgga ttgtctgaca gggcaacttt
     4141 ttcggttgat tccagagata cttctttgat ggaacgtgtt actgaatatg ctgaaaagtt
     4201 gaatttgtgc gccgagtata aggacagaaa agaaccacaa gttgccaaaa ctgttaattt
     4261 gtactctaaa gttgtcagag gtaatggtat tcgcaataat cttaatactg agaatccatt
     4321 atgggacgct attgttggct taggattctt gaaggacggt gtcaaaaata ttccttcttt
     4381 cttgtctacg gacaatatcg gtactcgtga aacatttctt gctggtctaa ttgattctga
     4441 tggctatgtt actgatgagc atggtattaa agcaacaata aagacaattc atacttctgt
     4501 cagagatggt ttggtttccc ttgctcgttc tttaggctta gtagtctcgg ttaacgcaga
     4561 acctgctaag gttgacatga atgtcaccaa acataaaatt agttatgcta tttatatgtc
     4621 tggtggagat gttttgctta acgttctttc gaagtgtgcc ggctctaaaa aattcaggcc
     4681 tgctcccgcc gctgcttttg cacgtgagtg ccgcggattt tatttcgagt tacaagaatt
     4741 gaaggaagac gattattatg ggattacttt atctgatgat tctgatcatc agtttttgct
     4801 tggatcccag gttgtcgtcc atgcatgcgg tggcctgacc ggtctgaact caggcctcac
     4861 gacaaatcct ggtgtatccg cttggcaggt caacacagct tatactgcgg gacaattggt
     4921 cacatataac ggcaagacgt ataaatgttt gcagccccac acctccttgg caggatggga
     4981 accatccaac gttcctgcct tgtggcagct tcaatgacgc aataactagc ataacccctt
     5041 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
     5101 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5161 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5221 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5281 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5341 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5401 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5461 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5521 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     5581 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5641 gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     5701 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     5761 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     5821 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     5881 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     5941 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     6001 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     6061 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     6121 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     6181 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     6241 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     6301 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     6361 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     6421 tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc
     6481 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt
     6541 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6601 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6661 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6721 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6781 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6841 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6901 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6961 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7021 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7081 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7141 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7201 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7261 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7321 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7381 tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct
     7441 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     7501 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7561 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7621 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     7681 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     7741 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     7801 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     7861 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     7921 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     7981 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     8041 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     8101 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     8161 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     8221 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc
     8281 ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg
     8341 agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca
     8401 gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg
     8461 catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa
     8521 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
     8581 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8701 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     8761 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     8821 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     8881 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     8941 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     9001 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     9061 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     9121 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     9181 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     9241 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     9301 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     9361 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     9421 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     9481 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     9541 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     9601 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     9661 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     9721 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     9781 cgctctccct ta
//
