LOCUS       sequence-204694-        9268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSIN - EF-1alpha - sFD - P2A - MTS - sFNR, vector encoding for 
            Synechococcus sp. Ferredoxin (Fd) and Ferredoxin-oxireductase (FNR) 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-233
FEATURES             Location/Qualifiers
     source          1..9268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..1180
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          233..1171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1128..1148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1280..1354
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      1376..1385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1385..1426
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1739..1795
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1796..1870
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1901..1918
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3148..3168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3153..3482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3333..3468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3492..4091
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3492..3511)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3988..4008
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4384..4617
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4768..4785)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4774..4998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      5041..5050
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5094..5198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5199..5267,5268..6059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5417..5436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6230..6818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6719..6738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6977..7610
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7657..7782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8275..8508
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8693..8737
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9024..9141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      361 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      421 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      481 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      541 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      601 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      661 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      721 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      781 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      841 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
      901 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
      961 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1021 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1081 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1141 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattgatc cttcgaacta
     1201 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagttcgaag gatccgctag
     1261 cacattcgaa gccgccacca tgtccgtctt gacaccattg ttgttgagag ggttgacagg
     1321 ttcagccagg agattgccag tgcccagagc caaaatacat tcattattcg aagccgccac
     1381 catgggcaag cccatcccca accccctgct gggcctggac tccaccgtta acatggctac
     1441 ttacaaagtg accctgatta cacccgatgg ggaggtgtcc tacgatgccc ccgacgatga
     1501 gtatattctg gacagcgccg gcgatgcagg gtacgatctg ccagcttctt gcagagctgg
     1561 cgcctgcagt acctgcgcag ggaaaatcgt gagcggaaca gtggatcaga gtgaacagtc
     1621 cttcctggac gatgaccaga tcgaggccgg ctatgtgctg acatgtattg cctacccaca
     1681 gtccgatgtg accattgaga ccaacaagga ggaagagctg tacgtgtacg gaagcggagc
     1741 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgtc
     1801 cgtcttgaca ccattgttgt tgagagggtt gacaggttca gccaggagat tgccagtgcc
     1861 cagagccaaa atacattcat tagaattcgc cgccaccatg catcatcacc accaccatgt
     1921 taacatgtat ggaatcacct caaccgccaa ctcaaccgga aatcagtcct acgctaatcg
     1981 gctgttcatc tacgaagtgg tcgggctggg aggcgacgga cggaacgaga atagcctggt
     2041 gcgcaagtcc gggaccacat tcatcactgt cccttacgca cgaatgaacc aggaaatgca
     2101 gaggattacc aagctgggcg gaaaaatcgt gtctattcga ccagccgagg atgccgctca
     2161 gatcgtcagt gaaggacaga gctccgctca ggcatcagcc cagagcccta tggccagcag
     2221 cacaaagatt gtgcacccca aaactaccga cactagcgtg cctgtcaaca tctaccgccc
     2281 taagacccca ttcctgggca aatgcattga gaattatgaa ctggtggatg aggggggctc
     2341 aggaactgtg aggcatgtca cctttgacat cagcgagggg gatctgagat acctggaagg
     2401 gcagtccatt ggcatcattc cccctggaga ggacaaaaac gggaagcctc acaaactgcg
     2461 cctgtattcc atcgcctcta cccgacatgg cgacatggag gataataaga ccgtgagcct
     2521 gtgcgtgagg cagctggaat accaggaccc cgagtctggc gaaaccgtgt acggcgtgtg
     2581 cagcacatac ctgtgcaacc tgccagtggg aacagacgat gtcaagatca ctggaccagt
     2641 ggggaaagag atgctgctgc ccgacgatga agacgcaaca gtggtcatgc tggccacagg
     2701 gactggcatt gccccattca gggcttttct gtggagaatg tttaaggagc agcacgaaga
     2761 ttataagttc aaagggaagg cctggctgat ctttggcgtg ccctacactg ctaatattct
     2821 gtataaggac gatttcgaga aaatggcagc cgaaaaccct gacaacttca ggctgactta
     2881 cgctatctcc cgggagcaga agaccgcaga cggagggaaa gtgtacgtcc agagtcgggt
     2941 gtcagaatat gccgatgagc tgtttgaaat gatccagaag cccaacacac acgtgtacat
     3001 gtgcggcctg aaaggaatgc agccacccat tgacgagacc tttacagctg aggcagaaaa
     3061 gcgcggcctg aattgggagg aaatgaggag gtctatgaaa aaagagcatc ggtggcatgt
     3121 ggaggtgtat tgataagtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3241 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3301 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3361 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3421 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3481 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     3541 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     3601 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     3661 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     3721 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     3781 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     3841 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     3901 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     3961 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4021 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     4081 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     4141 atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggcg gccgctcgag
     4201 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4261 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     4321 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4381 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4441 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4501 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4561 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4621 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4681 gtgagaggcc ttgacattat aatagattta gcaggaattg aactaggagt ggagcacaca
     4741 ggcaaagttc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4801 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4861 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4921 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4981 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcgc gcccctcgag
     5041 gccgccatgg tcatagctgt ttgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcacg agacaataac
     5161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6841 tacggttctg gccttttgca cggaccgcca ctgccaatta cctgtggttt catttactct
     6901 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     6961 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     7021 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7081 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7141 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7201 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     7261 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     7321 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     7381 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     7441 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7501 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7561 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7621 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7681 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7741 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7801 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7861 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7921 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7981 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8041 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8101 aagaccaccg cacagcaacc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8161 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8221 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8281 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8341 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8401 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8461 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8521 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8581 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8641 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8701 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8761 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8821 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8881 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8941 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca
     9001 caaatggcag tattcatcca caattttaaa agaaaggggg ggattggggg gtacagtgca
     9061 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     9121 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggatc
     9181 gataagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     9241 ccttctaggt cttgaaagga gtgggaat
//
