LOCUS       sequence-204625-       14581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr II, 
            corresponding to RDE-1 expressed specifically in the intestine, for 
            tissue-specific RNAi..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14581)
  TITLE     Ewbank lab plasmids
REFERENCE   2  (bases 1 to 14581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pNP160
FEATURES             Location/Qualifiers
     source          1..14581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1456..1489)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1489..1500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2922..3245)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5163..5187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5190..6215
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5190..6215
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6216..6240)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6593..6626)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6995..7012
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6996..7012
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12679..12701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12739..12757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12825..12929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12930..12998,12999..13790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13148..13167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13961..14549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14450..14469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       61 taaccgtatt accgcctttg agtgagctga taccagcgga taacaatttc acagggccct
      121 gtcggattat gggctcttct tttatcctct actttctttc tagaaaatgc aaatgttgag
      181 tcagaaattg ctgaatggag tacaactgca atgggtttag cttatgtttc tggaacaatt
      241 actggtggaa ttgtgattga aaagtaaggt tatatttcat ttcaataaat gttaccacag
      301 tataacaaaa acaagttaca ttgttcagag tatgtctgaa tgtattatta ttattgtatt
      361 tgtgcatcag tactgtagtt gtactgaaga agggtttaaa ataatctcaa cgtttttaag
      421 acgagttagt gagaatacgg tcgcagttag gatgtttgga catagtttta ttagataatc
      481 gtgaactaac tcactataaa aacccgctaa ctcattgaat aagatagttt taaacgttca
      541 aaatttgaag acaaaattgt atgaataagt ttacagagtt ggtcgacgca agcttctcct
      601 tctcttcaca tttctcaata atttagctct aattgcattc gtattcttcg caaaaatacg
      661 tattctgatt gacccgatga aatacggatg cctagttgca ttgatcatct acggttatac
      721 ttatgggtga ggtttttcaa atatattttg atttaatcga ataatgaaat ttcagaactg
      781 gagttggtcc aatttcgtgg tttatatcct ctgaactggt acctcaaaaa catcgtagta
      841 ttgctcaatc tgtggcatat tcaatgaata ctgtaatggt ggttatcagt acatttacag
      901 tattacctct atattccgtt attggtagtt atgctttcct tattctatat tcaattcctt
      961 catttataag tatgcttata ttatttcgct acttaccgga aaccaaagga cgagagattc
     1021 atgatattgt gaatgaactt aaaaaaaaat gaaaaccttt gaaaacgttc atacggccga
     1081 aattttactt gatcgagcca attgaacttt tgttaagact tacagaaaag tgagagattt
     1141 tgttacaaaa aagtgagaaa aaagcgaaaa tgaacaggaa aaagaagaaa aatacacccg
     1201 aagtatcata taaaaattaa aaagattgtt tgacctggcg gaactttttt gcaaaaactc
     1261 tgaatatcca tggcacatgc ttcgtgcaaa acaggatcca aatgaatatc aacaagagat
     1321 tcttgaagac gacgagccac ttgctcagag cattgcttat ctcgaatgag acccttgtta
     1381 aaatcatttt tcaaacatta tatgacattg tcgaaatgtc ctcctgattc catgatggta
     1441 gcaaactcac ttcgtataac ttcgtatagt ataccttata cgaagttatc tgccctcgat
     1501 tgtctatctt ccattctcac caagtttcaa caactttatt cagtcattct tctctggttc
     1561 atttcccaac caatcttccc gaataaagcc catctgtcct ttcggccaca ttcctcacct
     1621 acatccgtac tccttatctc ccgttcttat ccagtctttt atcgtctctg cgtctcgcac
     1681 actgtctcgt cggtaatgta aaaaaaaaac aaaaaaaact tgaaaacttg cggaaagatt
     1741 tcctaataaa aggagacgaa tccggtagga aatcgacaag tctctgcttc tcgaagacta
