LOCUS       sequence-204646-        8757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Scot Wolfe
  TITLE     Inducible Cas9-DNA-binding-domain fusions for precise genome editing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL
FEATURES             Location/Qualifiers
     source          1..8757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..50
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     32..49
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             154..180
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             244..270
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             292..4392
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4405..4425
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4441..4443,4444..4764)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     polyA_signal    4806..4940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4830..4849)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4884..4903
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5053..5073)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5053..5071)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5092..5108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5092..5108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5105..5127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5116..5132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5140..5146,5147..5164,5165..5170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5185..5206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5323..5340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5494..6082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5574..5593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6253..7044,7045..7113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6876..6895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7114..7218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7244..7699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7381..7402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7593..7612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7770..8754
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cattctgcct ggggacgtcg gagcaagctt gatttaggtg acactataga atacaagcta
       61 cttgttcttt ttgcaggatc ccatcgattc gaattcaagg atcaacaagt ttgtacaaaa
      121 aagcaggctg gcgccaccat gggtagagcc gcgccggcag ctaagaaaaa gaaactggat
      181 caattgggcg ctagcggtag tcccgggtct ggaggtagcg gctcaagtgg ccgtacggca
      241 gctcctgcgg caaaaaagaa aaagttggac tctgaattcg gaagcggaag cgacaagaag
      301 tactccattg ggctcgatat cggtaccaac agcgtcggct gggccgtcat tacggacgag
      361 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
      421 aagaacctca ttggagccct cctgttcgac tccggggaga cggccgaagc cacgcggctc
      481 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
      541 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
      601 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
      661 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gctggtagac
      721 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcacat gatcaaattt
      781 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
      841 tttatccaac tggttcagac ttacaatcag cttttcgagg agaacccgat caacgcatcc
      901 ggcgttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac
      961 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
     1021 tcactcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctg
     1081 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac
     1141 cagtacgcag accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat
     1201 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc
     1261 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct
     1321 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac
     1381 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac
     1441 ggcaccgagg agctgctggt aaagctgaac agagaagatc tgttgcgcaa acagcgcact
     1501 ttcgacaatg gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg
     1561 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc
     1621 acatttcgga taccctacta tgtaggcccc ctcgctcggg gaaattccag attcgcgtgg
     1681 atgactcgca aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag
     1741 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac
     1801 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc
     1861 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag
     1921 aagaaagcta tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc
     1981 aaagaagact atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag
     2041 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag
     2101 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg
     2161 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac
     2221 gacaaagtca tgaaacagct caagagacgc cgatatacag gatgggggcg gctgtcaaga
     2281 aaactgatca atggcatccg agacaagcag agtggaaaga caatcctgga ttttcttaag
     2341 tccgatggat ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt
     2401 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc
     2461 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
     2521 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
     2581 cgagagaacc aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
     2641 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
     2701 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
     2761 gatcaggaac tggacatcaa ccggttgtcc gactacgacg tggatcatat cgtgccccaa
     2821 agctttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaataga
     2881 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
     2941 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
     3001 cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca
     3061 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
     3121 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca
     3181 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
     3241 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctg
     3301 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
     3361 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
     3421 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
     3481 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgc
     3541 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc
     3601 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
     3661 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
     3721 gttgtggcca aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
     3781 ggcatcacaa tcatggagcg atccagcttc gagaaaaacc ccatcgactt tctcgaagcg
     3841 aaaggatata aagaggtcaa aaaagacctc atcattaagc tgcccaagta ctctctcttt
     3901 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac
     3961 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
     4021 ctcaaagggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
     4081 taccttgatg agatcatcga gcaaataagc gagttctcca aaagagtgat cctcgccgac
     4141 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
     4201 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
     4261 aagtacttcg acaccaccat agacagaaag aagtacacct ctacaaagga ggtcctggac
     4321 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
     4381 ctcggtggag acggcaccgg cgggcccaag aagaagagga aggtaatcgg atcctccgga
     4441 atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc
     4501 cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctct
     4561 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
     4621 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
     4681 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
     4741 gatgtggagc ttctaaaact ggaactcgag taataatcta gaactatagt gagtcgtatt
     4801 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4861 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4921 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4981 gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc
     5041 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     5101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat
     5161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     7261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     7501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7681 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     7741 tcggtgcggg cctcttcgct attacgccag tcgaccgcca attcaatatg gcgtatatgg
     7801 actcatgcca attcaatatg gtggatctgg acctgtgcca attcaatatg gcgtatatgg
     7861 actcgtgcca attcaatatg gtggatctgg accccagcca attcaatatg gcggacttgg
     7921 caccatgcca attcaatatg gcggacctgg cactgtgcca actggggagg ggtctacttg
     7981 gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc aagtccgcca tattgaattg
     8041 gcatggtgcc aataatggcg gccatattgg ctatatgcca ggatcaatat ataggcaata
     8101 tccaatatgg ccctatgcca atatggctat tggccaggtt caatactatg tattggccct
     8161 atgccatata gtattccata tatgggtttt cctattgacg tagatagccc ctcccaatgg
     8221 gcggtcccat ataccatata tggggcttcc taataccgcc catagccact cccccattga
     8281 cgtcaatggt ctctatatat ggtctttcct attgacgtca tatgggcggt cctattgacg
     8341 tatatggcgc ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc
     8401 attgacgtca ataggaccac ccaccattga cgtcaatggg atggctcatt gcccattcat
     8461 atccgttctc acgcccccta ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat
     8521 caatatctat taatagtaac ttggcaagta cattactatt ggaagtacgc cagggtacat
     8581 tggcagtact cccattgacg tcaatggcgg taaatggccc gcgatggctg ccaagtacat
     8641 ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg
     8701 ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg gaaccgc
//