     1801 ggtgagttac atttctttgg ccgaattatt ttgaaatcct aaatttgata atttcagaga
     1861 tgtctgtaaa acttgcgtgt tatgtgacgg cttcggtcac tgtcgccact cttatggtat
     1921 gcttcatgac catgtcaacc atctactcgg aggttgatgg attcagagag aagcttgata
     1981 ccgagatgaa tgtgttcaga gtgagttaaa ttatattttg aattttaaat aatttttaat
     2041 tttctagcaa tctaccaatg gattgtggaa ggacatagtt gtcatcggaa gatctagcaa
     2101 gcgtgtccgt tgtcaatatg aagagaccaa cgctacccca actccacatg ctgatggatc
     2161 cccatctgct ccaccaggtc aaccaccagc agttccacca gtcttcaacc agccaaagac
     2221 tccaaatgga gccaatggaa atggaccaac ctgcaactgc aatgctgata acaagtgccc
     2281 agctggacca tccggaccaa agggagttcc aggagttcca ggactcgacg gagttccagg
     2341 acttgacggt gttccaggag ttggagctga tgatatcgct ccacaacgcg agtctgtcgg
     2401 atgcttcact tgcccacaag gaccagttgg accaccagga gctcttggaa gaccaggacc
     2461 acgtggactt ccaggaccaa gaggacaaaa tggaaaccca ggaagagatg gacaaccagg
     2521 acatccagga gagcaaggat catccggtca aatcggaaag atcggagagc caggaccacc
     2581 aggagagaag ggacgcgacg ccgagcatcc aatcggaaga ccaggaccaa agggaccaag
     2641 aggagatcaa ggaccaacag gaccagctgg acagaacggt cttcacggac caccaggaga
     2701 gccaggaacc gttggaccag aaggaccatc tggaaagcaa ggacgtcaag gaccagacgg
     2761 aacccaggga gagactggac cagacggaag accaggaaag gatgccgagt actgccagtg
     2821 cccagacaag tctccaccat cagaggctgt caacgccaac cgtggataca gaaatatcta
     2881 aattgttggt gttttctaat aaaatatttg agattcagtg agactttttt cttggcggca
     2941 caataaagtt tcctcttttt gttgggtcac tcaacgaaca caatacaaat atttgagcgc
     3001 tcgcattttt ctcaaccttt cgggtttaaa gcacttcaaa atggaattga agagagattg
     3061 tgaaagtaca tcagacagaa tcacattttg atagcattca cttcactcag atgcaaactt
     3121 ttcagtgact ataaaaagtc ataaaataat aacattaaaa aagcaaataa attagcgaga
     3181 gaattttttg acaaaaagaa agaatagtga tagagaagaa gggaatgctt gaaaggattt
     3241 tgcatttatc ttaatcaccg tcttcgagaa gacgaaccat ggcgccagtt tcagaatcca
     3301 agtttctaat atagttcatc acaatgttga cgttggtcca acctccggct tgcataatct
     3361 ctgggatgga aactccagcg cgagccatgt cacgggcagc tccgacacgg gcggaatgtc
     3421 cagaccaagc caagtaacgt tgtccggaat catccttggc tccgtagatg agacggtggg
     3481 tagcttcgaa gattcctgaa aatttatttt cagcttattc ttagctaata tgttgtttaa
     3541 acttaccttc aagagctcga gtcgagagtt gtgatgttgc actcggggca gcaactccgt
     3601 tcttgcgaac acgacagaac aggtagttgt ttggatcatc ggcgactccg gaaaccgaaa
     3661 tccagcgctc aacgagcttg gtaactccaa gggagagcgc tttttcgact ccggcagtcg
     3721 agaccagcgt ttttgtacgt ccgatgtgga tgagcattct tcctccgtct gtgcgactaa
     3781 tatccttgac gcgaatgcgg gcaatttcgg cgatacggag aagtgtgttg taagcaattc
     3841 cgaggaaggc gaggtttcgg atgtcctggc aacgatcgga attttccata agagaacgga
     3901 cctgatcgaa gtcggttctg aaaatttcgt caagtcttag gattgaatac attaatattt
     3961 aaacttacct ttcgaaggcg agggcttgct tggctctctc tccagcatca acattttcct
     4021 tgcggatacg tctcattacg agagatacgg cattagaatc tgatgggcgt ggaagtccag
     4081 atcggcgatg gagcatattg agctgtccga gatgttgttg gatcgtcttc accgccaatc
     4141 cacgagcttg gaggtagagc aagtagtcac gaacgtcttc tggctcggct gggaaccact
     4201 tgcggttgtt aagcttgcac caggcagccc aagaacggca cactgagaga agcatcttcc
     4261 aggtgtgctc ggagaaggct tgtctgtcac ggaacatatc catgaggttc ttgcgaactt
     4321 catcacttgt cgcgtcaact ggaagtgctg gaagattttg atgaacagtg agaagatttg
     4381 acatattttc gaagtttttt agatgcacta gaacaaagcg tgttggcttc ctctgagccc
     4441 gctttcctta tatacccgca ttctgcagcc ttacagaatg ttctagaagg tcctagatgc
     4501 attcgtttga aaatactccc ggtgggtgca aagagacgca gacggaaaat gtatctgggt
     4561 ctctttattg tgtacactac ttttccatgt accgaatgtg agtcgccctc cttttgcaac
     4621 aagcagctcg aatgttctag aaaaaggtgg aaaatagtat aaataccgtt gaaaataaat
     4681 accgaacaac atttgctcta attgtgaaat tagaaatctt caaactataa tcatgtcact
     4741 ttaccactat ttccgtccag ctcaacgttc cgtttttggt gatcattttt gctttcgtcg
     4801 taaatctaca cacgcgtctc ttccgtgcga gagtccaagc cagcagccaa attcgttgac
     4861 tgagtattca acgtttatac gttgtcggca acgagaaata ggaaaatgca tcgggaaatg
     4921 ttcttttttc gattttttcc aaggttttga caaattttac cacgaatttt gctatgtttt
     4981 caattaaaaa atatgttatt caactgtttc tatgaggaaa ataaggcttt gcatgtaatt
     5041 ttcttattca gcataatttt taattaattt gaattttctg tcctaacgtt tattttgttt
     5101 tcttggttat gactgatctg aaattaattt ttgaatttta aggtaatatg tcaggcggtg
     5161 ccgcaagttt gtacaaaaaa gcaggctcca tgaaaaagcc tgaactcacc gcgacgtctg
     5221 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     5281 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     5341 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     5401 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     5461 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     5521 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     5581 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     5641 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     5701 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     5761 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     5821 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     5881 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     5941 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     6001 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     6061 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     6121 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     6181 gacgccccag cactcgtccg agggcaaagg aatagaccca gctttcttgt acaaagtggg
     6241 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
     6301 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
     6361 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
     6421 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
     6481 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
     6541 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt taataacttc
     6601 gtatagtata ccttatacga agttatacta gtatatattt ttatgttttc ttttaaacct
     6661 tcctccatat ttctgaattg aatgttgttg catattgtgt gtccttattt catttccatc
     6721 atccttttgt gtgtttcccc cttccaccaa ttccccccac gtcctaatga aatgaaagcg
     6781 agaagataaa taaacttgca cattgtgttc agatgagagg agcggaatac gaattgggag
     6841 acggaaagag aaaaaaaaat gtttagattt ggattgaatt aattttttag aaagcaagaa
     6901 aaaaaacaca aagagaataa ataaataata ggaaaaaaaa caaatattca gtatttaaaa
     6961 tttgaaaatt caacgacgtt ggcgtcgatc atcctgtaaa acgacggcca gtgcaacaaa
     7021 cgtgggatgt aaccatggaa gtgagaataa ttgatggaat agctaatgag ctgaagctga
     7081 gtagatcaga gactacttta ttctaaaaag tacagtagtt ggaaaattac atgtttcatt
     7141 tctaattttc aaggaaaagc tagtaattac cgtaatcttg tttgtacctg aaattttatg
     7201 tactgcaggt gaccaagtat gtttgaggca tgacttcaca cacctaactg ataaaggcct
     7261 ctatcacaaa ctagagttgt gacggaaaat tcaacttctc agaatatagc tcaaaatcta
     7321 tcaaatttta ttttcaaaaa tccaaataat tgtgcacgca atgtacttac tgcttcataa
     7381 agttcagaag aattggataa atttgaatga agttttcaaa gcttttatca gtgactgtac
     7441 attgtgatag gcttgtgctg ttatcagctg cctcaaatag gttgtcgctt gaaaatttat
     7501 ataaaaggcc taccagcaga catgagaatc aagcttcaaa ggctctacta gtgatgaaac
     7561 atgtcctcga attttcccga attggaaaaa ggattttatc gtcattctct cgatccggta
     7621 tgatcaatta ttagcagcta taagatatat aagtttgata ttaatattat aggagatgaa
     7681 atggcttgcg aggcccactg gtaaatgcga cggcaaattc tatgagaaga aagtacttct
     7741 tttggtaaat tggttcaagt tctccagcaa aatttacgat cgggaatact acgagtatga
     7801 agtgaaaatg acaaaggaag tattgaatag aaaaccagga aaacctttcc caaaaaagac
     7861 agaaattcca atgtaagtgc ttgtaaatta gtcaaaacta attttatttt tcagtcccga
     7921 tcgtgcaaaa ctcttctggc aacatcttcg gcatgagaag aagcagacag attttattct
     7981 cgaagactat gtttttgatg aaaaggacac tgtttatagt gtttgtcgac tgaacactgt
     8041 cacatcaaaa atgctggttt cggagaaagt agtaaaaaag gattcggaga aaaaagatga
     8101 aaaggatttg gagaaaaaaa tcttatacac aatgatactt acctatcgta aaaaatttca
     8161 cctgaacttt agtcgagaaa atccggaaaa agacgaagaa gcgaatcgga gttacaaatt
     8221 cctgaaggtt tatgaaaaac acgcattata acaaacaaaa ttagctttca gaatgttatg
     8281 acccagaaag ttcgctacgc gccttttgtg aacgaggaga ttaaagtgtg agttgcaata
     8341 ataataataa taatcacctc aactcattta tatattttaa gacaattcgc gaaaaatttt
     8401 gtgtacgata ataattcaat tctgcgagtt cctgaatcgt ttcacgatcc aaacagattc
     8461 gaacaatcat tagaagtagc accaagaatc gaagcatggt ttggaattta cattggaatc
     8521 aaagaattgt tcgatggtga acctgtgctc aattttgcaa gtaagtttga gaaactgcga
     8581 taaaaaatca tgtgattttt gttgaagttg tcgataaact attctacaat gcaccgaaaa
     8641 tgtctcttct ggattatctt ctcctaattg tcgaccccca gtcgtgtaac gatgatgtac
     8701 gaaaagatct taaaacaaaa ctgatggcgg gaaaaatgac aatcagacaa gccgcgcggc
     8761 caagaattcg acaattattg gaaaatttga agctgaaatg cgcagaagtt tgggataacg
     8821 aaatgttagt ttaaattatt caaacaatta atatacaaat tgattttcag gtcgagattg
     8881 acagaacgac atctgacatt tctagatttg tgcgaggaaa actctcttgt ttataaagtc
     8941 actggtaaat cggacagagg aagaaatgca aaaaagtacg atactacatt gttcaaaatc
     9001 tatgaggaaa acaaaaagtt cattgagttt ccccacctac cactagtcaa agttaaaagt
     9061 ggagcaaaag aatacgctgt accaatggaa catcttgaag ttcatgagaa gccacaaaga
     9121 tacaagaatc gaattgatct ggtgatgcaa gacaagtttc taaagcgagc tacacgaaaa
     9181 cctcacgact acaaagaaaa taccctaaaa atgctgaaag aattggattt ctcttctgaa
     9241 gagctaaatt ttgttgaaag atttggatta tgctccaaac ttcagatgat cgaatgtcca
     9301 ggaaaggttt tgaaagagcc aatgcttgtg aatagtgtaa atgaacaaat taaaatgaca
     9361 ccagtgattc gtggatttca agaaaaacaa ttgaatgtgg ttcccgaaaa agaactttgc
     9421 tgtgctgttt ttgtagtcaa cgaaacagcg ggaaatccat gcttagaaga gaacgacgtt
     9481 gtgtaagtgt tttctacgta gattattccg aaatattttc agtaagttct acaccgaact
     9541 aattggtggt tgcaagttcc gtggaatacg aattggtgcc aatgaaaaca gaggagcgca
     9601 atctattatg tacgacgcga cgaaaaatga atatgccgta agtttcagaa aattgaaagt
     9661 ttttaaatat catatttaca gttctacaaa aattgtacac taaataccgg aatcggtaga
     9721 tttgaaatag ccgcaacaga agcgaagaat atgtttgaac gtcttcccga taaagaacaa
     9781 aaagtcttaa tgttcattat catttccaaa cgacaactga atgcttacgg ttttgtgaaa
     9841 cattattgcg atcacaccat cggtgtagct aatcagcata ttacttctga aacagtcaca
     9901 aaagctttgg catcactaag gcacgagaaa ggatcaaaac gaattttcta tcaaattgca
     9961 ttgaaaatca acgcgaaatt aggaggtatt aaccaggagc ttgactggtc agaaattgca
    10021 gaaatatcac cagaagaaaa agaaagacgg aaaacaatgc cattaactat gtatgttgga
    10081 attgatgtaa ctcatccaac ctcctacagt ggaattgatt attctatagc ggctgtagta
    10141 gcgagtatca atccaggtgg aactatctat cgaaatatga ttgtgactca agaagaatgt
    10201 cgtcccggtg agcgtgcagt ggctcatgga cgggaaagaa cagatatttt ggaagcaaag
    10261 ttcgtgaaat tgctcagaga attcgcagaa gtgagttgtc ttgagtattt aaaagatctc
    10321 tgggattttt aatttttttg taaactttca gaacaacgac aatcgagcac cagcgcatat
    10381 tgtagtctat cgagacggag ttagcgattc ggagatgcta cgtgttagtc atgatgagct
    10441 tcgatcttta aaaagcgaag taaaacaatt catgtcggaa cgggatggag aagatccaga
    10501 gccgaagtac acgttcattg tgattcagaa aagacacaat acacgattgc ttcgaagaat
    10561 ggaaaaagat aagccagtgg tcaataaaga tcttactcct gctgaaacag atgtcgctgt
    10621 tgctgctgtt aaacaatggg aggaggatat gaaagaaagc aaagaaactg gaattgtgaa
    10681 cccatcatcc ggaacaactg tggataaact tatcgtttcg aaatacaaat tcgatttttt
    10741 cttggcatct catcatggtg tccttggtac atctcgtcca ggacattaca ctgttatgta
    10801 tgacgataaa ggaatgagcc aagatgaagt ctatgtaagc gttttgaata gcagttagcg
    10861 attttaggat tttgtaatcc gcatatagtt attataaaaa aatgtttcag aaaatgacct
    10921 acggacttgc ttttctctct gctagatgtc gaaaacccat ctcgttgcct gttccggttc
    10981 attatgctca tttatcatgt gaaaaagcga aagagcttta tcgaacttac aaggaacatt
    11041 acatcggtga ctatgcacag ccacggactc gacacgaaat ggaacatttt ctccaaacta
    11101 acgtgaagta ccctggaatg tcgttcgcat aacattttgc aaaagtgtcg cccgtttcaa
    11161 tcaaattttt caattgtaga tattgtactt actttttttt aaagcccggt ttcaaaaatt
    11221 cattccatga ctaacgtttt cataaattac ttgaaattta ttctgtgttt attatttatt
    11281 acctctaaat ttcgttttga acgtgagcat catatcttaa actacttatt gataacggtt
    11341 tcataaagat aggtaatttg atttttaaaa tgttttctct ctgcaggtga tttcacgact
    11401 atgcgcgatg ccgtatcaca actgagaaaa aagacaaaac ttattgaaca gtgttccttt
    11461 caaaaaatta ttttgtaaaa aatcgagttt ttctccaaaa taacgcacgc aatcaaaaac
    11521 aagaaaacta acgtgttctc ctgggcgtgg aactccttat caagattacc attattgtct
    11581 gtcagaattg atatttcaat tatttcatat ctagacaaac ccgggtgtct tgcatttatt
    11641 agtactgtga gtttacatac aaacgaggct tactcagata acatagagtc acctctaatt
    11701 agcaaaaacc catttctaat tagctatttc gaccagttct aaagtcaccg taattagaaa
    11761 tggtttttgt agcttttcta attagaaatg accccgagat aagtgaaata tggcattttc
    11821 tgggttttct agtaagaaat gatcccgcac gtagtgattc cgcgttacaa gccaaattca
    11881 aattaaaaaa gtatattaca caattcaagt tacattattt tcaggaaaca atggcgaaga
    11941 gcaaagcaga gattcaaaag gaggaaagag acaacagata tgaacgaaga cttcttgaaa
    12001 agagaaagag acatgaggtg agtaaccatg ataataaata atcaagtaag ggctatagat
    12061 ttataaattc tggagttaac agtttcgaaa aaaagtagtt ttcatatgtg cgaaggaata
    12121 tcaaaaatag gggaatagcc taaacgagtg agaaaattaa atgtttgtta ttttttcaaa
    12181 aatgtctgca gggccgttat cctttcaaat gcactctatc aaacatagat catatccatc
    12241 atatcttatc cagaatttat atcatatcca agattgagag cgtttcaagg aaaatctcaa
    12301 acaaccatac aattttgtag atcgacaaca aggcctccac ttcttccacg tgacgtcgtg
    12361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    12421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    12481 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
    12541 gcatatggtg cactctcagt gcaatctgct ctgatgccgc atagttaagc cagccccgac
    12601 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    12661 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    12721 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
    12781 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    12841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    12901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    12961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    13021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    13081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    13141 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    13201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    13261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    13321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    13381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    13441 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    13501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    13561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    13621 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggtcagatg
    13681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    13741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    13801 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    13861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    13921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    13981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    14041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    14101 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    14161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    14221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    14281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    14341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    14401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    14461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    14521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    14581 t
//
